Affective and cognitive behavior is not altered by chronic constriction injury in B7-H1 deficient and wildtype mice

Abstract

Background Chronic neuropathic pain is often associated with anxiety, depressive symptoms, and cognitive impairment with relevant impact on patients` health related quality of life. To investigate the influence of a pro-inflammatory phenotype on affective and cognitive behavior under neuropathic pain conditions, we assessed mice deficient of the B7 homolog 1 (B7-H1), a major inhibitor of inflammatory response. Results Adult B7-H1 ko mice and wildtype littermates (WT) received a chronic constriction injury (CCI) of the sciatic nerve, and we assessed mechanical and thermal sensitivity at selected time points. Both genotypes developed mechanical ( p < 0.001) and heat hypersensitivity ( p < 0.01) 7, 14, and 20 days after surgery. We performed three tests for anxiety-like behavior: the light–dark box, the elevated plus maze, and the open field. As supported by the results of these tests for anxiety-like behavior, no relevant differences were found between genotypes after CCI. Depression-like behavior was assessed using the forced swim test. Also, CCI had no effect on depression like behavior. For cognitive behavior, we applied the Morris water maze for spatial learning and memory and the novel object recognition test for object recognition, long-, and short-term memory. Learning and memory did not differ in B7-H1 ko and WT mice after CCI. Conclusions Our study reveals that the impact of B7-H1 on affective-, depression-like- and learning-behavior, and memory performance might play a subordinate role in mice after nerve lesion.

Transcriptome Analysis of the Mouse Medial Prefrontal Cortex in a Chronic Constriction Injury Model

“…However, Tyrtyshnaia's study found that short-term memory in CCI mice was signi cantly impaired as early as 14 days after surgery (Tyrtyshnaia et al 2017). Other study indicated that mice after CCI did not develop cognitive impairment examined by the novel object recognition test (Karl et al 2019). These discrepancies could be attributed to different behavioral protocols (the training protocol of Y-Maze used in Tyrtyshnaia's study is different from ours) or experiment conditions, for example different types of suture make the difference of the tension of the ligatures (Maves et al 1993), as there is a great inter-and intra-individual experimenter variability.…”

Morris water maze: a versatile and pertinent tool for assessing spatial learning and memory

“…5B ). Additionally, this parameter is also used in other MWM studies [ 95 , 156 , 167 , 168 ], highlighting its relevance for probe trial analysis.…”

Role of the PD‐1/PD‐L1 Signaling in Multiple Sclerosis and Experimental Autoimmune Encephalomyelitis: Recent Insights and Future Directions

“…Due to the involvement of B cells, NK cells, DCs, microglia/macrophages in MS pathology, the expression of PD-1/PD-L1 on these cells may also engage in MS development. Specifically, PD-1/PD-L1 not only limits autoimmunity in the CNS, but also exerts influences on acute and chronic pain, affective and cognitive behaviors [199,200]. Therefore, additional physiological functions of PD-1/PD-L1 in the CNS may provide new insights in MS pathogenesis.…”