AFM Force Spectroscopy Reveals the Role of Integrins and Their Activation in Cell–Biomaterial Interactions

Harjumäki, Riina; Zhang, Xue; Nugroho, Robertus Wahyu N.; Farooq, Muhammad; Lou, Yan-Ru; Valle‐Delgado, Juan José; Österberg, Monika

doi:10.1021/acsabm.9b01073

Cited by 20 publications

(20 citation statements)

References 55 publications

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“…To this end, we analyzed the maximum adhesion force of single cells and measured the total work required to completely detach the cell from the substrate to quantify the bioactivity of the different surfaces. Adhesion on longer time scales goes in line with maturation of specific cell-surface binding sites, e.g., via integrin receptors [37] which correlates with cell spreading and the formation of neurites. Therefore, we further studied the dynamics of spreading and changes in cell growth areas over several days of cultured SH-SY5Y and U-87 MG cells.…”

Section: Introductionmentioning

confidence: 96%

Adhesion of Neurons and Glial Cells with Nanocolumnar TiN Films for Brain-Machine Interfaces

Abend

Steele

Jahnke

et al. 2021

IJMS

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Coupling of cells to biomaterials is a prerequisite for most biomedical applications; e.g., neuroelectrodes can only stimulate brain tissue in vivo if the electric signal is transferred to neurons attached to the electrodes’ surface. Besides, cell survival in vitro also depends on the interaction of cells with the underlying substrate materials; in vitro assays such as multielectrode arrays determine cellular behavior by electrical coupling to the adherent cells. In our study, we investigated the interaction of neurons and glial cells with different electrode materials such as TiN and nanocolumnar TiN surfaces in contrast to gold and ITO substrates. Employing single-cell force spectroscopy, we quantified short-term interaction forces between neuron-like cells (SH-SY5Y cells) and glial cells (U-87 MG cells) for the different materials and contact times. Additionally, results were compared to the spreading dynamics of cells for different culture times as a function of the underlying substrate. The adhesion behavior of glial cells was almost independent of the biomaterial and the maximum growth areas were already seen after one day; however, adhesion dynamics of neurons relied on culture material and time. Neurons spread much better on TiN and nanocolumnar TiN and also formed more neurites after three days in culture. Our designed nanocolumnar TiN offers the possibility for building miniaturized microelectrode arrays for impedance spectroscopy without losing detection sensitivity due to a lowered self-impedance of the electrode. Hence, our results show that this biomaterial promotes adhesion and spreading of neurons and glial cells, which are important for many biomedical applications in vitro and in vivo.

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Section: Introductionmentioning

confidence: 96%

Adhesion of Neurons and Glial Cells with Nanocolumnar TiN Films for Brain-Machine Interfaces

Abend

Steele

Jahnke

et al. 2021

IJMS

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“…This technique is known as the force mapping method and has become a relatively common technique. [37][38][39] However, special care must be taken for the present study as the size of the abrasive nanoparticles is less than 50 nm and the PVA brush is not a flat sample.…”

Section: Introductionmentioning

confidence: 99%

“…By modifying the tip apex to mimic the structure and/or surface properties of a target nanoparticle and taking force curves at xy -arrayed positions of the surface, the nanoscale distribution of the adhesion force acting between the nanoparticle and the surface can be measured with pico-Newton force sensitivity. This technique is known as the force mapping method and has become a relatively common technique. − However, special care must be taken for the present study as the size of the abrasive nanoparticles is less than 50 nm and the PVA brush is not a flat sample.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Silica Nanoparticle Adhesion to Poly(vinyl alcohol) Surfaces by Ammonia-Mediated Hydration: Implications for Effective Post-Chemical–Mechanical Planarization Cleaning

Ikarashi

Yoshino

Nakajima

et al. 2020

ACS Appl. Nano Mater.

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Adhesion of silica abrasive nanoparticles to a poly(vinyl alcohol) (PVA) brush surface in a post-CMP (chemical mechanical planarization) cleaning leads to serious 1 problems in yield enhancement of semiconductor fabrication. However, the nanoscale adhesion mechanism and its correlation with process conditions have hardly been understood. In this study, we investigated the influence of ammonia in the cleaning solution on silica nanoparticle adhesion to a PVA surface. By atomic force microscopy (AFM), we directly measured adhesion forces between a nanoscale silica probe and a PVA brush surface in various solutions and found that ammonia has a significant inhibitory effect against silica nanoparticle adhesion to a PVA surface. Importantly, we found that this effect cannot be explained by the electrostatic interactions alone, but also involves steric repulsion between silica and hydrated PVA. We also performed molecular-scale three-dimensional scanning force microscopy (3D-SFM) imaging and contact angle measurements, and found that ammonia promotes hydration and swelling of PVA. Furthermore, we performed molecular dynamics simulations and found that ammonia promotes dynamic rearrangements of hydrogen bonding networks (HBNs) at a PVA-water interface, giving extra flexibility to the PVA chains. Such flexibility promotes local swelling of PVA and inhibits silica nanoparticle adhesion to a PVA surface. This provides important guidelines for optimizing nanoscale structures and interactions of brush surfaces and abrasive nanoparticles in post-CMP cleaning.

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“…Obviously, the boronized Ti6Al4V/HA composite could facilitate osteoblast differentiation. It could be ascribed to its special Ca-containing surface composition, porous structure, and relatively rough micro-morphology, as well as the wnt/β-catenin and BMP/SMAD signal transduction system activated by integrin and matrix protein in the cells [ 19 , 20 ].…”

Section: Resultsmentioning

confidence: 99%

Osteogenesis Performance of Boronized Ti6Al4V/HA Composites Prepared by Microwave Sintering: In Vitro and In Vivo Studies

et al. 2022

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The boronized Ti6Al4V/HA composite is deemed to be an important biomaterial because of its potential remarkable mechanical and biological properties. This paper reports the osteogenesis performance of the boronized Ti6Al4V/HA composite, which was prepared by microwave sintering of powders of Ti6Al4V, hydroxyapatite (HA), and TiB2 in high-purity Ar gas at 1050 °C for 30 min, as dental implant based on both cell experiments in vitro and animal experiments in vivo. The comparison between the boronized Ti6Al4V/HA composite and Ti, Ti6Al4V, and boronized Ti6Al4V in the terms of adhesion, proliferation, alkaline phosphate (ALP) activity, and mineralization of MG-63 cells on their surfaces confirmed that the composite exhibited the best inductive osteogenesis potential. It exerted a more significant effect on promoting the early osteogenic differentiation of osteoblasts and exhibited the maximum optical density (OD) value in the MTT assay and the highest levels of ALP activity and mineralization ability, primarily ascribed to its bioactive HA component, porous structure, and relatively rough micro-morphology. The in vivo study in rabbits based on the micro-computed tomography (micro-CT) analysis, histological and histomorphometric evaluation, and biomechanical testing further confirmed that the boronized Ti6Al4V/HA composite had the highest new bone formation potential and the best osseointegration property after implantation for up to 12 weeks, mainly revealed by the measured values of bone volume fraction, bone implant contact, and maximum push-out force which, for example, reached 48.64%, 61%, and 150.3 ± 6.07 N at the 12th week. Owing to these inspiring features, it can serve as a highly promising dental implant.

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AFM Force Spectroscopy Reveals the Role of Integrins and Their Activation in Cell–Biomaterial Interactions

Cited by 20 publications

References 55 publications

Adhesion of Neurons and Glial Cells with Nanocolumnar TiN Films for Brain-Machine Interfaces

Adhesion of Neurons and Glial Cells with Nanocolumnar TiN Films for Brain-Machine Interfaces

Inhibition of Silica Nanoparticle Adhesion to Poly(vinyl alcohol) Surfaces by Ammonia-Mediated Hydration: Implications for Effective Post-Chemical–Mechanical Planarization Cleaning

Osteogenesis Performance of Boronized Ti6Al4V/HA Composites Prepared by Microwave Sintering: In Vitro and In Vivo Studies

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