AF4-UV/VIS-MALS-ICPMS/MS for the characterization of the different nanoparticulated species present in oligonucleotide-gold nanoparticle conjugates

An in-depth physicochemical investigation of drug-loaded core–shell UiO66 nanoMOFs

“…27 Moreover, as we have recently demonstrated, combination of AF4 with online molecular (MALS) and elemental (ICP-MS/MS) detectors is an invaluable tool to characterize nanostructures since it can separate them (AF4), determine their hydrodynamic size (MALS), quantify the elemental composition and determine stoichiometries and molar ratios (ICP-MS/MS) all together in one single analysis. 29…”

“…Sample recovery calculation and signal integration was done as described elsewhere. 29 ICP-MS operational configuration was similar to the previously described for total metal content determination (see ESI†).…”

“…27 Moreover, as we have recently demonstrated, combination of AF4 with online molecular (MALS) and elemental (ICP-MS/MS) detectors is an invaluable tool to characterize nanostructures since it can separate them (AF4), determine their hydrodynamic size (MALS), quantify the elemental composition and determine stoichiometries and molar ratios (ICP-MS/MS) all together in one single analysis. 29 The optimum AF4-MALS-ICP-MS/MS instrumental conditions are shown in Tables S1 and S2. † CD-MO@UiO66 nano-MOFs and pure cisplatin were separately injected and analyzed, and the fractograms are shown in Fig.…”