AFM Multimode Imaging and Nanoindetation Method for Assessing Collagen Nanoscale Thin Films Heterogeneity

Stylianou, Andreas; Kontomaris, Stylianos Vasileios; Yova, Dido; Balogiannis, Georgios

doi:10.1007/978-3-319-00846-2_101

Cited by 8 publications

(7 citation statements)

References 21 publications

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“…The stiffness value leads to the estimation of the Young's modulus, under the condition that the shape of the tip and the Poisson's ratio of the sample are known. The formula that contains both the stiffness and the Young's modulus is:

E = \frac{\sqrt{π}}{2} (1 - v^{2}) \frac{S}{\sqrt{A}}

where, E is the Young's modulus, ν the Poisson's ratio, S the contact stiffness, and A an area function related to the effective cross‐sectional or projecting area of the indenter (Oliver and Pharr, ; Fischer‐Cripps, ; Sirghi, ; Stylianou et al, ). Regarding small indentation depths, pyramidal indenters can be analyzed in terms of a paraboloid of revolution which can be approximated by a sphere (Oliver and Pharr, ).…”

Section: Methodsmentioning

confidence: 99%

The effects of UV irradiation on collagen D-band revealed by atomic force microscopy

et al. 2014

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The objective of this paper was to investigate the influence of UV irradiation on collagen D-band periodicity by using the AFM imaging and nanoindentation methods. It is well known than UV irradiation is one of the main factors inducing destabilization of collagen molecules. Due to the human's skin chronic exposure to sun light, the research concerning the influence of UV radiation on collagen is of great interest. The impact of UV irradiation on collagen can be studied in nanoscale using Atomic Force Microscopy (AFM). AFM is a powerful tool as far as surface characterization is concerned, due to its ability to relate high resolution imaging with mechanical properties. Hence, high resolution images of individual collagen fibrils and load-displacement curves on the overlapping and gap regions, under various time intervals of UV exposure, were obtained. The results demonstrated that the UV rays affect the height level differences between the overlapping and gap regions. Under various time intervals of UV exposure, the height difference between overlaps and gaps reduced from ~3.7 nm to ~0.8 nm and the fibril diameters showed an average of 8-10% reduction. In addition, the irradiation influenced the mechanical properties of collagen fibrils. The Young's modulus values were reduced per 66% (overlaps) and 61% (gaps) compared to their initial values. The observed alterations on the structural and the mechanical properties of collagen fibrils are probably a consequence of the polypeptide chain scission due to the impact of the UV irradiation.

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E = \frac{\sqrt{π}}{2} (1 - v^{2}) \frac{S}{\sqrt{A}}

Section: Methodsmentioning

confidence: 99%

The effects of UV irradiation on collagen D-band revealed by atomic force microscopy

et al. 2014

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“…AFM permits quantitative, high-resolution, non-destructive imaging of surfaces, including biological ones [25,26]. In addition, it operates in many different modes, offering a vast amount of information about bio-samples [27][28][29] ranging from topography to mechanical properties characterization [30][31][32][33]. AFM has been used in the field of cancer research, mainly due to its ability to work on live cells with high resolution, and its potential to provide quantitative information (e.g.…”

Section: Introductionmentioning

confidence: 99%

Atomic Force Microscopy Probing of Cancer Cells and Tumor Microenvironment Components

Stylianou

Stylianopoulos

2015

BioNanoSci.

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Cancer cells have different characteristics from normal cells in terms of morphology, cell-cell interactions, cytoskeleton organization, cell growth rates, and cell-extracellular matrix interactions. Although the clarification of these characteristics is crucial for the effective development of new therapeutic strategies, they remain poorly identified. Furthermore, tumor microenvironment plays a crucial role in cancer progression and metastasis, and thus, it is of utmost importance to decode and fully understand the crosstalk and the interactions between its different components. Atomic Force Microscopy (AFM) is an established and multifunctional tool in biomedical sciences with many applications in cancer research. In this review, we discuss the use of AFM in the study of cancer cells and the tumor microenvironment. We first provide a brief overview of AFM operating principles highlighting its contribution to the study of cancer and stromal cells. Next, we present research on the use of AFM in the study of celltumor microenvironment interactions during cancer cell migration and invasion. Finally, we discuss the development of coupled AFM modalities and the combination of different kinds of imaging with AFM that have been applied to cancer research.

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“…They are well-aligned on the samples cultured in BM and randomly distributed on the samples cultured in OM, which suggests that the samples from BM will show anisotropic mechanical properties and the samples from OM will show relatively isotropic mechanical properties [44].…”

Section: Surface Analysismentioning

confidence: 88%

How cell culture conditions affect the microstructure and nanomechanical properties of extracellular matrix formed by immortalized human mesenchymal stem cells: An experimental and modelling study

Duan

Toumpaniari

Partridge

et al. 2018

Materials Science and Engineering: C

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This paper presents an investigation of how different culture media (i.e. basal and osteogenic media) affect the nanomechanical properties and microstructure of the mineralized matrix produced by the human mesenchymal stem cell line Y201, from both an experimental and theoretical approach. A bone nodule (i.e. mineralized matrix) cultured from basal medium shows a more anisotropic microstructure compared to its counterpart cultured from an osteogenic medium. As confirmed by finite element simulations, this anisotropic microstructure explains the bimodal distribution of the corresponding mechanical properties very well. The overall nanomechanical response of the bone nodule from the osteogenic medium is poorer compared to its counterpart from the basal medium. The bone nodules, from both basal and osteogenic media, have shown reverse aging effects in terms of mechanical properties. These are possibly due to the fact that cell proliferation outcompetes the mineralization process.

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AFM Multimode Imaging and Nanoindetation Method for Assessing Collagen Nanoscale Thin Films Heterogeneity

Cited by 8 publications

References 21 publications

The effects of UV irradiation on collagen D-band revealed by atomic force microscopy

The effects of UV irradiation on collagen D-band revealed by atomic force microscopy

Atomic Force Microscopy Probing of Cancer Cells and Tumor Microenvironment Components

How cell culture conditions affect the microstructure and nanomechanical properties of extracellular matrix formed by immortalized human mesenchymal stem cells: An experimental and modelling study

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