Affinity adsorption and separation behaviors of avidin on biofunctional magnetic nanoparticles binding to iminobiotin

Sun, Shuguo; Ma, Meihu; Qiu, Ning; Huang, Xi; Cai, Zhaoxia; Huang, Qun; Hu, Xin

doi:10.1016/j.colsurfb.2011.06.039

Cited by 23 publications

(13 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1,2 Magnetic nanoparticle (MNP) based on Fe 3 O 4 can be applied in drug delivery, bioseparation, biosensing, contrast agents for magnetic resonance imaging (MRI), and for magnetically induced cancer hyperthermia. [3][4][5][6][7] As a drug carrier, MNP is usually composed of a superparamagnetic Fe 3 O 4 core and a polymer coating layer, which provides functional groups facilitating drug binding, inhibiting aggregation, and increasing colloidal stability. [8][9][10] Other than polymer coating, silica has been known to be one of the most ideal coating layers for Fe 3 O 4 -MNP due to its reliable chemical stability, biocompatibility, and reactivity with various coupling agents, making them suitable for conjugation with drugs for in vivo applications.…”

Section: Introductionmentioning

confidence: 99%

Targeted delivery of tissue plasminogen activator by binding to silica-coated magnetic nanoparticle

Chen¹,

Yang²,

Ma³

et al. 2012

IJN

View full text Add to dashboard Cite

Background and methods: Silica-coated magnetic nanoparticle (SiO 2 -MNP) prepared by the sol-gel method was studied as a nanocarrier for targeted delivery of tissue plasminogen activator (tPA). The nanocarrier consists of a superparamagnetic iron oxide core and an SiO 2 shell and is characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, superconducting quantum interference device, and thermogravimetric analysis. An amine-terminated surface silanizing agent (3-aminopropyltrimethoxysilane) was used to functionalize the SiO 2 surface, which provides abundant -NH 2 functional groups for conjugating with tPA. Results: The optimum drug loading is reached when 0.5 mg/mL tPA is conjugated with 5 mg SiO 2 -MNP where 94% tPA is attached to the carrier with 86% retention of amidolytic activity and full retention of fibrinolytic activity. In vitro biocompatibility determined by lactate dehydrogenase release and cell proliferation indicated that SiO 2 -MNP does not elicit cytotoxicity. Hematological analysis of blood samples withdrawn from mice after venous administration indicates that tPA-conjugated SiO 2 -MNP (SiO 2 -MNP-tPA) did not alter blood component concentrations. After conjugating to SiO 2 -MNP, tPA showed enhanced storage stability in buffer and operation stability in whole blood up to 9.5 and 2.8-fold, respectively. Effective thrombolysis with SiO 2 -MNP-tPA under magnetic guidance is demonstrated in an ex vivo thrombolysis model where 34% and 40% reductions in blood clot lysis time were observed compared with runs without magnetic targeting and with free tPA, respectively, using the same drug dosage. Enhanced penetration of SiO 2 -MNP-tPA into blood clots under magnetic guidance was confirmed from microcomputed tomography analysis. Conclusion: Biocompatible SiO 2 -MNP developed in this study will be useful as a magnetic targeting drug carrier to improve clinical thrombolytic therapy.

show abstract

Section: Introductionmentioning

confidence: 99%

Targeted delivery of tissue plasminogen activator by binding to silica-coated magnetic nanoparticle

Chen¹,

Yang²,

Ma³

et al. 2012

IJN

View full text Add to dashboard Cite

show abstract

“…Preparation and Glutaraldehyde Activation of PEI-Coated MNPs. MNPs were prepared via a facile solvothermal synthesis method, in which polyethyleneimine (PEI) was used as the protective agent to prevent the particles from aggregation [30]. The typical synthetic procedure was as follows.…”

Section: Preparation Of Functionalized Mnpsmentioning

confidence: 99%

“…where [30][31][32][33], with slight modifications. Cytotoxicity of drugs was measured as previously described.…”

Section: Drug Loading Determination (Indirect Estimationmentioning

confidence: 99%

Preparation and Evaluation of Smart Nanocarrier Systems for Drug Delivery Using Magnetic Nanoparticle and Avidin-Iminobiotin System

Sun

Yang

et al. 2018

Journal of Nanomaterials

Self Cite

View full text Add to dashboard Cite

Therapeutic efficacy and the regulation of drug release can be improved by using selective targeting drug delivery systems. In this paper, we have demonstrated avidin-immobilized magnetic nanoparticles (AMNPs) as a novel targeted drug delivery system to deliver iminobiotinylated daunomycin (IDAU). TEM, XRD, VSM, and FTIR were employed for the physicochemical characterization of the drug-loaded MNPs. The binding of IDAU had little effect on sizes of AMNPs (~35 nm), but the stability and dispersibility of the nanoparticles were improved. The study also found that the loading capacity and efficiency of nanoparticles were mainly dependent on affinity interaction between IDAU and AMNPs. The optimal loading capacity and efficiency of MNPs for IDAU were 0.408 ± 0.012 mg/g and 94.18 ± 2.64% according to the reversed-phase high-performance liquid chromatography (RP-HPLC) data, respectively. Under the conditions of pH 6.8 and 1 mmol/L of biotin, the drug-loaded MNPs released rapidly at beginning and then maintained at a certain controllable release level. The effect of IDAU on DLKP proliferation was tested, and the results showed that IC50 was (1.60 ± 0.05) × 10−3 mg/mL. Our findings indicated that AMNPs hold tremendous potential as an effective drug delivery system.

show abstract

“…The strong binding affinity of biotin towards avidin is stable during proteolysis and within a wide range of pH and temperature levels and with a variety of denaturing agents. 14,[21][22][23] The EDC/NHS linker directly connects carboxylic and amino groups, for conjugating molecules with multiple carboxylic and amino groups. The functionalization procedure of Ab MNPs is shown in the schematic diagram (Fig.…”

Section: Functionalization Of Antibody Magnetic Nanoparticles (Ab Mnps)mentioning

confidence: 99%

Lymphoma cell isolation using multifunctional magnetic nanoparticles: antibody conjugation and characterization

Sahoo

Liu

2017

RSC Adv.

View full text Add to dashboard Cite

show abstract

Affinity adsorption and separation behaviors of avidin on biofunctional magnetic nanoparticles binding to iminobiotin

Cited by 23 publications

References 36 publications

Targeted delivery of tissue plasminogen activator by binding to silica-coated magnetic nanoparticle

Targeted delivery of tissue plasminogen activator by binding to silica-coated magnetic nanoparticle

Preparation and Evaluation of Smart Nanocarrier Systems for Drug Delivery Using Magnetic Nanoparticle and Avidin-Iminobiotin System

Lymphoma cell isolation using multifunctional magnetic nanoparticles: antibody conjugation and characterization

Contact Info

Product

Resources

About