Affinity of N-acetylneuraminic acid for influenza virus neuraminidase

Walop, J. N.; Boschman, Th.A.C.; Jacobs, Judith M.

doi:10.1016/0006-3002(60)91544-4

Cited by 28 publications

(5 citation statements)

References 6 publications

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“…Neuraminidase activities of the supernatant fluids were determined by depletion of substrate, a modification of the method used for determining the inhibition of influenza virus neuraminidase by N-acetylneuraminic acid (8). Sodium borohydride was added after incubation of the enzyme with substrate to destroy the liberated N-acetylneuraminic acid; the uncleaved N-acetylneuraminic acid was determined with the Ehrlich reagent (10).…”

Section: Methodsmentioning

confidence: 99%

Neuraminidase Activity inDiplococcus pneumoniae

1966

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A method for the quantitation of neuraminidase in the presence of N-acetylneuraminic acid aldolase is described. The neuraminidase content of Diplococcus pneumoniae was found to be dependent on the media employed for growth; the highest enzyme activity per milligram of bacterial protein was obtained with Todd-Hewitt broth. Neuraminidase production was stimulated in D. pneumoniae by the addition of N-acetylneuraminlactose, N-acetylneuraminic acid, or N-acetylmannosamine to the growth medium. Three rough strains of D. pneumoniae, which were nonpathogenic for mice, lacked neuraminidase activity. Seven of 12 smooth strains contained neuraminidase; enzyme activity was not detected in the remaining 5 smooth strains. There was no correlation between the presence of neuraminidase activity and the capsular type or between neuraminidase production and animal virulence.

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Section: Methodsmentioning

confidence: 99%

Neuraminidase Activity inDiplococcus pneumoniae

1966

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“…Since it has been shown that the rdease of sialic acid from sialylactose by neuraminidase can be inhibited by addition of free sialic acid to the reaction mixture (21), it was of interest to determine the effect of adding neutralized sialic acid to the lymphocyte-enzyme mixture. The results of one of three similar experiments is shown in Table V and demonstrate that the effectiveness of the neuraminidase preparation in altering the distribution of radioactivity in recipients could be inhibited by adding sialic acid to the in vitro reaction mixture.…”

Section: Condition Of 51cr-labeled Lymphocytes At the Time Of Transfumentioning

confidence: 99%

The Effect of Neuraminidase on the Fate of Transfused Lymphocytes

Woodruff

Gesner

1969

The Journal of Experimental Medicine

270

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Evidence has been obtained that incubation of rat lymphocytes with neuraminidase, prior to intravenous transfusion into allogeneic or syngeneic recipients, alters the distribution of the cells. Many enzyme-treated lymphocytes initially become trapped in the liver, and there is a decrease in the selective accumulation of these cells in the lymph nodes and spleen. Subsequently, many enzyme-altered cells emigrate from the liver, concentrate in lymph nodes, and recirculate to the lymph. The results suggest that sialic acid constituents of the lymphocyte surface play a critical role in ensuring the normal distribution of these cells in the body. The findings also imply that reactions involving surface sialic acid can markedly alter the fate of lymphocytes without "killing" the cells.

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“…In presence of inhibitors Control (2) (3) (4) possess neuraminidases belonging to different kinetic groups [9] . The neuraminidases of the parainfluenza viruses of types 2 and 3 are not affected by inhibitors (2)- (5), which is indicative of a somewhat abnormal specificity of the enzymes in question.…”

Section: Virus Strain Nana Released (Pmoles)mentioning

confidence: 99%