Affinity chromatography of antibodies directed against soybean lipoxygenase-1 and -2 and an enzyme-linked immunosorbent assay (elisa) for antibodies and lipoxygenases

Vernooy-Gerritsen, Marjan; Bos, Adri L.M.; Veldink, Gerrit A.; Vliegenthart, Johannes F.G.

doi:10.1016/0167-4838(83)90038-9

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1985

1993

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Cited by 6 publications

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“…Immunoglobulin G (Ig G) fractions of antisera were isolated by the precipitation of the sera with 0.15 M caprylic acid according to the method of Steinbuch and Audran (15). Anti-lipoxygenase-1 and -2 were affinity chromatographed over lipoxygenase-1 and -2 coupled to CNBr-activated sepharose 4B, respectively (16). Affinity-purified antibodies were analyzed by Ouchterlony double-diffusion tests (17).…”

Section: Methodsmentioning

confidence: 99%

Subcellular localization of lipoxygenase‐1 and‐2 in germinating soybean seeds and seedlings

Song

Love

Murphy

1990

J Americ Oil Chem Soc

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The subcellular localization of lipoxygenase-1 and -2 in the cotyledons of germinating soybean seeds [Glycine max (L.) Merr. AmSoy] and seedlings was investigated by direct labeling with immunogold complexes. Both lipoxygenase-1 and -2 were localized in the cytoplasm of all cotyledon cells. No gold labeling was observed on mitochondria, glyoxysomes, protein bodies, lipid bodies, or other organelles.

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Section: Methodsmentioning

confidence: 99%