AFLP analysis to assess genomic stability in Solanum regenerants derived from wild and cultivated species

Abstract

The cultivated potato as well as its tuber-bearing relatives are considered model plants for cell and tissue culture, and therefore for exploiting the genetic variation induced by in vitro culture. The association between molecular stability and tissue culture in different genetic backgrounds and ploidy levels has already been explored. However, it still remains to be ascertained whether somaclonal variation differs between callus-derived chromosome- doubled and undoubled regenerants. Our research aimed at investigating, through amplified fragment length polymorphism (AFLP) markers, the genetic changes in marker- banding patterns of diploid and tetraploid regenerants obtained from one clone each of Solanum bulbocastanum Dunal and S. cardiophyllum Lindl (both 2n = 2x = 24) and tetraploids from cultivated S. tuberosum L. (2n = 4x = 48). Pairwise comparisons between the banding patterns of regenerants and parents allowed detecting considerable changes associated to in vitro culture both at diploid and tetraploid level. The percentages of polymorphic bands between diploid and tetraploid regenerants were, respectively, 57 and 69% in S. bulbocastanum and 58 and 63% in S. cardiophyllum. On average, the frequencies of lost parental fragments in regenerants were significantly higher than novel bands both in S. bulbocastanum (48 vs. 22%) and S. tuberosum (36 vs. 18%) regenerants. By contrast, in S. cardiophyllum, a similar incidence of the two events was detected (32 vs. 29%). Our results revealed that structural changes after tissue culture process strongly affected the genome of the species studied, but diploid and tetraploids regenerated plants responded equally

Shoot organogenesis in three Miscanthus species and evaluation for genetic uniformity using AFLP analysis

“…AFLP analysis is commonly used to assess the genetic conformity of plants regenerated by in vitro culture (Aversano et al 2011;Mehta et al 2011) or to detect somaclonal variations (Mo et al 2009;Perez et al 2012). An analysis was performed on the rhizome-propagated plants, short-term culture (ST) in vitro-propagated plants (''vitroplants'') and long-term culture (LT) in vitro-propagated plants.…”

“…The genetic conformity of in vitro-propagated progeny can be analyzed using simple morphological observations. However, DNA marker assays, such as those for random amplified polymorphic DNA (RAPD) (Mishra et al 2011), inter simple sequence repeats (ISSR) (Liu et al 2011;Rai et al 2012) or amplified fragment length polymorphism (AFLP) (Aversano et al 2011), are efficient screens for in vitro shoot organogenesis-induced mutations because these markers are not affected by environmental factors and present more reliable and reproducible results. AFLP is an advanced technique (Saker et al 2006;Smykal et al 2007), as it combines the reliability of restriction fragment length polymorphism (RFLP) with the efficiency of RAPD.…”

A Review on the Framework for Identifying High-yielding and Stress-tolerant Oil Palm with Molecular Markers

“…Among molecular markers, AFLP produces notably more replicable marker bands. Therefore, the markers are essential in detecting in vitro genetic heterogeneity of plants [51], [52].…”

A Brief Review of Molecular Markers to Analyse Medically Important Plants

“…Many studies have analysed genetic diversity for different plant species using AFLP molecular markers [17]. Among molecular markers, AFLP produces much more reproducible bands, hence this technique is an important method to detect genetic variability of plants especially plants regenerated in vitro [18,19]. One of these studies was performed by Ebrahimi et al [20] which genetic stability of in vitro regenerated plantlets of an endangered medicinal plant, Kelussia odorotissima Mozaff.…”