AFLP analysis of Solanum phureja DNA introgressed into potato dihaploids

Abstract

Introgression of Solanum phureja DNA into S. tuberosum dihaploids was analysed by the use of amplified fragment length polymorphism (AFLP) markers. Five dihaploids, derived from crosses between S. tuberosum cv. Pentland Crown and two different S. phureja pollinators (IVP48 and EC90) were investigated by use of 17 AFLP primer pairs. Also 30 dihaploids, derived from pollination of five different S. tuberosum seed parents with S. phureja IVP101, were investigated for the presence of S. phureja‐specific markers. In total approximately 680 and 850 AFLP products were detected in the diploid S. phureja clones and in the tetraploid S. tuberosum genotypes, respectively. A total of 68 S. phureja IVP48‐specific markers were detected, while the total number of S. phureja IVP101‐specific markers was in the range of 72‐96, depending on the S. tuberosum seed parent. Introgression of DNA in the S. tuberosum cv. Pentland Crown dihaploids, after pollination with S. phureja IVP48 and S. phureja EC90, was demonstrated by the detection of 14 of 68 IPV48‐specific markers in the dihaploids. However, no DNA introgression was found in any of the 30 S. tuberosum dihaploids derived from S. phureja IVP101. Hence, S. phureja IVP101 is regarded as an excellent pollinator in the production of S. tuberosum dihaploids in potato breeding programmes because of the high yield of dihaploids per 100 berries, and because no introgression of DNA into the S. tuberosum dihaploids was evidenced.

Maximization of Markers Linked in Coupling for Tetraploid Potatoes via Monoparental Haploids

“…SNP markers specific for the S. phureja inducers occurred in almost all genotypes and on nearly all of the potato chromosomes (Table S1 ). These results are in contrast to those of Straadt and Rasmussen ( 2003 ), who observed no introgression of pollinator DNA in 30 haploid genotypes derived from crosses with S. phureja IVP101 using AFLP markers for their introgression analysis but also indicated that the introgression rate may be influenced by the tetraploid S. tuberosum seed parent. Rarely, more than a single introgression marker could be detected on the same chromosomes in the P208 population, but more often in the P809 population.…”

“…Clulow et al ( 1991 ) suggested that S. phureja chromosomes are eliminated from embryonic cells during cell divisions after the fertilization of egg cells, resulting in haploid progeny. This finding was also reported in later introgression analyses (Clulow and Rousselle-Bourgeois, 1997 ; Straadt and Rasmussen, 2003 ; Ercolano et al, 2004 ). Haploid populations derived by parthenogenesis have been used for genetic analyses (Kotch et al, 1992 ) and to identify markers that are linked to nematode resistance (Pineda et al, 1993 ); however, the number of genotypes used in the RFLP mapping approach was very small with 37 haploid individuals.…”

Genomic Outcomes of Haploid Induction Crosses in Potato (Solanum tuberosumL.)

“…In potato, evidence in support of parthenogenesis has been reported (Wangenheim et al 1960;Montelongo-Escobedo and Rowe 1969;Peloquin et al 1996) . At the same time, genome elimination is supported by the detection of genetic markers from the haploid inducer in euploids and aneuploids arising from haploid induction crosses (Clulow et al 1991(Clulow et al , 1993Waugh et al 1992;Wilkinson et al 1995;Allainguillaume et al 1997;Clulow and Rousselle-Bourgeois 1997;Straadt and Rasmussen 2003;Ercolano et al 2004) . Whole genome sequencing provides a more informative and reliable method to assess the genetic contribution of the haploid inducer.…”

“…This alternative hypothesis is supported by the presence of inducer-specific AFLP, RFLP, or isozyme markers in presumably dihaploid progeny. Often, progeny exhibiting genetic markers from the haploid inducer are also aneuploid, suggesting inheritance of an entire chromosome from the haploid inducer (Clulow et al 1991(Clulow et al , 1993Waugh et al 1992;Wilkinson et al 1995;Allainguillaume et al 1997;Clulow and Rousselle-Bourgeois 1997;Straadt and Rasmussen 2003;Ercolano et al 2004) . These results are consistent with haploid induction crosses in maize (Riera-Lizarazu et al 1996;Zhao et al 2013) , Arabidopsis (Maheshwari et al 2015;Tan et al 2015;Kuppu et al 2015) , and oat-maize hybrids (Riera-Lizarazu et al 1996) in which one or more haploid inducer chromosomes persist in otherwise haploid plants.…”

Molecular description and similarity relationships among native germplasm potatoes (Solanum tuberosum ssp. tuberosum L.) using morphological data and AFLP markers

“…The AFLP technique (Vos et al 1995) generally produces between 50 and 100 scorable fragments per polimerase chain reaction (PCR) reaction (Maughan et al 1996). This technique has been widely used in various crops, including cultivated potatoes (Powell et al 1996;Milbourne et al 1997;McGregor et al 2000;Avrova et al 2002;Straadt and Rasmussen, 2003;Furini and Wunder, 2004) and their wild relatives (Kardolus et al 1998). In the last years, the AFLP technology was used to differentiate genotypes used as ancestors in potato genetic improvement programs.…”