AFLP and SCAR markers linked to the suppressor gene (Rf) of a dominant genetic male sterility in rapeseed (Brassica napus L.)

Hong, Dengfeng; Wan, Lili; Liu, Pingwu; Yang, Guozheng; He, Qingbiao

doi:10.1007/s10681-006-9162-z

Cited by 13 publications

(12 citation statements)

References 19 publications

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“…AFLP can produce large number of candidate markers in a single experiment and it is highly reproducible [ 33 ]. However, AFLP markers are relatively costly and the method is technologically demanding, and this limits its application in breeding programs [ 34 ]. Thus, we converted an AFLP marker into a convenient and inexpensive PCR-based marker SCAR.…”

Section: Resultsmentioning

confidence: 99%

Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv.

Wang

2011

IJMS

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Eucommia ulmoides Oliv. is a dioecious species. Currently, there is no method to identify the sex during the juvenile stage that lasts a relatively long time. This study aimed to develop a sex specific Amplified Fragment Length Polymorphism (AFLP) marker for Eucommia ulmoides Oliv. Of a total of 64 AFLP primer combinations screened, primer combination E-ACA/M-CTT produced a 350 bp male-specific marker. This 350 bp AFLP marker was converted into a 247 bp Sequence Characterized Amplified Region (SCAR) marker. Results suggest that the SCAR marker can be utilized for early sexual identification in Eucommia ulmoides Oliv., and it will greatly facilitate future breeding programs.

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Section: Resultsmentioning

confidence: 99%

Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv.

Wang

2011

IJMS

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“…Linkage analysis showed that three markers, designed as SCHDA, SCHDC and SCHDF, were co‐segregating with each other. They all had the same genetic distance of 0.7 cM from the Rf gene (Hong et al. 2006).…”

Section: Resultsmentioning

confidence: 99%

“…2004a,b). Recently, the Rf gene was also tagged with six AFLP polymorphic fragments and the three nearest were converted to SCAR markers (Hong et al. 2006).…”

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confidence: 99%

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Development and characterization of SCAR markers associated with a dominant genic male sterility in rapeseed

et al. 2007

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Rs1046AB is a dominant genic male sterility (DGMS) line in rapeseed, in which the sterility has always been thought to be conditioned by the interaction of a male sterility gene (Ms) and its non-allelic restorer gene (Rf). This system provides not only a tool for assisting in recurrent selection but also a promising system for hybrid production. Based on previous studies, two amplified fragment length polymorphism markers linked with the Ms gene were converted into a dominant and a co-dominant sequence characterized amplified region (SCAR) marker, respectively. The putative linear order relationship of three dominant SCAR markers with the same genetic distance from the Rf gene, was also determined by an examination of whether the homologues of these markers are present or not in different lines carrying Rf. A bigger fragment generated by the closest marker linked to the Rf gene was observed in all lines carrying the recessive allele rf, suggesting that this marker is a co-dominant marker, which was further confirmed by nucleotide sequence comparison of these fragments. SCAR markers specific for Ms and Rf will be especially valuable in marker-assisted DGMS three-line breeding.

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“…Crossing dominant GMS plants with recessive temporary maintainer lines can produce all male sterile progenies as female parents for hybrid seed production. Numerous DNA markers closely linked with GMS genes have been developed for marker-assisted breeding and have contributed to positional cloning (Ying et al 2003;Ke et al 2004;Lu et al 2004;Ke et al 2005;Hong et al 2006;Song et al 2006;He et al 2008;Hong et al 2008;Feng et al 2009;Xu et al 2009). A total of five GMS genes were mapped on linkage groups N7, N16, N19 and A8 in B. napus (Yi et al 2006;Huang et al 2007;Lei et al 2007;Xiao et al 2008;Lu et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Genetic Analysis and Fine Mapping of a Spontaneously Mutated Male Sterility Gene inBrassica rapassp.chinensis

Lin

Lin²,

Lin

2020

G3 Genes|Genomes|Genetics

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Male sterility has been widely used in hybrid seed production in Brassica, but not in B. rapa ssp. chinensis, and genetic models of male sterility for this subspecies are unclear. We discovered a spontaneous mutant in B. rapa ssp. chinensis. A series of progeny tests indicated that male sterility in B. rapa ssp. chinensis follows a three-allele model with BrMsa, BrMsb, and BrMsc. The male sterility locus has been mapped to chromosome A07 in BC1 and F2 populations through genotyping by sequencing. Fine mapping in a total of 1,590 F2 plants narrowed the male sterility gene BrMs to a 400 kb region, with two SNP markers only 0.3 cM from the gene. Comparative gene mapping shows that the Ms gene in B. rapa ssp. pekinensis is different from the BrMs gene of B. rapa ssp. chinensis, despite that both genes are located on chromosome A07. Interestingly, the DNA sequence orthologous to a male sterile gene in Brassica napus, BnRf, is within 400 kb of the BrMs locus. The BnRf orthologs of B. rapa ssp. chinensis were sequenced, and one KASP marker (BrMs_indel) was developed for genotyping based on a 14 bp indel at intron 4. Cosegregation of male sterility and BrMs_indel genotypes in the F2 population indicated that BnRf from B. napus and BrMs from B. rapa are likely to be orthologs. The BrMs_indel marker developed in this study will be useful in marker-assisted selection for the male sterility trait.

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AFLP and SCAR markers linked to the suppressor gene (Rf) of a dominant genetic male sterility in rapeseed (Brassica napus L.)

Cited by 13 publications

References 19 publications

Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv.

Identification of a Male-Specific Amplified Fragment Length Polymorphism (AFLP) and a Sequence Characterized Amplified Region (SCAR) Marker in Eucommia ulmoides Oliv.

Development and characterization of SCAR markers associated with a dominant genic male sterility in rapeseed

Genetic Analysis and Fine Mapping of a Spontaneously Mutated Male Sterility Gene inBrassica rapassp.chinensis

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