Aflatoxin B1 alters meat quality associated with oxidative stress, inflammation, and gut-microbiota in sheep

Cao, Qi; Lin, Luxi; Xu, Tingting; Lü, Ying; Zhang, Chaodong; Ke, Yanxiong; Huang, Shucheng; Dong, Hui; Jian, Fuchun

doi:10.1016/j.ecoenv.2021.112754

Cited by 37 publications

(26 citation statements)

References 40 publications

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“…Consistent with our results, some studies have reported that AFB1 exposure induced oxidative stress and inflammation by increasing the ratio of Firmicutes/ Bacteroidetes, but MT pretreatment alleviated liver inflammation, obesity, and lipid metabolism depending on decreasing the Firmicutes/Bacteroidetes ratio. 8,10,12,21 Numerous studies have reported that increases of Desulfovibrio, Clostridium, Helicobacter, and Escherichia abundance were positively associated with the increase of LPS levels in serum, liver, and feces. 44,45 Similarly, our results also observed that AFB1 exposure increased the abundance of LPS-producing related bacteria, such as Desulfovibrio and Clostridiales, while MT pretreatment restored these changes.…”

Section: Discussionmentioning

confidence: 99%

“…15 Studies have confirmed that AFB1 could increase intestinal barrier permeability, induce intestinal barrier dysfunction, and disturb the community structure of gut microbiota and metabolic pathways. [16][17][18][19][20][21] Multiple studies have demonstrated that the gut and the liver are not only associated with in physiological structure, but also closely associated with physiological structure. 15,22,23 Additionally, numerous studies have reported that some beneficial chemicals could have protective effects on liver or kidney injury by regulating gut microbiota.…”

Section: Introductionmentioning

confidence: 99%

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Melatonin mitigates aflatoxin B1‐induced liver injury via modulation of gut microbiota/intestinal FXR/liver TLR4 signaling axis in mice

Liu

Kang

Mao

et al. 2022

Journal of Pineal Research

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Melatonin mitigates aflatoxin B1‐induced liver injury via modulation of gut microbiota/intestinal FXR/liver TLR4 signaling axis in mice

Liu

Kang

Mao

et al. 2022

Journal of Pineal Research

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“…This maybe due to a high-energy diet containing a large amount of non-fibrous carbohydrates, which can be rapidly fermented by rumen microorganisms. Nonetheless, the composition of rumen microbiota determines rumen fermentation types (e.g., amylolytic, proteolytic, and cellulolytic), and the products of fermentation impact the traits of the host meat ( 60 ). Firmicutes and Bacteroidetes were the primary phyla altered by dietary energy levels in this study, and they were highly correlated with carbohydrate metabolism, which is consistent with previous findings ( 61 , 62 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolomics approach reveals high energy diet improves the quality and enhances the flavor of black Tibetan sheep meat by altering the composition of rumen microbiota

et al. 2022

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This study aims to determine the impact of dietary energy levels on rumen microbial composition and its relationship to the quality of Black Tibetan sheep meat by applying metabolomics and Pearson's correlation analyses. For this purpose, UHPLC-QTOF-MS was used to identify the metabolome, whereas 16S rDNA sequencing was used to detect the rumen microbiota. Eventually, we observed that the high energy diet group (HS) improved the carcass quality of Black Tibetan sheep and fat deposition in the longissimus lumborum (LL) compared to the medium energy diet group (MS). However, HS considerably increased the texture, water holding capacity (WHC), and volatile flavor of the LL when compared to that of MS and the low energy diet group (LS). Metabolomics and correlation analyses revealed that dietary energy levels mainly affected the metabolism of carbohydrates and lipids of the LL, which consequently influenced the content of volatile flavor compounds (VOCs) and fats. Furthermore, HS increased the abundance of Quinella, Ruminococcus 2, (Eubacterium) coprostanoligenes, and Succinivibrionaceae UCG-001, all of which participate in the carbohydrate metabolism in rumen and thus influence the metabolite levels (stachyose, isomaltose, etc.) in the LL. Overall, a high-energy diet is desirable for the production of Black Tibetan sheep mutton because it improves the mouthfeel and flavor of meat by altering the composition of rumen microbiota, which influences the metabolism in the LL.

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“…Pigs exposed to aflatoxin-contaminated feed are highly susceptible to secondary infections [ 10 ]. AFB 1 can also negatively impact meat quality due to associated oxidative stress, inflammation, and the dysregulation of gut microbiota and dysbiosis in animals [ 11 , 12 ]. Various physical, chemical, and biological approaches to preventing AFB 1 contamination have been developed [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Scale CRISPR Knockout Screening Identifies BACH1 as a Key Regulator of Aflatoxin B1-Induced Oxidative Damage

Zhang

Zhao

et al. 2022

Antioxidants

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Aflatoxin B1 (AFB1) is amongst the mycotoxins commonly affecting human and animal health, raising global food safety and control concerns. The mechanisms underlying AFB1 toxicity are poorly understood. Moreover, antidotes against AFB1 are lacking. Genome-wide CRISPR/Cas9 knockout screening in porcine kidney cells identified the transcription factor BTB and CNC homolog 1 (BACH1) as a gene required for AFB1 toxicity. The inhibition of BACH1 expression in porcine kidney cells and human hepatoma cells resulted in increased resistance to AFB1. BACH1 depletion attenuates AFB1-induced oxidative damage via the upregulation of antioxidant genes. Subsequently, virtual structural screening identified the small molecule 1-Piperazineethanol, α-[(1,3-benzodioxol-5-yloxy)methyl] -4-(2-methoxyphenyl) (M2) as an inhibitor of BACH1. M2 and its analogues inhibited AFB1-induced porcine and human cell death in vitro, while M2 administration significantly improved AFB1-induced symptoms of weight loss and liver injury in vivo. These findings demonstrate that BACH1 plays a central role in AFB1-induced oxidative damage by regulating antioxidant gene expression. We also present a potent candidate small-molecule inhibitor in developing novel treatments for AFB1 toxicity.

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Aflatoxin B1 alters meat quality associated with oxidative stress, inflammation, and gut-microbiota in sheep

Cited by 37 publications

References 40 publications

Melatonin mitigates aflatoxin B1‐induced liver injury via modulation of gut microbiota/intestinal FXR/liver TLR4 signaling axis in mice

Melatonin mitigates aflatoxin B1‐induced liver injury via modulation of gut microbiota/intestinal FXR/liver TLR4 signaling axis in mice

Metabolomics approach reveals high energy diet improves the quality and enhances the flavor of black Tibetan sheep meat by altering the composition of rumen microbiota

Genome-Scale CRISPR Knockout Screening Identifies BACH1 as a Key Regulator of Aflatoxin B1-Induced Oxidative Damage

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