Aflatoxin B1 and fumonisin B1 affect the oxidative status of bovine peripheral blood mononuclear cells

Bernabucci, U.; Colavecchia, L.; Danieli, Pier Paolo; Basiricò, Loredana; Lacetera, Nicola; Nardone, A.; Ronchi, Bruno

doi:10.1016/j.tiv.2011.01.009

Cited by 46 publications

(36 citation statements)

References 62 publications

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“…Although the expression of major AF-metabolizing enzymes in intestinal epithelial cells is yet to be explored, detoxification of AF by these cells is minimal, and these highly carcinogenic AF can easily reach the blood stream. Thereafter, the AF are converted to highly oxidative epoxides that bind to proteins, DNA, and/or RNA, causing oxidative stress and disturbing normal cellular processes (Bernabucci et al, 2011;Mehrzad et al, 2011). Indeed, the toxicity of AF not only depends on their intact form but also on their biotransformation to reactive hydroxylated derivatives (Ayed-Boussema et al, 2012;Tulayakul et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Aflatoxins of type B and G affect porcine dendritic cell maturationin vitro

Mehrzad

Devriendt

Baert

et al. 2014

Journal of Immunotoxicology

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The toxic effects of highly carcinogenic mycotoxins, especially aflatoxins (AF), on key antigenpresenting cells, such as dendritic cells (DC), are largely unknown. To elucidate the effect of AF on DC function, porcine monocyte-derived DC (MoDC) were treated with a mixture of several AF (i.e., AFB 1 , AFB 2 , AFG 1 , and AFG 2 ) and the phagocytic capacity, the membrane expression level of several DC activation markers, the T-cell proliferation-inducing capacity, and the cytokine secretion pattern were assessed. As compared to untreated MoDC, AF significantly up-regulated the expression of the co-stimulatory molecules CD25 and CD80/86. However, the phagocytic activity of MoDC was not affected by AF treatment. While the cytokine secretion pattern of AF-treated MoDC was similar to control MoDC, the T-cell proliferation-inducing capacity of MoDC was increased upon aflatoxin treatment. The results indicate that a mixture of naturally occurring AF enhances the antigen-presenting capacity of DC, which could explain the observed immunotoxicity of AF by breaking down tolerance and further emphasizes the need to reduce the admissible level of AF in agricultural commodities.

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Section: Discussionmentioning

confidence: 99%

Aflatoxins of type B and G affect porcine dendritic cell maturationin vitro

Mehrzad

Devriendt

Baert

et al. 2014

Journal of Immunotoxicology

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“…Mycotoxins can cause cytotoxicity; however, aflatoxins' exact mechanism is not fully recognized [9]. One of the probable mechanisms…”

Section: Introductionmentioning

confidence: 99%

“…Volume 11, No 2, March-April 2017; http://www.ijt.ir of aflatoxins in cellular toxicity is causing oxidative stress attributed to the imbalance between oxidants and antioxidants [9][10][11][12]. The increased generation of free radicals and an increase in lipid peroxidation may enhance aflatoxin production by toxigenic strains [13].…”

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confidence: 99%

Evaluation of Nephrotoxic Effects of Aflatoxins on Common Carp (Cyprinus carpio)

Taheri

Banaee

Haghi

et al. 2017

IJT

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“…Consistent with this study, Taranu et al (2010), in experiments with human and porcine lymphocytes, also found considerable reduction of lymphocyte cellular proliferation at doses 1 and 10 μg/mL of AFB 1 . Contradictory results were reported by Bernabucci et al (2011), who found increased viability of bovine lymphocytes in the presence of 20 μg/mL of AFB 1 on days 2 and 7 of incubation.…”

Section: Discussionmentioning

confidence: 70%

In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens

Zimmermann

Machado

Cadoná

et al. 2014

Rev. Bras. Cienc. Avic.

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The aim of the present work was to study the in-vitro cytotoxic effects of different concentrations of aflatoxin B 1 (AFB 1 ) on broiler lymphocytes. Lymphocyte-rich mononuclear cells were separated by Ficoll-Histopaque density and cultured in 96-wellplates containing the evaluated AFB 1 concentrations in 5% CO 2 atmosphere at 39°C. Thereafter, MTT, PicoGreen, and reactive oxygen species assays were performed. Cell viability decreased in the presence of 10 µg/mL AFB 1 at 48 h (p < 0.05) and of 10 and 20 µg/mL AFB 1 at 72 h (p < 0.01 and p < 0.001, respectively) when compared to the control (0 µg/mL). However, a dose-dependent increase in the cell-free DNA at 24 h was observed at 1, 10 and 20 µg/mL (p < 0.001). ROS formation significantly increased at 24 h at all concentrations (p < 0.001). The in-vitro results demonstrate that AFB 1 is cytotoxic and causes biomolecular oxidative damage in broiler lymphocytes.

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Aflatoxin B1 and fumonisin B1 affect the oxidative status of bovine peripheral blood mononuclear cells

Cited by 46 publications

References 62 publications

Aflatoxins of type B and G affect porcine dendritic cell maturationin vitro

Aflatoxins of type B and G affect porcine dendritic cell maturationin vitro

Evaluation of Nephrotoxic Effects of Aflatoxins on Common Carp (Cyprinus carpio)

In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens

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