Affinity chromatography revealed insights into unique functionality of two 14-3-3 protein species in developing maize kernels

Dou, Yao; Liu, Xiangguo; Yin, Yuejia; Han, Si‐ping; Yang, Liu; Liu, Yang; Hao, Dongyun

doi:10.1016/j.jprot.2014.10.019

Cited by 21 publications

(13 citation statements)

References 48 publications

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“…Several studies have reported that 14-3-3 proteins are involved in various cellular physiological processes, such as cell signal transduction, cell cycle regulation, nitrogen and carbon assimilation, and defense mechanisms [76, 77]. In barley and maize grain, 14-3-3 proteins might interact with starch synthesis related enzymes, such as ADPase, GBSS, SBE, and ISA [78, 79], indicating that they might be involved in the regulation of starch synthesis. Interestingly, in Arabidopsis leaves, reduction or over-expression of 14-3-3 proteins is correlated with dramatic increases or decreases, respectively, in starch content [80, 81].…”

Section: Resultsmentioning

confidence: 99%

Proteomic analysis of maize grain development using iTRAQ reveals temporal programs of diverse metabolic processes

Dong

et al. 2016

BMC Plant Biol

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BackgroundGrain development in maize is an essential process in the plant’s life cycle and is vital for use of the plant as a crop for animals and humans. However, little is known regarding the protein regulatory networks that control grain development. Here, isobaric tag for relative and absolute quantification (iTRAQ) technology was used to analyze temporal changes in protein expression during maize grain development.ResultsMaize grain proteins and changes in protein expression at eight developmental stages from 3 to 50 d after pollination (DAP) were performed using iTRAQ-based proteomics. Overall, 4751 proteins were identified; 2639 of these were quantified and 1235 showed at least 1.5-fold changes in expression levels at different developmental stages and were identified as differentially expressed proteins (DEPs). The DEPs were involved in different cellular and metabolic processes with a preferential distribution to protein synthesis/destination and metabolism categories. A K-means clustering analysis revealed coordinated protein expression associated with different functional categories/subcategories at different development stages.ConclusionsOur results revealed developing maize grain display different proteomic characteristics at distinct stages, such as numerous DEPs for cell growth/division were highly expressed during early stages, whereas those for starch biosynthesis and defense/stress accumulated in middle and late stages, respectively. We also observed coordinated expression of multiple proteins of the antioxidant system, which are essential for the maintenance of reactive oxygen species (ROS) homeostasis during grain development. Particularly, some DEPs, such as zinc metallothionein class II, pyruvate orthophosphate dikinase (PPDK) and 14-3-3 proteins, undergo major changes in expression at specific developmental stages, suggesting their roles in maize grain development. These results provide a valuable resource for analyzing protein function on a global scale and also provide new insights into the potential protein regulatory networks that control grain yield and quality.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0878-1) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Proteomic analysis of maize grain development using iTRAQ reveals temporal programs of diverse metabolic processes

Dong

et al. 2016

BMC Plant Biol

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“…The 14‐3‐3 protein family is highly expressed during grain (or seed) development (Hajduch et al ., ; Houston et al ., ). Large‐scale interactome studies conducted in maize kernels (Dou et al ., ), Arabidopsis seeds (Swatek et al ., ), and barley grains (Alexander and Morris, ) using affinity chromatography methods have revealed that 14‐3‐3 proteins interact with hundreds of potential client proteins that are involved in various metabolic pathways, therefore indicating the major role of 14‐3‐3 proteins in the development and filling of sink organs. The present study discovered markedly higher differences in transcript levels of GF14f, a member of the 14‐3‐3 protein family, during grain filling in SS and IS.…”

Section: Discussionmentioning

confidence: 99%

The 14‐3‐3 protein GF14f negatively affects grain filling of inferior spikelets of rice (Oryza sativa L.)

et al. 2019

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In rice (Oryza sativa L.), later flowering inferior spikelets (IS), which are located on proximal secondary branches, fill slowly and produce smaller and lighter grains than earlier flowering superior spikelets (SS). Many genes have been reported to be involved in poor grain filling of IS, however the underlying molecular mechanisms remain unclear. The present study determined that GF14f, a member of the 14-3-3 protein family, showed temporal and spatial differences in expression patterns between SS and IS. Using GF14f-RNAi plants, we observed that a reduction in GF14f expression in the endosperm resulted in a significant increase in both grain length and weight, which in turn improved grain yield. Furthermore, pull-down assays indicated that GF14f interacts with enzymes that are involved in sucrose breakdown, starch synthesis, tricarboxylic acid (TCA) cycle and glycolysis. At the same time, an increase in the activity of sucrose synthase (SuSase), adenosine diphosphate-glucose pyrophosphorylase (AGPase), and starch synthase (StSase) was observed in the GF14f-RNAi grains. Comprehensive analysis of the proteome and metabolite profiling revealed that the abundance of proteins related to the TCA cycle, and glycolysis increased in the GF14f-RNAi grains together with several carbohydrate intermediates. These results suggested that GF14f negatively affected grain development and filling, and the observed higher abundance of the GF14f protein in IS compared with SS may be responsible for poor IS grain filling. The study provides insights into the molecular mechanisms underlying poor grain filling of IS and suggests that GF14f could serve as a potential tool for improving rice grain filling.

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“…In Arabidopsis , expression of GF14 chi was exclusively detected in mature siliques and immature seeds (Daugherty et al, 1996 ). In maize, expression of 2 14-3-3 proteins changed significantly during seed development (Dou et al, 2014 ). Based on the evidence above, 14-3-3 genes showed potential involvement in regulating banana fruit development and postharvest ripening processes.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification, Phylogeny, and Expression Analyses of the 14-3-3 Family Reveal Their Involvement in the Development, Ripening, and Abiotic Stress Response in Banana

Ren

et al. 2016

Front. Plant Sci.

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Plant 14-3-3 proteins act as critical components of various cellular signaling processes and play an important role in regulating multiple physiological processes. However, less information is known about the 14-3-3 gene family in banana. In this study, 25 14-3-3 genes were identified from the banana genome. Based on the evolutionary analysis, banana 14-3-3 proteins were clustered into ε and non-ε groups. Conserved motif analysis showed that all identified banana 14-3-3 genes had the typical 14-3-3 motif. The gene structure of banana 14-3-3 genes showed distinct class-specific divergence between the ε group and the non-ε group. Most banana 14-3-3 genes showed strong transcript accumulation changes during fruit development and postharvest ripening in two banana varieties, indicating that they might be involved in regulating fruit development and ripening. Moreover, some 14-3-3 genes also showed great changes after osmotic, cold, and salt treatments in two banana varieties, suggested their potential role in regulating banana response to abiotic stress. Taken together, this systemic analysis reveals the involvement of banana 14-3-3 genes in fruit development, postharvest ripening, and response to abiotic stress and provides useful information for understanding the functions of 14-3-3 genes in banana.

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Affinity chromatography revealed insights into unique functionality of two 14-3-3 protein species in developing maize kernels

Cited by 21 publications

References 48 publications

Proteomic analysis of maize grain development using iTRAQ reveals temporal programs of diverse metabolic processes

Proteomic analysis of maize grain development using iTRAQ reveals temporal programs of diverse metabolic processes

The 14‐3‐3 protein GF14f negatively affects grain filling of inferior spikelets of rice (Oryza sativa L.)

Genome-Wide Identification, Phylogeny, and Expression Analyses of the 14-3-3 Family Reveal Their Involvement in the Development, Ripening, and Abiotic Stress Response in Banana

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