Aflatoxins B1, B2 and G1 modulate cytokine secretion and cell surface marker expression in J774A.1 murine macrophages

Bruneau, J.; Stack, Edwina; O’Kennedy, Richard; Loscher, Christine E.

doi:10.1016/j.tiv.2012.03.003

Cited by 35 publications

(32 citation statements)

References 25 publications

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“…Researchers have even used higher doses of AFs [21]. Although our knowledge on interaction(s) of AFs effects in animals, especially food/dairy-producing ruminants, is Journal of Food Quality limited, nevertheless it is worth mentioning the point of intake of AFB 1 with the diet and its transfer to milk in high yielding lactating cows.…”

Section: Discussionmentioning

confidence: 99%

Seasonally Feed-Related Aflatoxins B₁ and M₁ Spread in Semiarid Industrial Dairy Herd and Its Deteriorating Impacts on Food and Immunity

Mozafari

Mohsenzadeh

Mehrzad

2017

Journal of Food Quality

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To comparatively determine the levels of aflatoxin (AF) B 1 in feedstuffs and of AFM 1 in milk from semiarid industrial cattle farms in northeastern Iran during four seasons and to elucidate the effects of mixed AFB 1 and AFM 1 on bovine granulocytes, 72 feedstuffs (concentrate, silage, and totally mixed ration (TMR)) and 200 bulk milk samples were simultaneously collected for ELISA-based AFs detection. Isolated blood and milk neutrophils ( = 8/treatment) were also preincubated with mix of 10 ng/ml AFB 1 and 10 ng/ml AFM 1 for 12 h; the impact was assessed on neutrophils functions. AFB 1 levels in feedstuffs averaged 28 g/kg (4-127 g/kg), with TMR maximal (38 ± 6.3 g/kg), concentrate (32 ± 6.5 g/kg), and silage (16 ± 1.5 g/kg). The levels of AFB 1 and AFM 1 in feedstuffs and milk averaged 42 ± 9.3, 27 ± 2.8, 26 ± 4.1, and 18.5 ± 2.8 g/kg and 85 ± 7.3, 62 ± 6.1, 46 ± 6.2, and 41 ± 6.5 ppb g/kg in winter (maximal), autumn, spring, and summer, respectively. Mix of AFB 1 and AFM 1 weakened various functions of granulocytes. It adds new reason why during winter semiarid raised food-producing animals show more immune-incompetence.

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Section: Discussionmentioning

confidence: 99%

Seasonally Feed-Related Aflatoxins B₁ and M₁ Spread in Semiarid Industrial Dairy Herd and Its Deteriorating Impacts on Food and Immunity

Mozafari

Mohsenzadeh

Mehrzad

2017

Journal of Food Quality

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“…These results confirm the pro-inflammatory role of OTA by itself, and demonstrate the impaired capability of OTA-treated macrophages to respond properly to noxious stimuli, such as LPS, mimicking the environmental co-exposure to both compounds. Bruneau et al (2012) investigated the effect of AFB1, AFB2 and AFG1 exposure, alone and in combination, on the secretion of key pro-and anti-inflammatory cytokines from the murine macrophage cell line, J774A.1. Exposure of macrophages to low doses of aflatoxin (0.01 or 0.1 ng/mL) resulted in a statistically significant change in the secretion of a number of cytokines following stimulation with LPS.…”

Section: Discussionmentioning

confidence: 99%

“…Immunosuppression is viewed as one of the most significant effects of mycotoxin exposure and it has a major economic impact. Studies have already highlighted the immunosuppressive nature of several mycotoxins such as aflatoxins, trichothecenes, ochratoxin A and zearalenone (Ferrante et al, 2002;Sharma et al, 2004;Ferrante et al, 2008;Ubagai et al, 2008;Marzocco et al, 2009;Bianco et al, 2012;Bruneau et al, 2012;Alassane-Kpembi et al, 2013;Jia et al, 2014). Immunostimulation and immunosuppression can occur with the same toxin depending on the exposure conditions, including dosage and time.…”

Section: Introductionmentioning

confidence: 99%

“…Additive and synergistic effects of mycotoxins were demonstrated for various combinations of mycotoxins, such as ochrations, aflatoxins and ZEN, (Grenier and Oswald, 2011;Halbin et al, 2013;Lei et al, 2013). In vitro studies have shown that mycotoxin combinations can act additively, synergistically and even in some cases antagonistically (Bruneau et al, 2012;Clarke et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…8, No. 2;2016 production of the pro-inflammatory cytokines and to ensure regulation of the immune response (Bruneau et al, 2012). Although these cytokines have important roles in host defence, their over-or under-expression can lead to problems such as inflammatory diseases.…”

Section: Introductionmentioning

confidence: 99%

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Patulin, Deoxynivalenol, Zearalenone and T-2 Toxin Affect Viability and Modulate Cytokine Secretion in J774A.1 Murine Macrophages

Loftus¹,

Kijanka²,

O’Kennedy³

et al. 2016

IJC

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Mycotoxins are secondary fungal metabolites, which occur in food and feed. They have detrimental effects on the health of humans and animals, and they are known to cause immunosuppression. In this study the effect of patulin, deoxynivalenol (DON), zearalenone (ZEN) and T-2 toxin exposure on the viability and the secretion of key pro-and anti-inflammatory cytokines from the murine macrophage cell line, J774A.1, was investigated. Exposure of macrophages to high doses of ZEN (100,000 pg/mL) and T-2 toxin (10,000 and 100,000 pg/mL) resulted in a significant decrease (P < 0.05 and P < 0.01) in cell viability. Exposure of macrophages to these mycotoxins resulted in a dose-dependent modulation of cytokine secretion. Specifically, exposure to low doses of patulin (0.001, 0.1 and 1 pg/mL) resulted in a statistically significant decrease in the secretion of the pro-inflammatory cytokines interleukin (IL) 6 (IL-6) and tumor necrosis factor alpha (TNF-α), following stimulation with lipopolysaccharide (LPS), a component of Gram-negative bacterial cell walls. Treatment with low doses of DON (0.001 pg/mL) and ZEN (0.001 and 0.01 pg/mL) significantly decreased (P < 0.01) the secretion of the pro-inflammatory cytokine IL-12p40, while several doses of T-2 toxin (0.001, 0.01, 0.1, 1 and 100 pg/mL) caused a significant decrease the expression of IL-6. Each of the mycotoxins also significantly increased the production of the anti-inflammatory cytokine IL-10, both before and after LPS stimulation. This data provides further insight into the mechanisms by which mycotoxins modulate the host immune response to exert their immunosuppressive activity.

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Antioxidative properties, phenolic compounds, and in vitro protective efficacy of multi‐herbal hydro‐alcoholic extracts of ginger, turmeric, and thyme against the toxicity of aflatoxin B₁ on mouse macrophage RAW264.7 cell line

Nazdar,

Imani,

Abtahi Froushani

et al. 2024

Food Science & Nutrition

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Aflatoxin B1 (AFB1), the most potent toxic and carcinogenic secondary fungal metabolite, has frequently been reported in food/feed. Nowadays, herbal extracts are considered safe dietary additives to reduce the toxicity of such compounds. The protective capability of various combinations of hydro‐alcoholic extracts (HAEs) of ginger, turmeric, and Shirazi thyme, against the toxicity of AFB1 on the RAW264.7 cell line was investigated. The RAW264.7 cells were exposed to six different concentrations of AFB1 (0.09, 0.18, 0.37, 0.75, 1.5, and 3 μg mL−1) for 48 h to determine the IC50 of AFB1. AFB1 was estimated to have an IC50 of 1.5 μg mL−1 for RAW264.7 cells. Then, the cells were simultaneously incubated with 1.5 μg mL−1 AFB1 and the HAEs for 24 h. The HAEs significantly reduced the toxicity of AFB1 in RAW264.7 cells. HAE of Shirazi thyme showed the highest amount of total phenol content (TPC) and the highest DPPH• activity. In addition, a combination of ginger, turmeric, and Shirazi thyme extract showed the highest antioxidant activity. Rutin, quercetin, and apigenin were the main phenolic components of ginger HAE. A significantly positive correlation was observed between TPC of hydro‐alcoholic extract with ferric reducing antioxidant power (FRAP) and 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) values. Consequently, the simultaneous consumption of such extracts is recommended to protect the cells against dietary toxins.

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Aflatoxins B1, B2 and G1 modulate cytokine secretion and cell surface marker expression in J774A.1 murine macrophages

Cited by 35 publications

References 25 publications

Seasonally Feed-Related Aflatoxins B₁ and M₁ Spread in Semiarid Industrial Dairy Herd and Its Deteriorating Impacts on Food and Immunity

Seasonally Feed-Related Aflatoxins B₁ and M₁ Spread in Semiarid Industrial Dairy Herd and Its Deteriorating Impacts on Food and Immunity

Patulin, Deoxynivalenol, Zearalenone and T-2 Toxin Affect Viability and Modulate Cytokine Secretion in J774A.1 Murine Macrophages

Antioxidative properties, phenolic compounds, and in vitro protective efficacy of multi‐herbal hydro‐alcoholic extracts of ginger, turmeric, and thyme against the toxicity of aflatoxin B₁ on mouse macrophage RAW264.7 cell line

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Aflatoxins B1, B2 and G1 modulate cytokine secretion and cell surface marker expression in J774A.1 murine macrophages

Cited by 35 publications

References 25 publications

Seasonally Feed-Related Aflatoxins B1 and M1 Spread in Semiarid Industrial Dairy Herd and Its Deteriorating Impacts on Food and Immunity

Seasonally Feed-Related Aflatoxins B1 and M1 Spread in Semiarid Industrial Dairy Herd and Its Deteriorating Impacts on Food and Immunity

Patulin, Deoxynivalenol, Zearalenone and T-2 Toxin Affect Viability and Modulate Cytokine Secretion in J774A.1 Murine Macrophages

Antioxidative properties, phenolic compounds, and in vitro protective efficacy of multi‐herbal hydro‐alcoholic extracts of ginger, turmeric, and thyme against the toxicity of aflatoxin B1 on mouse macrophage RAW264.7 cell line

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Product

Resources

About

Seasonally Feed-Related Aflatoxins B₁ and M₁ Spread in Semiarid Industrial Dairy Herd and Its Deteriorating Impacts on Food and Immunity

Seasonally Feed-Related Aflatoxins B₁ and M₁ Spread in Semiarid Industrial Dairy Herd and Its Deteriorating Impacts on Food and Immunity

Antioxidative properties, phenolic compounds, and in vitro protective efficacy of multi‐herbal hydro‐alcoholic extracts of ginger, turmeric, and thyme against the toxicity of aflatoxin B₁ on mouse macrophage RAW264.7 cell line