Aflatoxin B1 exposure triggers hepatic lipotoxicity via p53 and perilipin 2 interaction-mediated mitochondria-lipid droplet contacts: An in vitro and in vivo assessment

Downregulation of PLIN2 in human dermal fibroblasts impairs mitochondrial function in an age‐dependent fashion and induces cell senescence via GDF15

“…Moreover, it has been recently shown that under stress conditions, mitochondrial p53 can interact with PLIN2 and this interaction can mediate an increase in LDs-mitochondria contacts, leading to lipid accumulation (Che et al, 2023). Mitochondrial dysfunction and cell senescence are intimately linked, and the former has been proven to cause the latter in a series of experimental models (Guan et al, 2019;Miwa et al, 2022;Wiley et al, 2016).…”

“…Another group showed that downregulation of PLIN2 in pancreatic β cells obtained from mice on high‐fat diet affects insulin secretion, decreases OXPHOS subunit expression, and reduces mitochondrial respiration (Mishra et al., 2021 ). Moreover, it has been recently shown that under stress conditions, mitochondrial p53 can interact with PLIN2 and this interaction can mediate an increase in LDs–mitochondria contacts, leading to lipid accumulation (Che et al., 2023 ). Mitochondrial dysfunction and cell senescence are intimately linked, and the former has been proven to cause the latter in a series of experimental models (Guan et al., 2019 ; Miwa et al., 2022 ; Wiley et al., 2016 ).…”

Fluorescent Probe as Dual-Organelle Localizer Through Differential Proton Gradients Between Lipid Droplets and Mitochondria

“…Lipid droplets (LDs) are dynamic organelles found in most cell types, from adipocytes to cancer cells, serve as a site of triglyceride and cholesterol storage, and are characterized by a monophospholipid membrane surrounding a hydrophobic lipid core . Crucially, the role of LDs in redox homeostasis and cellular stress has been correlated to cancer as an energy storage reservoir . This feature opens up the opportunity to connect both LDs and mitochondrial dynamics in a reliable scenario to monitor subcellular health status.…”

“… 5 Crucially, the role of LDs in redox homeostasis and cellular stress has been correlated to cancer as an energy storage reservoir. 6 This feature opens up the opportunity to connect both LDs and mitochondrial dynamics in a reliable scenario to monitor subcellular health status.…”

Editorial: Lipid droplets and mitochondria in metabolic diseases

“…Recent studies have identified key proteins that play a role in the LD–mitochondria interaction. For instance, in the liver, exposure to aflatoxin B1 (AFB1) can cause liver injury through the interaction between mitochondrial p53 (mito-p53) and the LD-associated protein perilipin 2 (PLIN2), which impeded the lysosome-dependent lipophagy ( Che et al, 2023 ). Overexpression of PLIN5 has been shown to promote LD formation and enhance LD–mitochondria contact, leading to reduced cellular ROS levels and upregulation of mitochondrial function-related genes, such as COX (cytochrome c oxidase subunit IV) and CS (citrate synthase), in HepG2 cells ( Tan et al, 2019 ).…”