Aflatoxins and ochratoxin A in stored barley grain in Spain and impact of PCR-based strategies to assess the occurrence of aflatoxigenic and ochratoxigenic Aspergillus spp.

Mateo, Eva M.; Gil-Serna, Jéssica; Patiño, Belén; Jiménez, Misericordia

doi:10.1016/j.ijfoodmicro.2011.06.006

Cited by 58 publications

(31 citation statements)

References 65 publications

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“…On the basis of toxicity aflatoxins are categorized as B 1 > G 1 > B 2 > G 2 . AFB 1 has been detected in a number of food commodities including cereals such as maize (Karami-Osboo et al, 2012), rice (Reddy et al, 2009) and barley (Mateo et al, 2011). Moreover, AFB 1 has also been found in poultry (Bibin Becha and Devi, 2013) and other animal feeds (Britzi et al, 2013;Bagheri et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Seasonal variation of aflatoxin B<sub>1</sub> content in dairy feed

Ismail¹,

Riaz²,

Akhtar³

et al. 2017

J. Anim. Feed Sci.

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Section: Introductionmentioning

confidence: 99%

Seasonal variation of aflatoxin B<sub>1</sub> content in dairy feed

Ismail¹,

Riaz²,

Akhtar³

et al. 2017

J. Anim. Feed Sci.

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“…The separation and detection processes were modified from Mateo et al (2011). Detection method for AFB1 used fluorescence-based detection in collaboration with a separation process, high-performance liquid chromatography (HPLC).…”

Section: B1 Extraction Purification and Determinationmentioning

confidence: 99%

The role of Aspergillus oryzae KKB4 in reducing and detoxifying aflatoxin B1 applied in moist-heated corn

Carolina¹,

Setyabudi²,

Sardjono³

2017

MJM

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Aims:The growth and metabolic activity of Aspergillus oryzae KKB4 in AFB1-contaminated corn and later coincided with AFB1 reduction and detoxification were investigated. Methodology and results:The decreasing of AFB1 amount by A. oryzae KKB4 could be clearly observed if the initial AFB1 concentration in corn was increased. Thus, moist-heated corn was artificially inoculated with Aspergillus flavus FNCC 62C7 to increase AFB1 content. AFB1-contaminated corn was applied as solid substrate and then inoculated with A. oryzae KKB4. During fermentation periods, the growth, metabolic activity, and AFB1 decline were investigated by glucosamine content, water content loss, and AFB1 concentration, respectively. The maximum growth was occurred in 4 th day at 1.499 ± 0.028 g glucosamine/ 100 g dry matter. The metabolic activity was going on up to the end of fermentation days, as shown as water content loss at 0.175 ± 0.007 g/g dry matter. In accordance with the growth and metabolic activity, the amount of AFB1 reduction was 37.04 ng AFB1/g dry matter during 5 days fermentation. According to toxicity analysis, it was found that the residues of AFB1 were not toxic to Bacillus megaterium cells. Conclusion, significance and impact of study: A. oryzae KKB4 is able to be applied in solid substrates as AFB1 reduction and detoxification agent. These lucrative effects are also important in relation with food and feed safety.

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“…An indirect approximation for the determination of putative OTA presence in grapes is to determine the quantity of the fungi in grapes and thus use this value as an indicative risk factor. Mateo et al (2011) reported the discrepancy between the expected numbers of fungal cfu/g and the high levels of naturally contaminated barley that occurred from PCR assays and suggested that this difference could be attributed to the existence of a high number of non-viable population (spores and/or hyphae) in the samples. Thus, the aim of the present study is to determine the presence and load of A. carbonarius in wine grapes of the most widely planted grape variety in Greece, using a real time PCR assay.…”

Section: Introductionmentioning

confidence: 99%