Aetiology of Acute Undifferentiated Fever Among Children Under the Age of Five in Vietnam: A Prospective Study

Tran, Xuan Duong; Hoang, Van Thuan; Dang, Tai Tan; Vu, Thi Phuong; To, Minh Manh; Tran, Trong Kiem; Dung, Manh; Nguyen, Duy Cuong; Nguyen, Quoc Tien; Colson, Philippe; Parola, Philippe; Marty, Pierre; Nguyen, Nhu Ngoc

doi:10.1007/s44197-023-00121-4

Cited by 4 publications

(1 citation statement)

References 32 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bottles were routinely sub-cultured onto solid media after 24 h and seven days, with additional subculture if turbidity was noted upon daily inspection. Bacterial isolates from blood were identified using the VITEK Mass Spectrometry System (Vitek MS; bioMérieux, Durham, NC, USA) 9 .…”

Section: Methodsmentioning

confidence: 99%

Viral and bacterial microorganisms in Vietnamese children with severe and non-severe pneumonia

Tran,

Hoang,

Goumballa

et al. 2024

Sci Rep

Self Cite

View full text Add to dashboard Cite

To investigate potential respiratory pathogens in children with community-acquired pneumonia (CAP) and risk factors for severe disease. This prospective study was conducted among 467 children at the Thai Binh Paediatric Hospital, Vietnam between 1 July 2020 and 30 June 2021. Clinical data and laboratory results were collected. Twenty-four respiratory microorganisms were tested from nasopharyngeal swabs using real-time PCR. Logistical regression was used to estimate a factor’s adjusted odd ratios of the severity of disease. Mean age of patients = 15.4 ± 13.3 months, 63.0% were male. Over 97% of patients had a positive PCR result. 87% of patients were positive for multiple (up to eight) microorganisms. Rhinovirus (46%), respiratory syncytial virus (RSV) (24%), enterovirus (17%), and parainfluenza viruses-3 (13%) were the most frequent viruses. H. influenzae (61%), S. pneumoniae (45%) and M. catarrhalis (30%) were the most common bacteria. 128 (27%) cases were classified as severe pneumonia. Presence of smokers at home (aOR 2.11, 95% CI 1.27–3.52, P value = 0.004), CRP level ≥ 50 mg/dL (aOR 6.11, 95% CI 3.86–9.68, P value < 0.0001), RSV (aOR 1.78, 95% CI 1.07–2.96, P value = 0.03) and H. influenzae (aOR 1.66, 95% CI 1.03–2.67, P value = 0.04) PCR detection associated with a higher risk of severe pneumonia; ,. Causative agents of pneumonia in children are complex. Children positive with RSV and H. influenzae need to be closely monitored to prevent severe pneumonia.

show abstract

Section: Methodsmentioning

confidence: 99%

Viral and bacterial microorganisms in Vietnamese children with severe and non-severe pneumonia

Tran,

Hoang,

Goumballa

et al. 2024

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

Aetiology of community-acquired pneumonia among infants and children at a hospital in Vietnam: screening for multiple pathogens using molecular methods

Tran

Hoang

Goumballa

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Objective: To investigate potential respiratory pathogens in children with community-acquired pneumonia (CAP) and risk factors for severe disease in a Vietnamese hospital. Methods: Clinical data and laboratory results were collected. Twenty-four respiratory pathogens were tested from nasopharyngeal swabs using real-time PCR. Results: 467 children with CAP were included, mean age = 15.4±13.3 months, 63.0% were male. Over 97% of patients had a positive PCR result. Majority of patients (87%) were positive for multiple (up to eight) pathogens. Rhinovirus (46%), respiratory syncytial virus (RSV) (24%), enterovirus (17%), and parainfluenza viruses-3 (13%) were the most frequent viruses. H. influenzae (61%), S. pneumoniae (45%) and M. catarrhalis (30%) were the most common bacteria. 128 (27 %) cases were classified as severe pneumonia. Presence of smokers at home, CRP level ≥ 50 mg/dL, RSV and H. influenzae PCR detection associated with a higher risk of severe pneumonia (aOR=2.11, P value=0.004; aOR=6.11, P value<0.0001; aOR=1.78, P value=0.03 and aOR=1.66, P value=0.04, respectively). Conclusion: Causative agents of pneumonia in children are complex. Case-controlled and metagenomic studies need to be performed to further investigate the role of the presence of microbial agents in the respiratory tract on pneumonia in children to differentiate between colonisation and infection.

show abstract

The Association of HHV-6 and the TNF-α (-308G/A) Promotor with Major Depressive Disorder Patients and Healthy Controls in Thailand

Sumala,

Ekalaksananan,

Pientong

et al. 2023

Viruses

View full text Add to dashboard Cite

Major depressive disorder (MDD) is a silent global health problem that can lead to suicide. MDD development is suggested to result from numerous risk factors, including genetic factors. A precise tool for MDD diagnosis is currently not available. Recently, inflammatory processes have been identified as being strongly involved in MDD development and the reactivation of human herpesvirus type 6 (HHV-6), upregulating cytokines such as TNF-α, which are associated with MDD. Therefore, this study aimed to determine the association of HHV-6 with genetic factors, especially TNF-α mutation, in MDD patients and their relatives compared to healthy controls. The Patient Health Questionnaire (PHQ-9) was used to evaluate MDD status, and 471 oral buccal samples were investigated for HHV-6 infection and viral copy number by qPCR. TNF-α (-308G/A) gene mutation and the cytokines TNF-α, IL-6, and IL-10 were analyzed by high-resolution melting (HRM) analysis and enzyme-linked immunosorbent assay (ELISA). Whole-exome sequencing of buccal samples was performed to analyze for genetic factors. The results showed significantly higher HHV-6 positivities and viral loads in MDD patients (15/59 (25.67%) and 14,473 ± 16,948 copies/µL DNA) and their relatives (blood relatives 17/36 (47.22%) and 8146 ± 5656 copies/µL DNA); non-blood relatives 7/16 (43.75%) and 20,721 ± 12,458 copies/µL DNA) compared to the healthy population (51/360 (14.17%) and 6303 ± 5791 copies/µL DNA). The TNF-α (-308G/A) mutation showed no significant difference. Surprisingly, 12/26 (46.15%) participants with the TNF-α (-308G/A) mutation showed HHV-6 positivities at higher rates than those with wild-type TNF-α (-308G) (70/267 (26.22%)). HHV-6-positive participants with TNF-α (-308G/A) showed higher levels of TNF-α, IL-6, and IL-10 than those of negative control. Exome analysis revealed that common mutations in immune genes were associated with depression. Therefore, this study unveiled the novel association of inflammatory gene TNF-α (-308G/A) mutations with HHV-6 reactivation, which could represent a combined risk factor for MDD. This result could induce further research on MDD development and clinical applications.

show abstract

Aetiology of Acute Undifferentiated Fever Among Children Under the Age of Five in Vietnam: A Prospective Study

Cited by 4 publications

References 32 publications

Viral and bacterial microorganisms in Vietnamese children with severe and non-severe pneumonia

Viral and bacterial microorganisms in Vietnamese children with severe and non-severe pneumonia

Aetiology of community-acquired pneumonia among infants and children at a hospital in Vietnam: screening for multiple pathogens using molecular methods

The Association of HHV-6 and the TNF-α (-308G/A) Promotor with Major Depressive Disorder Patients and Healthy Controls in Thailand

Contact Info

Product

Resources

About