Affinity ionic liquids for the rapid liquid–liquid extraction purification of hexahistidine tagged proteins

Ren, Guangwei; Gong, Xingwen; Wang, Bilei; Chen, Yingyi; Huang, Jianying

doi:10.1016/j.seppur.2015.03.025

Cited by 26 publications

(10 citation statements)

References 27 publications

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“…The addition of triazacyclononane-IL-sorbent to water resulted in purity values of 90% 268 and 95%. 267 In both works, 267 , 268 it was claimed that the sorbents used are easily regenerated in situ by the addition of EDTA followed by the reimmobilization of metal ions. It should be noted that all of the previously described works highlight the pivotal role of the IL water content in the IL phase for protein extraction, a crucial feature when dealing with hydrophobic ILs.…”

Section: Proteinsmentioning

confidence: 99%

Ionic-Liquid-Mediated Extraction and Separation Processes for Bioactive Compounds: Past, Present, and Future Trends

et al. 2017

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Ionic liquids (ILs) have been proposed as promising media for the extraction and separation of bioactive compounds from the most diverse origins. This critical review offers a compilation on the main results achieved by the use of ionic-liquid-based processes in the extraction and separation/purification of a large range of bioactive compounds (including small organic extractable compounds from biomass, lipids, and other hydrophobic compounds, proteins, amino acids, nucleic acids, and pharmaceuticals). ILs have been studied as solvents, cosolvents, cosurfactants, electrolytes, and adjuvants, as well as used in the creation of IL-supported materials for separation purposes. The IL-based processes hitherto reported, such as IL-based solid–liquid extractions, IL-based liquid–liquid extractions, IL-modified materials, and IL-based crystallization approaches, are here reviewed and compared in terms of extraction and separation performance. The key accomplishments and future challenges to the field are discussed, with particular emphasis on the major lacunas found within the IL community dedicated to separation processes and by suggesting some steps to overcome the current limitations.

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Section: Proteinsmentioning

confidence: 99%

Ionic-Liquid-Mediated Extraction and Separation Processes for Bioactive Compounds: Past, Present, and Future Trends

et al. 2017

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“…Alternative protein purification technologies such as crystallisation [10][11][12][13][14][15], precipitation [16], filtration [17][18][19] and extraction [20][21][22][23][24][25] have been developed to overcome these drawbacks of chromatography. Among these technologies, protein crystallisation is the most technically challenging option due to the difficulty of forming highly ordered structure with large flexible protein molecules [26,27].…”

Section: Introductionmentioning

confidence: 99%

Protein purification with nanoparticle-enhanced crystallisation

Chen

Cheng

Khaw

et al. 2021

Separation and Purification Technology

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In this study, silica nanoparticle was synthesised and used to promote lysozyme crystallisation effectively against high concentrations of protein impurity (bovine serum albumin (BSA); concentration = 25.0 -50.0 mg/mL vs 5.0 -25.0 mg/mL for lysozyme) at 1 mL scale, demonstrating that crystallisation is a viable and scalable protein purification technology with the aid of heterogeneous nucleants. The silica nanoparticle expedited the crystallisation of lysozyme through the enhancement of nucleation, significantly improving the process productivity. Furthermore, this study demonstrates the proper use of nanoparticle in terms of cycle time, as the improvement of productivity by silica nanoparticle has a monomodal peak shape over time.

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“…Given that, they have been used as functional molecules fixed with stationary phase or as additives in HPLC and CE for the separation of large bio-molecules [103][104][105]. In addition, TSILs served as the hydrophobic solvent in LLE and as the hydrophilic solvent in ATPS for the extraction of proteins were reported in the last ten years [106][107][108][109][110][111][112][113][114][115].…”

Section: Tsils For the Purification Of Proteins And Peptidesmentioning

confidence: 99%

“…A hydrophobic functional IL (Table , Entry 15) was introduced for the purification of target proteins . The common IL 1‐butyl‐3‐methylimidazolium bis(trifluoromethylsulfonyl)imide was employed as extraction agent.…”

Section: Tsils For the Purification Of Proteins And Peptidesmentioning

confidence: 99%

Recent progress of task‐specific ionic liquids in chiral resolution and extraction of biological samples and metal ions

Cai

Zhao

et al. 2017

J of Separation Science

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Ionic liquids have been functionalized for modern applications. The functional ionic liquids are also called task-specific ionic liquids. Various task-specific ionic liquids with certain groups have been constructed and exploited widely in the field of separation. To take advantage of their properties in separation science, task-specific ionic liquids are generally used in techniques such as liquid-liquid extraction, solid-phase extraction, gas chromatography, high-performance liquid chromatography, and capillary electrophoresis. This review mainly covers original research papers published in the last five years, and we will focus on task-specific ionic liquids as the chiral selectors in chiral resolution and as extractant or sensor for biological samples and metal ion purification. K E Y W O R D Schiral ionic liquids, chiral resolution, metal extraction, protein purification, task-specific ionic liquids

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Affinity ionic liquids for the rapid liquid–liquid extraction purification of hexahistidine tagged proteins

Cited by 26 publications

References 27 publications

Ionic-Liquid-Mediated Extraction and Separation Processes for Bioactive Compounds: Past, Present, and Future Trends

Ionic-Liquid-Mediated Extraction and Separation Processes for Bioactive Compounds: Past, Present, and Future Trends

Protein purification with nanoparticle-enhanced crystallisation

Recent progress of task‐specific ionic liquids in chiral resolution and extraction of biological samples and metal ions

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