Affinity partitioning of glucose-6-phosphate dehydrogenase and hexokinase in aqueous two-phase systems with free triazine dye ligands

Purification of lipase produced by a new source of Bacillus in submerged fermentation using an aqueous two-phase system

“…As a result, partitioning of the enzyme into the salt-rich bottom phase decreased. The same results were obtained by Xu et al [31], for the partitioning of glucose-6-phosphate dehydrogenase and hexokinase in PEG/PES systems, and by Bassani et al [11] partitioning porcine pancreatic lipase in PEG/potassium phosphate. During this pH change, the enzyme recovery from the bottom phase decreased from 91.59 to 85.69 (data not shown); moreover there was also a decrease in PF in the bottom phase, with the highest value (141.65 Fig.…”

Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system

“…1B and 1C). Same results were obtained by Xu et al (2002) who studied the extraction of glucose-6-phosphate dehydrogenase and hexokinase in PEG/PES systems. Wu et al (2001), working with two consecutive aqueous two phase extraction steps in PEG 8000/polyvynilalcohol system, followed in PEG 8000/(NH 4 ) 2 SO 4 system, observed strong partitioning of endopolygalacturonase into the bottom phase with the increase of pH.…”

Liquid–liquid extraction of an extracellular alkaline protease from fermentation broth using aqueous two-phase and reversed micelles systems

“…These results are corroborated by those obtained by Huddleston et al (1991), who studied the coefficient of partition of intracellular proteins from Saccharomyces cerevisiae in PEG/phosphate systems, observing for PEG 400 and 1000 preference of proteins for the top phase. Studies of Xu et al (2002) showed similar behaviour with hexokinase and glucose-6-phosphate dehydrogenase in PEG/PES systems. The importance of the protein molecular weight must also be considered, once the effect of polymer molecular mass is closely connected with the biomaterial molecular mass.…”