AFM-based indentation method for measuring the relaxation property of living cells

Sheng, Jun-Yuan; Mo, Chi; Li, Guo-Yang; Zhao, Hang; Cao, Yanping; Feng, Xi‐Qiao

doi:10.1016/j.jbiomech.2021.110444

Cited by 6 publications

(4 citation statements)

References 37 publications

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“…The length and width of FluidFM cantilevers (Cytosurge AG, Switzerland) were 200 and 36 μm, respectively, with a spring constant of 2 N/m. In general, the choice of a cantilever spring constant may affect the determination of the relaxation modulus based on finite element modeling and dimensional analysis . A relatively large stiffness constant was chosen to accommodate the large adhesive force between cells and the material surface and to enable us to obtain the true equilibrium modulus .…”

Section: Methodsmentioning

confidence: 99%

“…In general, the choice of a cantilever spring constant may affect the determination of the relaxation modulus based on finite element modeling and dimensional analysis. 47 A relatively large stiffness constant was chosen to accommodate the large adhesive force between cells and the material surface and to enable us to obtain the true equilibrium modulus. 47 The cantilever aperture was 8 μm (as shown in the scanning electron microscope image of the cantilever in Figure S1 in the Supporting Information).…”

Section: ■ Introductionmentioning

confidence: 99%

“…47 A relatively large stiffness constant was chosen to accommodate the large adhesive force between cells and the material surface and to enable us to obtain the true equilibrium modulus. 47 The cantilever aperture was 8 μm (as shown in the scanning electron microscope image of the cantilever in Figure S1 in the Supporting Information). With use of the Cell Adhesion 100 μm workflow in Cytosurge software, approach-pause-grab operations were carried out for each cell.…”

Section: ■ Introductionmentioning

confidence: 99%

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Simultaneous Measurement of Single-Cell Mechanics and Cell-to-Materials Adhesion Using Fluidic Force Microscopy

et al. 2022

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The connection between cells and their substrate is essential for biological processes such as cell migration. Atomic force microscopy nanoindentation has often been adopted to measure single-cell mechanics. Very recently, fluidic force microscopy has been developed to enable rapid measurements of cell adhesion. However, simultaneous characterization of the cell-to-material adhesion and viscoelastic properties of the same cell is challenging. In this study, we present a new approach to simultaneously determine these properties for single cells, using fluidic force microscopy. For MCF-7 cells grown on tissue-culture-treated polystyrene surfaces, we found that the adhesive force and adhesion energy were correlated for each cell. Well-spread cells tended to have stronger adhesion, which may be due to the greater area of the contact between cellular adhesion receptors and the surface. By contrast, the viscoelastic properties of MCF-7 cells cultured on the same surface appeared to have little dependence on cell shape. This methodology provides an integrated approach to better understand the biophysics of multiple cell types.

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Section: Methodsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Simultaneous Measurement of Single-Cell Mechanics and Cell-to-Materials Adhesion Using Fluidic Force Microscopy

et al. 2022

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“…The mechanical properties of cells were detected by AFM to understand cell deformation behavior and biological work. Jun-yuan proposed a protocol that relies on the selection of suitable cantilever bending rigidity to conduct an AFM-based test on cells, so as to measure the decreased relaxation modulus with high accuracy [9]. Sawinee used AFM imaging to examine the cell membrane morphology of E. coli cells treated with the parent KYR and C14-KYR peptides.…”

Section: Introductionmentioning

confidence: 99%

The applications of AFM in the study of cell, molecule, tissue level to understand the structure and function of organisms

Liu

2022

International Conference on Biomedical and Intelligent Systems (IC-BIS 2022)

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To have a more detailed understanding of the structural characteristics, information transmission and other aspects of an organism, it can be started with the three most basic levels of cells, molecules and tissues. Cells are the most basic unit that constitutes an organism. Their structure determines its function. Observing the structure of cells is of great help to understand the function of an organism. Every strange biological phenomenon is the result of intermolecular interaction. Studying the existence significance of each molecule in a biological process can more thoroughly understand the details of the biological process. Tissue is a structure composed of a series of cells and biological macromolecules. The study of tissues can directly reflect the principle of the operation of birth objects. With its high resolution, AFM is widely used in these three levels of research. This article will introduce the screening of cancer cells by AFM at the cell level, the determination of protein-DNA/RNA interaction properties in molecular interactions and the characterization of ECM in lung tissue at the tissue level.

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An updated model including the deflection history of microcantilever for characterizing cellular viscoelastic properties by AFM indentation-relaxation test

Ling,

Zhang,

Shang

et al. 2024

Acta Mech

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AFM-based indentation method for measuring the relaxation property of living cells

Cited by 6 publications

References 37 publications

Simultaneous Measurement of Single-Cell Mechanics and Cell-to-Materials Adhesion Using Fluidic Force Microscopy

Simultaneous Measurement of Single-Cell Mechanics and Cell-to-Materials Adhesion Using Fluidic Force Microscopy

The applications of AFM in the study of cell, molecule, tissue level to understand the structure and function of organisms

An updated model including the deflection history of microcantilever for characterizing cellular viscoelastic properties by AFM indentation-relaxation test

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