Affinity sensor for haemoglobin A1c based on single-walled carbon nanotube field-effect transistor and fructosyl amino acid binding protein

Hatada, Mika; Tran, Thien-Toan; Tsugawa, Wakako; Sode, Koji; Mulchandani, Ashok

doi:10.1016/j.bios.2018.09.069

Cited by 34 publications

(20 citation statements)

References 22 publications

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“…A broad spectrum of compounds that find applications in healthcare diagnosis and POC analysis of diseases can be detected with such biosensor materials [22]. Carbon allotrope-based nanomaterials consisting of fullerenes, [23,24,25,26,27] nanotubes (CNT) [28,29,30,31,32,33], films of graphene and its derivatives [34,35,36,37], quantum dots [38,39,40,41], and nanodiamonds [42,43,44,45,46,47] play a substantial role in recent advancements in the biosensor domain. In addition to greater sensitivity and novel mechanisms, such sensors offer a higher spatial resolution in case of localised detection along with real-time and label-free non-destructive sensing.…”

Section: Carbon Allotrope-based Nanomaterials Applications In Healtmentioning

confidence: 99%

“…They serve as scaffolds for biomolecule immobilisation, thereby ameliorating signal transduction and subsequently recognition [74]. The semiconducting nature of carbon nanotubes allows their use as nanoscale field effect transistors (FET) [32]. They can be used to manufacture top-of-the-line nanoscale electrodes due to the superior excellent conductivity along their length.…”

Section: Carbon Allotrope-based Nanomaterials Applications In Healtmentioning

confidence: 99%

“…Numerous CNT-based biosensors for glycaemic biomarkers of diabetes mellitus have been recently reported. Hatada et al reported a label-free chemiresistor-type FET affinity sensor for haemoglobin A1c (HbA1c) using single-walled carbon nanotubes (SWCNT) as a transducing element and a bacterial periplasmic protein (SocA) as a receptor [32]. HbA1c, on proteolytic hydrolysis, produces fructosyl valine (FV) that could be quantified by the sensor in a concentration range of 1.2–1909 nM.…”

Section: Carbon Allotrope-based Nanomaterials Applications In Healtmentioning

confidence: 99%

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Nanomaterials for Healthcare Biosensing Applications

Pirzada

Altıntaş

2019

Sensors

170

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In recent years, an increasing number of nanomaterials have been explored for their applications in biomedical diagnostics, making their applications in healthcare biosensing a rapidly evolving field. Nanomaterials introduce versatility to the sensing platforms and may even allow mobility between different detection mechanisms. The prospect of a combination of different nanomaterials allows an exploitation of their synergistic additive and novel properties for sensor development. This paper covers more than 290 research works since 2015, elaborating the diverse roles played by various nanomaterials in the biosensing field. Hence, we provide a comprehensive review of the healthcare sensing applications of nanomaterials, covering carbon allotrope-based, inorganic, and organic nanomaterials. These sensing systems are able to detect a wide variety of clinically relevant molecules, like nucleic acids, viruses, bacteria, cancer antigens, pharmaceuticals and narcotic drugs, toxins, contaminants, as well as entire cells in various sensing media, ranging from buffers to more complex environments such as urine, blood or sputum. Thus, the latest advancements reviewed in this paper hold tremendous potential for the application of nanomaterials in the early screening of diseases and point-of-care testing.

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Section: Carbon Allotrope-based Nanomaterials Applications In Healtmentioning

confidence: 99%

Section: Carbon Allotrope-based Nanomaterials Applications In Healtmentioning

confidence: 99%

Section: Carbon Allotrope-based Nanomaterials Applications In Healtmentioning

confidence: 99%

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Nanomaterials for Healthcare Biosensing Applications

Pirzada

Altıntaş

2019

Sensors

170

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“…Only few information about their isolation in good yields and purities has been reported, using either protected sugars as presented by the groups of Hoffmann and Horvat [18][19][20] or zinc halide catalysts as by the groups of Harohally and Norin [21][22][23]. The synthetic approach that mimics the natural reaction of free Glc with a primary amine of a protein occurring in the organism is currently still widely used by working groups studying Amadori compounds or developing HbA 1c assays [24][25][26][27][28]. However, this approach usually leads to a complex, dark syrup-like mixture containing various side products from which the desired fructosamine is subsequently crystallized.…”

Section: Introductionmentioning

confidence: 99%

Direct glucosone-based synthesis and HILIC-ESI-MS/MS characterization of N-terminal fructosylated valine and valylhistidine for validation of enzymatic HbA1c assays in the diagnosis of diabetes mellitus

Gerke

Buchholz

Müller³

et al. 2019

Anal Bioanal Chem

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Naturally occurring fructosamines are of high clinical significance due to their potential use in diabetes mellitus monitoring (quantification of fructosylated hemoglobin, HbA 1c ) or for the investigation of their reactivity in consecutive reactions and harmfulness towards the organism. Here we report the specific synthesis of the fructosylated dipeptide L-valyl-L-histidine (Fru-Val-His) and fructosylated L-valine (Fru-Val). Both are basic tools for the development and validation of enzymatic HbA 1c assays. The two fructosamine derivatives were synthesized via a protected glucosone intermediate which was coupled to the primary amine of Val or Val-His, performing a reductive amination reaction. Overall yields starting from fructose were 36% and 34% for Fru-Val and Fru-Val-His, respectively. Both compounds were achieved in purities > 90%. A HILIC-ESI-MS/MS method was developed for routine analysis of the synthesized fructosamines, including starting materials and intermediates. The presented method provides a well-defined and efficient synthesis protocol with purification steps and characterization of the desired products. The functionality of the fructosylated dipeptide has been thoroughly tested in an enzymatic HbA 1c assay, showing its concentration-dependent oxidative degradation by fructosyl-peptide oxidases (FPOX).

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“…Recently, the enzyme-based electrochemical biosensors have revealed even more advantages, such as, high selectivity, inexpensive fabrication, good portability and low real-time detection compared to the non-enzyme electrodes [4]. Several amino acid biosensors have been reported based on the several approaches of the electrode-positing [5], the gel matrix [6], the silica gel [7], and the carbonic materials [8] and so on. However, the enzyme electrodes revealed that the stereospecific oxidative deamination of amino acids can be catalyzed by the immobilized DAAO/LAAO [9,10].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical investigation of amino acids Parkia seeds using the composite electrode based on copper/carbon nanotube/nanodiamond

Babadi

Hosseini

Shavandi

et al. 2019

Journal of Environmental Chemical Engineering

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An electrochemical biosensor comprising copper, nano-diamond (ND) and carbon nanotube (CNT) has been fabricated to detect the amino acids of Parkia speciosa (PS) seeds. Parkia speciosa (stink bean), a Southeast Asian legume, is composed of medicinal chemicals which exhibit biological activities. The electro-catalytic activity of three electrodes Cu/CNT/ND, Zn/CNT/ND and NiO/CNT/ND was studied using 5 mM potassium ferrocyanide in 0.1 MKCl. The Zn/CNT/ND electrode exhibited irreversible reaction free oxidation with reduction peaks at −1 V, whereas, a pair redox peaks was observed for Cu/CNT/ND electrode. The immobilization of L-amino acid oxidase on the Cu/CNT/ND electrode was carried out to catalyze the amino acids detection. It was observed that the anodic and cathodic peak currents increased linearly with both the square root of the scan rate (ν 1/2 ) and scan rate (ν) over the studied scan range of 0.01-0.1 V/s with high correlation coefficients and following both the adsorption and the diffusion-controlled mechanisms. The developed biosensor displayed a very good electrocatalytic activity toward the oxidation of the amino acid to release H 2 O 2 and NH 3 as a result of the reaction between the active sites and the Parkia speciosa component. This was also confirmed by a drop in the pH value from 6.8 to 6.5 and a change in the color of the solution from green to yellow (releasing H 2 S). The impedance results indicated an inductance behavior due to the co-formation of the hydrogen peroxide (H 2 O 2 ) and the water via the adsorption on the electrode surface.

show abstract

Affinity sensor for haemoglobin A1c based on single-walled carbon nanotube field-effect transistor and fructosyl amino acid binding protein

Cited by 34 publications

References 22 publications

Nanomaterials for Healthcare Biosensing Applications

Nanomaterials for Healthcare Biosensing Applications

Direct glucosone-based synthesis and HILIC-ESI-MS/MS characterization of N-terminal fructosylated valine and valylhistidine for validation of enzymatic HbA1c assays in the diagnosis of diabetes mellitus

Electrochemical investigation of amino acids Parkia seeds using the composite electrode based on copper/carbon nanotube/nanodiamond

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