AFLP analysis for examining genetic differences in cultivated strains and their single-spore isolates and for confirming successful crosses in Agaricus blazei

Mahmud, Md. Arif; Kitaura, Hiroyuki; Fukuda, Masaki; Yamada, Akiyoshi

doi:10.1007/s10267-007-0372-6

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…Among many available DNA-based markers, AFLP markers are more reliable, yield a large number of markers per reaction, are cost-effective and have wide genome coverage (Prashanth et al, 2002;Hansen et al, 1999). AFLP markers have been successfully used, in the last decade, as a satisfactory alternative as well as compliment to morphological data in a variety of plant and tree species (Basha et al, 2007;Mahmud et al, 2007;Sreekumar et al, 2007). The purpose of the present study was to estimate the level of genetic diversity and to assess genetic relationships among 50 jackfruit accessions, collected from different parts of south India, using AFLP markers.…”

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confidence: 99%

Evaluation of genetic diversity in jackfruit (Artocarpus heterophyllus Lam.) based on amplified fragment length polymorphism markers

Shyamalamma

Chandra²,

Hegde³

et al. 2008

Genet. Mol. Res.

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ABStrACt. Artocarpus heterophyllus Lam., commonly called jackfruit, is a medium-sized evergreen tree that bears high yields of the largest known edible fruit. Yet, it has been little explored commercially due to wide variation in fruit quality. The genetic diversity and genetic relatedness of 50 jackfruit accessions were studied using amplified fragment length polymorphism markers. Of 16 primer pairs evaluated, eight were selected for screening of genotypes based on the number and quality of polymorphic fragments produced. These primer combinations produced 5976 bands, 1267 (22%) of which were polymorphic. Among the jackfruit accessions, the similarity coefficient ranged from 0.137 to 0.978; the accessions also shared a large number of monomorphic fragments (78%). Cluster analysis and principal component analysis grouped all jackfruit genotypes into three major clusters. Cluster I included the genotypes grown in a jackfruit region of Karnataka, called Tamaka, with very dry conditions; cluster II contained the genotypes collected from locations having medium to heavy rainfall in Karnataka; cluster III grouped the genotypes in distant locations with different environmental conditions. Strong coincidence of these amplified fragment length polymorphism-based groupings with geographical localities as well as morphological characters was observed. We found moderate genetic diversity in these jackfruit accessions. This information should be useful for tree breeding programs, as part of our effort to popularize jackfruit as a commercial crop.

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confidence: 99%

Evaluation of genetic diversity in jackfruit (Artocarpus heterophyllus Lam.) based on amplified fragment length polymorphism markers

Shyamalamma

Chandra²,

Hegde³

et al. 2008

Genet. Mol. Res.

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“…The development of molecular markers would enrich our toolbox for studying the biology of this mushroom and developing genetic approaches. To date, marker techniques described on A. subrufescens are limited to random amplification of polymorphic DNA (RAPD; Colauto et al ., ; Fukuda et al ., ; Neves et al ., ; Tomizawa et al ., ) and amplified fragment length polymorphism (AFLP; Mahmud et al ., ). These techniques generate anonymous and dominant markers and thus are in appropriate for some genetic applications (Allan & Max, ).…”

Section: Introductionmentioning

confidence: 97%

“…However, the expansion of A. subrufescens ‐related technologies appears to be limited (Largeteau et al ., ). First, few commercial cultivars are currently available and these showed high genetic homogeneity (Colauto et al ., ; Fukuda et al ., ; Mahmud et al ., ; Tomizawa et al ., ) raising the issue of crop health and the economic risks related to disease susceptibility of a monocrop. Secondly, although an extensive literature is available on its pharmacological interest (Firenzuoli et al ., ; Oliveira et al ., ; Wisitrassameewong et al ., ), studies on its ecology, reproductive biology, biodiversity and genetics are scarce (Kerrigan, ; Largeteau et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Development of polymorphic microsatellite markers issued from pyrosequencing technology for the medicinal mushroom Agaricus subrufescens

Foulongne-Oriol

Spataro

Moinard

et al. 2012

FEMS Microbiol Lett

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The recently described procedure of microsatellite-enriched library pyrosequencing was used to isolate microsatellite loci in the gourmet and medicinal mushroom Agaricus subrufescens. Three hundred and five candidate loci containing at least one simple sequence repeats (SSR) locus and for which primers design was successful, were obtained. From a subset of 95 loci, 35 operational and polymorphic SSR markers were developed and characterized on a sample of 14 A. subrufescens genotypes from diverse origins. These SubSSR markers each displayed from two to 10 alleles with an average of 4.66 alleles per locus. The observed heterozygosity ranged from 0 to 0.71. Several multiplex combinations can be set up, making it possible to genotype up to six markers easily and simultaneously. Cross-amplification in some closely congeneric species was successful for a subset of loci. The 35 microsatellite markers developed here provide a highly valuable molecular tool to study genetic diversity and reproductive biology of A. subrufescens.

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The medicinal Agaricus mushroom cultivated in Brazil: biology, cultivation and non-medicinal valorisation

Largeteau

Llarena‐Hernández

Régnault-Roger

et al. 2011

Appl Microbiol Biotechnol

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Sun mushroom is a cultivated mushroom extensively studied for its medicinal properties for several years and literature abounds on the topic. Besides, agronomical aspects were investigated in Brazil, the country the mushroom comes from, and some studies focus on the biology of the fungus. This review aimed to present an overview of the non-medicinal knowledge on the mushroom. Areas of commercial production and marketing trends are presented. Its specific fragrance, taste, nutritional value and potential use of extracts as food additives are compared to those of the most cultivated fungi and laboratory models. The interest of the mushroom for lignocellulosic enzyme production and source of biomolecules for the control of plant pathogens are shown. Investigation of genetic variability among cultivars is reported. Growing and storage of mycelium, as well as cultivation conditions (substrate and casing generally based on local products; indoor and outdoor cultivation; diseases and disorders) are described and compared to knowledge on Agaricus bisporus.

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AFLP analysis for examining genetic differences in cultivated strains and their single-spore isolates and for confirming successful crosses in Agaricus blazei

Cited by 6 publications

References 25 publications

Evaluation of genetic diversity in jackfruit (Artocarpus heterophyllus Lam.) based on amplified fragment length polymorphism markers

Evaluation of genetic diversity in jackfruit (Artocarpus heterophyllus Lam.) based on amplified fragment length polymorphism markers

Development of polymorphic microsatellite markers issued from pyrosequencing technology for the medicinal mushroom Agaricus subrufescens

The medicinal Agaricus mushroom cultivated in Brazil: biology, cultivation and non-medicinal valorisation

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