Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes

Winczura, Kinga; Domanski, Michal; LaCava, John

doi:10.1007/978-1-4939-9822-7_15

Cited by 3 publications

(1 citation statement)

References 50 publications

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“…In addition to maintaining the biological stability of exosomes, lipids are also involved in biological processes such as the formation and release of exosomes[ 50 ]. Exosomes are also rich in a variety of proteins, including cytoskeleton components, tetraspanins, heat-shock proteins, and other types of proteins[ 51 , 52 ]. Among them, ALIX and tetraspanin proteins, such as CD81, CD9, and CD63, are markers of exosomes[ 51 ].…”

Section: Biological Characteristics and Functions Of Exosomesmentioning

confidence: 99%

Exosomes derived from stem cells as an emerging therapeutic strategy for intervertebral disc degeneration

Hu¹,

Li²,

Chang³

et al. 2020

WJSC

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Intervertebral disc (IVD) degenerative diseases are a common problem in the world, and they cause substantial social and economic burdens for people. The current methods for treating IVD degenerative diseases mainly include surgery and conservative treatment, which cannot fundamentally restore the normal structure of the disc. With continuous research on the mechanism of degeneration and the development of regenerative medicine, rapid progress has been made in the field of regenerative medicine regarding the use of stem cell-derived exosomes, which are active biological substances used in intercellular communication, because they show a strong effect in promoting tissue regeneration. The study of exosomes in the field of IVD degeneration has just begun, and many surprising achievements have been made. This paper mainly reviews the biological characteristics of exosomes and highlights the current status of exosomes in the field of IVD degeneration, as well as future developments regarding exosomes.

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Section: Biological Characteristics and Functions Of Exosomesmentioning

confidence: 99%

Exosomes derived from stem cells as an emerging therapeutic strategy for intervertebral disc degeneration

Hu¹,

Li²,

Chang³

et al. 2020

WJSC

View full text Add to dashboard Cite

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Affinity Isolation of Endogenous Saccharomyces Cerevisiae Nuclear Pore Complexes

Nudelman

Fernández-Martı́nez

Rout

2022

Methods in Molecular Biology

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Mesenchymal Stem Cell–Derived Extracellular Vesicles Protect Rat Nucleus Pulposus Cells from Oxidative Stress

et al. 2023

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Background Oxidative stress (OS) is mainly associated with the pathogenesis of intervertebral disc (IVD) degeneration; it causes nucleus pulposus cells (NPCs) to undergo senescence and triggers autophagy and apoptosis. This study aims to evaluate the regeneration potential of extracellular vesicles (EVs) derived from human umbilical cord-mesenchymal stem cells (hUC-MSCs) in an in vitro rat NPC-induced OS model. Design NPCs were isolated from rat coccygeal discs, propagated, and characterized. OS was induced by hydrogen peroxide (H2O2), which is confirmed by 2,7-dichlorofluorescein diacetate (H2DCFDA) assay. EVs were isolated from hUC-MSCs and characterized by analyzing the vesicles using fluorescence microscope, scanning electron microscope (SEM), atomic force microscope (AFM), dynamic light scattering (DLS), and Western blot (WB). The in vitro effects of EVs on migration, uptake, and survival of NPCs were determined. Results SEM and AFM topographic images revealed the size distribution of EVs. The phenotypes of isolated EVs showed that the size of EVs was 403.3 ± 85.94 nm, and the zeta potential was −0.270 ± 4.02 mV. Protein expression analysis showed that EVs were positive for CD81 and annexin V. Treatment of NPCs with EVs reduced H2O2-induced OS as evidenced by a decrease in reactive oxygen species (ROS) levels. Co-culture of NPCs with DiI-labeled EVs showed the cellular internalization of EVs. In the scratch assay, EVs significantly increased NPC proliferation and migration toward the scratched area. Quantitative polymerase chain reaction analysis showed that EVs significantly reduced the expression of OS genes. Conclusion EVs protected NPCs from H2O2-induced OS by reducing intracellular ROS generation and improved NPC proliferation and migration.

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Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes

Cited by 3 publications

References 50 publications

Exosomes derived from stem cells as an emerging therapeutic strategy for intervertebral disc degeneration

Exosomes derived from stem cells as an emerging therapeutic strategy for intervertebral disc degeneration

Affinity Isolation of Endogenous Saccharomyces Cerevisiae Nuclear Pore Complexes

Mesenchymal Stem Cell–Derived Extracellular Vesicles Protect Rat Nucleus Pulposus Cells from Oxidative Stress

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