Affinity purification of major chitinases produced by Streptomyces kurssanovii

Purification and Characterization of Chitinases from the Nematophagous Fungi Verticillium chlamydosporium and V. suchlasporium

“…Purified endochitinase CHI43 exhibited activity comparable to endochitinases produced by S. kurssanovii (Tikhonov et al, 1998).…”

“…Furthermore, pore size of MPC-Chitin did not exclude any protein present in culture filtrates since it allows the entry of high-molecular-weight molecules, i.e., blue dextran, into the matrix (V. E. Tikhonov, unpublished results). In this respect MPC-Chitin has already been successfully used for separation of endochitinases produced by Streptomyces kurssanovii (Tikhonov et al, 1998;Ilyina et al, 2000). Hydrophobicity played a major role in early interactions among CHI43 and MPC-Chitin.…”

“…Aqueous colloidal chitin containing 10 mg of dried chitin/ml was sterilized at 120°C for 30 min and kept at 6°C before use. Macroporous cross-linked chitin (MPCchitin) 2 was prepared according to Tikhonov et al (1998) using macroporous beaded chitin produced by BioChit (Moscow, Russia). Chitosan produced by the same provider from Kamchatka-peninsula-shelf crab shell chitin had a degree of deacetylation of 80% with a molecular weight of 390 kDa.…”

Exploration of Microorganisms Producing Bioactive Molecules of Industrial Interest by Solid State Fermentation

“…It is estimated that there are between 10 and 25 different chitinases. Tikhonov et al (1998) produced and purified chitinases from Streptomyces kurssanovii. Brzezinska et al (2012) studied the degradation of chitin substances with chitinase from Streptomyces rimosus, which was isolated from soil.…”