Aerosol from a candidate modified risk tobacco product has reduced effects on chemotaxis and transendothelial migration compared to combustion of conventional cigarettes

Abstract

Reduction of harmful constituents by heating rather than combusting tobacco is a promising new approach to reduce harmful effects associated with cigarette smoking. We investigated the effect from a new candidate modified risk tobacco product, the tobacco heating system (THS) 2.2, on the migratory behavior of monocytes in comparison with combustible 3R4F reference cigarettes. The monocytic cell line (THP-1) and human coronary arterial endothelial cells (HCAECs) were used to analyze chemotaxis and transendothelial migration (TEM). To assess the influence of aerosol extract from THS2.2 and smoke extract from 3R4F on toxicity and inflammation, flow cytometry and ELISA assays were performed. The results show that treatment of THP-1 cells with extract from 3R4F or THS2.2 induced concentration-dependent increases in cytotoxicity and inflammation. The inhibitory effects of THS2.2 extract for chemotaxis and TEM were ∼18 times less effective compared to 3R4F extract. Furthermore, extract from 3R4F or THS2.2 induced concentration-dependent decreases in the integrity of HCAEC monolayer. For all examined endpoints, the extract from 3R4F showed more than one order of magnitude stronger effects than that from THS2.2 extract. These data indicate the potential of a heat not burn tobacco product to reduce the risk for cardiovascular disease compared to combustible cigarettes.

Effects of Cigarette Smoke, Cessation, and Switching to Two Heat-Not-Burn Tobacco Products on Lung Lipid Metabolism inC57BL/6andApoe−/−Mice—An Integrative Systems Toxicology Analysis

“…For example, we have already leveraged the Apoe −/− mouse study to concomitantly evaluate the reduction potential of THS2.2 aerosol for cardiovascular endpoints ( Phillips et al , 2015b ). In another example, to evaluate a relevant mechanism of the CS-mediated cardiovascular effects (ie, atherosclerosis), we have compared the effects of THS2.2 and 3R4F on chemotaxis and transendothelial migration of a monocytic cell line ( van der Toorn et al. , 2015 ).…”

New ideas, old problems? Heated tobacco products – a systematic review

“…The aqueous aerosol extract from IQOS has reduced effects on the adhesion of monocytic cells to human coronary endothelial cells compared to the aqueous reference cigarette smoke extract [55]. The aerosol extracts from IQOS were also found to induce less inflammation and migration, and to be less cytotoxic than those from burning conventional cigarettes [56]. The IQOS aerosol exerted a weaker biological impact on human organotypic bronchial epithelial cells than cigarette smoke at similar nicotine concentrations [57].…”

Oral Trauma and Tooth Avulsion Following Explosion of E-Cigarette

“…4 There have been some reported benefits from using E-cigarettes compared with conventional cigarettes, with potentially lower risk factors for cardiovascular disease in E-cigarette vapors versus tobacco smoke. 5 These effects are controversial and are countered by reports citing ill effects of the soluble components of E-cigarette vapor on endothelial barrier function promoting inflammation. 6 Animal studies have suggested that aerosol ingestion of E-cigarettes is safer than that of traditional cigarette smoke.…”