AFLP Fingerprinting in Pigeonpea (Cajanus cajan (L.) Millsp.) and its Wild Relatives

Abstract

Detection of DNA polymorphism in cultivated pigeonpea (Cajanus cajan) and two of its wild relatives Cajanus volubilis and Rhynchosia bracteata is reported here for the first time using amplified fragment length polymorphism (AFLP) fingerprinting. For this purpose, two EcoRI (three selective nucleotides) and 14 MseI (three selective nucleotides) primers were used. The two wild species shared only 7.15% bands with the pigeonpea cultivars, whereas 86.71% common bands were seen among cultivars. Similarly, 62.08% bands were polymorphic between C. volubilis and pigeonpea cultivars in comparison to 63.33% polymorphic bands between R. bracteata and pigeonpea cultivars, and 13.28% polymorphic bands among pigeonpea cultivars. The cluster analysis revealed low polymorphism among pigeonpea cultivars and very high polymorphism between cultivated pigeonpea and its wild relatives. The AFLP analysis also indicated that only one primer combination (EcoRI + ACT and MseI + CTG), at the most any four primer pair combinations, are sufficient for obtaining reliable estimation of genetic diversity in closely related cultivars like pigeonpea material analyzed herein. AFLP analysis may prove to be a useful tool for molecular characterization of pigeonpea cultivars and its wild relatives and for possible use in genome mapping.

Cross-genera amplification of informative microsatellite markers from common bean and lentil for the assessment of genetic diversity in pigeonpea

“…However, these values still present a wide range of similarity index values among the pigeonpea cultivars as compared to previous reports of narrow range of 0.70 to 0.90 with RAPD and 0.82-1.00 with AFLP markers (Ratnaparkhe et al, 1995;Panguluri et al, 2006). The mean estimate of genetic diversity was higher with SSR primers (49 %) than the diversity reported with RAPD (<30 %) and AFLP (<14 %) in previous reports in pigeonpea.…”

“…Studies on molecular diversity in pigeonpea have been limited by the paucity of polymorphic PCR based markers. Though the molecular marker technologies such as random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) and amplified fragment length polymorphism (AFLP) were occasionally employed in pigeonpea but they tend to present reproducibility problems and were inadequate in assessing within species diversity (Nadimpalli et al, 1992;Ratnaparkhe et al, 1995;Panguluri et al, 2006). RAPD markers have also been used for characterization of cytoplasmic male sterility lines in pigeonpea for subsequent use in hybrid development (Souframanien et al, 2003).…”

Genetic Diversity among Pigeonpea (Cajanus cajan L. Millsp.) Genotypes Using Genic SSRs with Putative Function for Drought Tolerance

“…The PIC of SSRs obtained in our study was relatively higher than those reported by Khalekar et al, (2014) and Datta et al, (2013). Several other workers also reported different level of genetic diversity in pigeonpea (Panguluri et al, 2006;Yang et al, 2006;Singh et al, 2013). This variation in genetic diversity is probably attributed to diversification in morphology, use of common ancestors for the development of new cultivars (Panguluri et al, 2006;Yang et al, 2006).…”

“…Several other workers also reported different level of genetic diversity in pigeonpea (Panguluri et al, 2006;Yang et al, 2006;Singh et al, 2013). This variation in genetic diversity is probably attributed to diversification in morphology, use of common ancestors for the development of new cultivars (Panguluri et al, 2006;Yang et al, 2006). The present study also revealed significant positive correlation between PIC values with number of alleles amplified per primer (r = 0.58*, P < 0.05) and gene diversity (r = 0.99**, P < 0.01), and between allele number and gene diversity (r = 0.57*, P<0.05).…”

“…Discovery of molecular markers led to genetic diversity analysis using restriction fragment length polymorphism (Sivaramakrishnan et al, 2002), amplified fragment length polymorphism (Panguluri et al, 2006), random amplification of polymorphic DNA (Yadav et al, 2012), microsatellite markers and DArT (Yang et al, 2006). Nevertheless, the molecular basis of most agronomic traits in pigeonpea remains unexplored due to the low level of DNA polymorphism and limited number of validated molecular markers.…”