AFLP-Based Genetic Linkage Map for the Red Flour Beetle (Tribolium castaneum)

Zhong, Daibin; Pai, Aditi; Yan, Guiyun

doi:10.1093/jhered/esh012

Cited by 21 publications

(21 citation statements)

References 60 publications

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“…Previously described markers (Zhong et al 2004;Demuth et al 2007) were used to screen for polymorphism between cSM and TIW1 parent strains. Of the 42 markers previously reported on LG3, 9 were polymorphic between the two parental strains.…”

Section: Resultsmentioning

confidence: 99%

“…The sequences of forward and reverse primers are listed in Supporting Information, Table S1. Marker polymorphism was screened using the method described previously (Zhong et al 2004 and the Li-Cor model 4300 automated DNA analyzer (Li-Cor, Lincoln, NE). The Gene ImagIR 4.33 software (LiCor) program was used to quantify allele size based on the pattern of the height of signal peaks.…”

Section: Mapping Populationsmentioning

confidence: 99%

“…The detection of PCR products and PCR conditions were described previously (Zhong et al 2004. A total of 800 individuals were genotyped in the two G 15 populations using 54 polymorphic markers (Table S1).…”

Section: Genotyping Of Ail Mapping Populationmentioning

confidence: 99%

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Fine-Scale Analysis of Parasite Resistance Genes in the Red Flour Beetle, Tribolium castaneum

et al. 2013

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Parasite infection impacts population dynamics through effects on fitness and fecundity of the individual host. In addition to the known roles of environmental factors, host susceptibility to parasites has a genetic basis that has not been well characterized. We previously mapped quantitative trait loci (QTL) for susceptibility to rat tapeworm (Hymenolepis diminuta) infection in Tribolium castaneum using dominant AFLP markers; however, the resistance genes were not identified. Here, we refined the QTL locations and increased the marker density in the QTL regions using new microsatellite markers, sequence-tagged site markers, and single-strand conformational polymorphism markers. Resistance QTL in three linkage groups (LG3, LG6, and LG8) were each mapped to intervals ,1.0 cM between two codominant markers. The effects of 21 genes in the three QTL regions were investigated by using quantitative RT-PCR analysis, and transcription profiles were obtained from the resistant TIW1 and the susceptible cSM strains. Based on transcription data, eight genes were selected for RNA interference analysis to investigate their possible roles in H. diminuta resistance, including cytochrome P450 (LOC657454) and Toll-like receptor 13 (TLR13, LOC662131). The transcription of P450 and TLR13 genes in the resistant TIW1 strains was reduced more than ninefold relative to the control. Moreover, the effects of gene knockdown of P450 and TLR13 caused resistant beetles to become susceptible to tapeworm infection, which strongly suggests an important role for each in T. castaneum resistance to H. diminuta infection. P ARASITES exert negative effects on host survivorship and reproductive success, and in turn hosts develop resistance to parasites by reducing susceptibility to infection. Despite the importance to medicine and agriculture of host resistance to parasitism, and the fact that host resistance is genetically determined, the genetics of parasite resistance in insect hosts is not well known. Infection of the red flour beetle, Tribolium castaneum, by the rat tapeworm, Hymenolepis diminuta, has been well characterized with regard to hostparasite interactions (Keymer and Anderson 1979;Zhong et al. 2003Zhong et al. , 2005. The system also represents an excellent model to study the evolutionary genetics of resistance to parasite infection, as infection can be easily controlled and monitored in the laboratory. Serving as an intermediate host, infection of Tribolium by H. diminuta occurs upon ingestion of the parasite eggs in rat feces. The eggs then hatch and develop into cysticercoids that are capable of infecting the mammalian host and amplifying when ingested, but cannot be horizontally or vertically transmitted.Previously, we used AFLP markers to identify three major quantitative trait loci (QTL) that affected T. castaneum beetle susceptibility to H. diminuta on linkage groups LG3 [hds 4(3, L1B1.69)], LG6 [hds(6, L1A16.141)], and LG8 [hds(8, L6B2.100)]. The gene action at QTL hds(3, L1B1.69) and hds(8, L6B2.100) was overdominance...

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Section: Resultsmentioning

confidence: 99%

Section: Mapping Populationsmentioning

confidence: 99%

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Fine-Scale Analysis of Parasite Resistance Genes in the Red Flour Beetle, Tribolium castaneum

et al. 2013

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“…AFLP analysis is a DNA fingerprinting technique that gives several hundred fragments in a single PCR reaction, and it is possible that some strain-specific fragments may be identified (Rieseberg et al 1999). AFLP markers have been widely used in genetic diversity and genetic mapping studies and are useful for studying genetic introgression in wild populations (Maughan et al1996;Zhu et al 1998;Yan et al 1999;Zhong et al 2004). AFLP's dominant nature is a disadvantage because each marker is represented by only two alleles, one of them being unamplifiable (null), thereby decreasing their accuracy in allele frequency estimation in comparison with codominant markers (Lynch and Milligan 1994).…”

Section: Discussionmentioning

confidence: 99%

Dynamics of Gene Introgression in the African Malaria Vector Anopheles gambiae

et al. 2006

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Anopheles gambiae is a major malaria vector in Africa and a popular model species for a variety of ecological, evolutionary, and genetic studies on vector control. Genetic manipulation of mosquito vectorial capacity is a promising new weapon for the control of malaria. However, the release of exotic transgenic mosquitoes will bring in novel alleles in addition to the parasite-inhibiting genes, which may have unknown effects on the local population. Therefore, it is necessary to develop methodologies that can be used to evaluate the spread rate of introduced genes in A. gambiae. In this study, the effects and dynamics of genetic introgression between two geographically distinct A. gambiae populations from western Kenya (Mbita) and eastern Tanzania (Ifakara) were investigated with amplified fragment length polymorphisms (AFLPs) and microsatellite markers. Microsatellites and polymorphic cDNA markers revealed a large genetic differentiation between the two populations (average F ST ¼ 0.093, P , 0.001). When the two strains were crossed in random mating between the two populations, significant differences in the rate of genetic introgression were found in the mixed populations. Allele frequencies of 18 AFLP markers (64.3%) for Mbita and of 26 markers (92.9%) for Ifakara varied significantly from F 5 to F 20 . This study provides basic information on how a mosquito release program would alter the genetic makeup of natural populations, which is critical for pilot field testing and ecological risk evaluation of transgenic mosquitoes.

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“…The gel electrophoresis was maintained at a constant power of 8 W for 5 hr at room temperature. Gels were stained with silver nitrate following the method of Bassam et al (1991) and Zhong et al (2004).…”

Section: Methodsmentioning

confidence: 99%

Amplified Fragment Length Polymorphism Mapping of Quantitative Trait Loci for Malaria Parasite Susceptibility in the Yellow Fever Mosquito Aedes aegypti

Zhong

Menge

Temu³

et al. 2006

Genetics

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The yellow fever mosquito Aedes aegypti has been the subject of extensive genetic research due to its medical importance and the ease with which it can be manipulated in the laboratory. A molecular genetic linkage map was constructed using 148 amplified fragment length polymorphism (AFLP) and six singlestrand conformation polymorphism (SSCP) markers. Eighteen AFLP primer combinations were used to genotype two reciprocal F 2 segregating populations. Each primer combination generated an average of 8.2 AFLP markers eligible for linkage mapping. The length of the integrated map was 180.9 cM, giving an average marker resolution of 1.2 cM. Composite interval mapping revealed a total of six QTL significantly affecting Plasmodium susceptibility in the two reciprocal crosses of Ae. aegypti. Two common QTL on linkage group 2 were identified in both crosses that had similar effects on the phenotype, and four QTL were unique to each cross. In one cross, the four main QTL accounted for 64% of the total phenotypic variance, and digenic epistasis explained 11.8% of the variance. In the second cross, the four main QTL explained 66% of the variance, and digenic epistasis accounted for 16% of the variance. The actions of these QTL were either dominance or underdominance. Our results indicated that at least three new QTL were mapped on chromosomes 1 and 3. The polygenic nature of susceptibility to P. gallinaceum and epistasis are important factors for significant variation within or among mosquito strains. The new map provides additional information useful for further genetic investigation, such as identification of new genes and positional cloning.

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AFLP-Based Genetic Linkage Map for the Red Flour Beetle (Tribolium castaneum)

Cited by 21 publications

References 60 publications

Fine-Scale Analysis of Parasite Resistance Genes in the Red Flour Beetle, Tribolium castaneum

Fine-Scale Analysis of Parasite Resistance Genes in the Red Flour Beetle, Tribolium castaneum

Dynamics of Gene Introgression in the African Malaria Vector Anopheles gambiae

Amplified Fragment Length Polymorphism Mapping of Quantitative Trait Loci for Malaria Parasite Susceptibility in the Yellow Fever Mosquito Aedes aegypti

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