AFLP assessment of genetic diversity among Indian Mucuna accessions

Sathyanarayana, N.; Leelambika, M.; Mahesh, S.; Jaheer, Mahammad

doi:10.1007/s12298-011-0058-6

Cited by 24 publications

(8 citation statements)

References 26 publications

(24 reference statements)

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“…All the primer combinations were attributed with resolving power (Rp) ranging from 30 to 40 (average 35.90 ± 4.95). The high values of GD, MI, and Rp are in agreement with the fairly good discriminatory potential of efficient AFLP markers (Behera et al, 2008;Blignaut et al, 2013;Kesari et al, 2010;Laurentin, 2009;Sathyanarayana et al, 2011). A significant positive correlation was noticed among MI, Rp, and GD with coefficients of determination (R 2 ) = 0.991 (MI and Rp), 0.924 (GD and MI), and 0.868 (GD and Rp).…”

Section: Resultssupporting

confidence: 69%

Amplified Fragment Length Polymorphism Fingerprinting to Identify Genetic Relatedness among Lychee Cultivars and Markers Associated with Small-seeded Cultivars

Pathak

Singh

Tuli

2014

J. Amer. Soc. Hort. Sci.

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Amplified fragment length polymorphism (AFLP) was used to assess genetic relatedness among 23 popular commercial cultivars of lychee (Litchi chinensis) and identify the markers associated with small-seeded cultivars. A combination of three primer pairs with high discriminatory ability was selected for resolving the identity of lychee cultivars. The marker system resolved the instances of synonymies and homonymies. The two cultivars, Dehrarose and Dehradun, whose names are often used interchangeably, could be discriminated by AFLP. A cultivar, Calcuttia (H), grown in the Punjab region was found genetically very similar to China (M) of Bihar region in India, suggesting that both could be the same clone. A number of cultivars with contrasting seed size were included in the study. The cultivars Bedana, Seedless Late, and China were positioned in one group, unlike the grouping previously reported based on morphological traits. The AFLP fingerprinting revealed five marker fragments exclusive to the genotypes, which bear fruit with small seed and large pulp.

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Section: Resultssupporting

confidence: 69%

Amplified Fragment Length Polymorphism Fingerprinting to Identify Genetic Relatedness among Lychee Cultivars and Markers Associated with Small-seeded Cultivars

Pathak

Singh

Tuli

2014

J. Amer. Soc. Hort. Sci.

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“…To detect the best primer combination for AFLP marker classification of germplasm, several researchers have employed GD, PIC, EMR and MI (Kim et al, 2013;Anderson et al, 1993;Sathyanarayana et al, 2011) The highly significantly correlation with GD and PIC (r 2 =0.999) in the present study agreed with the studies of Sathyanarayana et al (2011) and Kim et al (2013). In addition, Sathyanarayana et al (2011) defined MI as a measure of maker diversity. Like we also found a significant (P≤0.05) positive correlation with GD and PIC in this study.…”

Section: Discussionsupporting

confidence: 84%

Genetic Diversity and Relationship Assessment based on AFLP Analysis in Blackberry (Rubus fructicosus L.) Mutant Lines

Ryu

Kim

et al. 2014

Plant Breed. Biotech.

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This study was carried out to evaluate the genetic diversity and relationships among fifty-six blackberry (Rubus fructicosus) mutants derived from gamma-ray treatment (fifty-two lines) by analysis of Amplified fragment length polymorphism (AFLP) markers. Both cluster analysis and principal coordinate analysis (PCOORDA) were conducted in order to study the genetic diversity, using both morphological traits and AFLP makers. A total of 589 bands were amplified with an average of 58.9 bands per primer. Among them, 560 were identified to be polymorphic, with a rate of 95.08%. A showed a highly significant (P≤0.01) positive correlation with GD and PIC (r 2 =0.999). MI also showed a significant (P≤0.05) positive correlation with GD and PIC. According to the clustering analysis, all mutant lines could be classified into five categories, but the three gamma-ray treatment lines and the cross-bred line were not clustered into any groups. For the morphological traits, cluster analysis divided the blackberry germplasm into six clusters and two independent groups. In addition, the morphological dendrogram indicated an unclear pattern of division among the groups based on AFLP analysis. The findings of this study indicate that mutant lines have high genetic diversity, and can be effectively utilized as materials for the improvement of breeding.

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“…The RAPD primer RPI-B11 and the SSR primer S16 were found to be the most informative primers to discriminate the sesame genotypes. A higher value for the marker attributes is critical for considering use of the dominant (RAPD and AFLP) and codominant markers (SSR and RFLP) for classification, fingerprinting, genetic diversity analysis, gene mapping, molecular breeding, and germplasm evaluation (Sathyanarayana et al 2011). On comparative basis, we found that GD was almost similar for both RAPD (0.229) and SSR (0.230).…”

Section: Discussionmentioning

confidence: 99%

Comparative assessment of genetic diversity in Sesamum indicum L. using RAPD and SSR markers

et al. 2017

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Sesame (Sesamum indicum L.) is an ancient oilseed crop known for its nutty seeds and high-quality edible oil. It is an unexplored crop with a great economic potential. The present study deals with assessment of genetic diversity in the crop. Twenty two RAPD and 18 SSR primers were used for analysis of the 47 different sesame accessions grown in different agroclimatic zones of India. A total of 256 bands were obtained with RAPD primers, of which 191 were polymorphic. SSR primers gave 64 DNA bands, of which all of were polymorphic. The Jaccardʼs similarity coefficient of RAPD, SSR, and pooled RAPD and SSR data ranged from 0.510 to 0.885, 0.167 to 0.867, and 0.505 to 0.853, respectively. Maximum polymorphic information content was reported with SSRs (0.194) compared to RAPDs (0.186). Higher marker index was observed with RAPDs (1.426) than with SSRs (0.621). Similarly, maximum resolving power was found with RAPD (4.012) primers than with SSRs (0.884). The RAPD primer RPI-B11 and SSR primer S16 were the most informative in terms of describing genetic variability among the varieties under study. At a molecular level, the seed coat colour was distinguishable by the presence and absence of a group of marker amplicon/s. White and brown seeded varieties clustered close to each other, while black seeded varieties remained distanced from the cluster. In the present study, we found higher variability in Sesamum indicum L. using RAPD and SSR markers and these could assist in DNA finger printing, conservation of germplasm, and crop improvement.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0578-4) contains supplementary material, which is available to authorized users.

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AFLP assessment of genetic diversity among Indian Mucuna accessions

Cited by 24 publications

References 26 publications

Amplified Fragment Length Polymorphism Fingerprinting to Identify Genetic Relatedness among Lychee Cultivars and Markers Associated with Small-seeded Cultivars

Amplified Fragment Length Polymorphism Fingerprinting to Identify Genetic Relatedness among Lychee Cultivars and Markers Associated with Small-seeded Cultivars

Genetic Diversity and Relationship Assessment based on AFLP Analysis in Blackberry (Rubus fructicosus L.) Mutant Lines

Comparative assessment of genetic diversity in Sesamum indicum L. using RAPD and SSR markers

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