AFLP mapping of quantitative trait loci influencing seven head-related traits in broccoli (<i>Brassica oleracea</i>var.<i>italica</i>)

Lin, Kuan‐Hung; Chang, Lien-chung; Lai, C. D.; Lo, Hsiao-Feng

doi:10.1080/14620316.2013.11512964

Cited by 10 publications

(6 citation statements)

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“…The four primer combinations resulted in 409 amplified bands, all of which are polymorphic. These results are superior to the ones observed by Lin et al (2013), studying the genetic diversity among broccoli accessions based on nine primer combinations AFLP; these authors observed a total of 334 amplified bands, being 208 (62.3%) polymorphic. AFLP methodology associated with automated capillary electrophoresis system has been considered an important tool for exploring the genetic divergence in several vegetable species (Cardoso et al, 2018;Constantino et al, 2020;Massucato et al, 2020).…”

Section: Resultscontrasting

confidence: 72%

Genetic variability among broccoli genotypes based on biochemical and molecular traits

et al. 2021

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Broccoli (Brassica oleracea var. italica) is one of the most important vegetables in the world and also shows important functional properties. The present study aimed to characterize 19 broccoli genotypes using biochemical traits (content of total phenolic compounds, total flavonoids and antioxidant activity) and AFLP (Amplified Fragment Length Polymorphism) molecular markers. The experiment was carried out in a greenhouse using a complete randomized block design with three replicates. The phenotypic data were subjected to analysis of variance (p≤0.05), Scott-Knott average clustering method (p≤0.05) and principal component analysis (PCA). Using molecular data, Ward’s hierarchical clustering, Bayesian clustering and principal coordinate analysis (PCoA) were performed. Molecular data showed genetic diversity among the genotypes (three groups), also a wide variability in the total phenolic compounds, total flavonoids content and antioxidant activity using FRAP method. HT3010, Hanabi and Bonanza genotypes showed desirable biochemical traits for the demanding functional food consumers, in addition to being promising genotypes to be exploited in plant breeding programs.

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Section: Resultscontrasting

confidence: 72%

Genetic variability among broccoli genotypes based on biochemical and molecular traits

et al. 2021

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Section: Discussionmentioning

confidence: 99%

“…In broccoli, higher yielding genotypes are preferred for commercial production and vegetative biomass is positively correlated with head biomass (Lin et al, 2013). In a F 2 broccoli × broccoli population, Lin et al (2013) identified biomass QTL on linkage groups C1, C5, C8, and C9, although the authors did not identify candidates associated with these loci. In a GWA study of seedling vigor in B. napus , the vigor candidates Bna.SCO1 , Bna.ARR4 , and Bna.ATE1 were identified by Hatzig et al (2015), although no homologous candidates were located within the BolTBDH VG BCIs.…”

Section: Discussionmentioning

confidence: 99%

Complex Horticultural Quality Traits in Broccoli Are Illuminated by Evaluation of the Immortal BolTBDH Mapping Population

2019

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Improving horticultural quality in regionally adapted broccoli (Brassica oleracea var. italica) and other B. oleracea crops is challenging due to complex genetic control of traits affecting morphology, development, and yield. Mapping horticultural quality traits to genomic loci is an essential step in these improvement efforts. Understanding the mechanisms underlying horticultural quality enables multi-trait marker-assisted selection for improved, resilient, and regionally adapted B. oleracea germplasm. The publicly-available biparental double-haploid BolTBDH mapping population (Chinese kale × broccoli; N = 175) was evaluated for 25 horticultural traits in six trait classes (architecture, biomass, phenology, leaf morphology, floral morphology, and head quality) by multiple quantitative trait loci mapping using 1,881 genotype-by-sequencing derived single nucleotide polymorphisms. The physical locations of 56 single and 41 epistatic quantitative trait locus (QTL) were identified. Four head quality QTL (OQ_C03@57.0, OQ_C04@33.3, OQ_CC08@25.5, and OQ_C09@49.7) explain a cumulative 81.9% of phenotypic variance in the broccoli heading phenotype, contain the FLOWERING LOCUS C (FLC) homologs Bo9g173400 and Bo9g173370, and exhibit epistatic effects. Three key genomic hotspots associated with pleiotropic control of the broccoli heading phenotype were identified. One phenology hotspot reduces days to flowering by 7.0 days and includes an additional FLC homolog Bo3g024250 that does not exhibit epistatic effects with the three horticultural quality hotspots. Strong candidates for other horticultural traits were identified: BoLMI1 (Bo3g002560) associated with serrated leaf margins and leaf apex shape, BoCCD4 (Bo3g158650) implicated in flower color, and BoAP2 (Bo1g004960) implicated in the hooked sepal horticultural trait. The BolTBDH population provides a framework for B. oleracea improvement by targeting key genomic loci contributing to high horticultural quality broccoli and enabling de novo mapping of currently unexplored traits.

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“…From the relevant research effort [37,38,85,86], no markers closely related to the heat stress tolerance have emerged, nor have possible candidate genes been proposed, based on publicly available broccoli genome annotation [87]. Although researchers started from the late 1980s to construct genetic maps, to detect the loci of important traits, such as the disease resistance, head morphology, nutritional components, time of the head's formation and maturation, etc.…”

Section: Impact Groups Specific Impactsmentioning

confidence: 99%

The Impacts of the Emerging Climate Change on Broccoli (Brassica oleracea L. var. italica Plenck.) Crop

2022

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Broccoli (Brassica oleracea L. var. italica Plenck.) is nowadays one of the most important vegetable crops worldwide, with an increasing demand by the market, due to its high nutritional value. Based on the optimal temperature range, its cultivation in the temperate regions takes place from late summer to late spring. Broccoli cultivation nowadays faces many challenges, such as the profitable production throughout the year, as well as during conditions of an increased temperature, induced by the emerging climate change, as well as the possibility of introducing the cultivation to subtropical and tropical areas. The modern genotypes (F1 hybrids) differ significantly among themselves in terms of the requirements for the head’s formation, which, however, has not been fully elucidated. In this direction, breeders have been developing programs internationally since the early 1990s, in order to create genotypes that will be adapted to temperatures higher than the optimal range, having, however, a relatively limited initial germplasm pool. The purpose of this review is to present extensively the temperature requirements in the broccoli crop production and to highlight the impacts of the emerging climate change.

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AFLP mapping of quantitative trait loci influencing seven head-related traits in broccoli (Brassica oleraceavar.italica)

Cited by 10 publications

References 0 publications

Genetic variability among broccoli genotypes based on biochemical and molecular traits

Genetic variability among broccoli genotypes based on biochemical and molecular traits

Complex Horticultural Quality Traits in Broccoli Are Illuminated by Evaluation of the Immortal BolTBDH Mapping Population

The Impacts of the Emerging Climate Change on Broccoli (Brassica oleracea L. var. italica Plenck.) Crop

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