Abstract:We have assembled an aerosol-fluorescence spectrum analyzer (AFS), which can measure the fluorescence spectra and elastic scattering of airborne particles as they flow through a laser beam. The aerosols traverse a scattering cell where they are illuminated with intense (50 kW/cm(2)) light inside the cavity of an argon-ion laser operating at 488 nm. This AFS can obtain fluorescence spectra of individual dye-doped polystyrene microspheres as small as 0.5 µm in diameter. The spectra obtained from microspheres dop… Show more
“…However, these technical and scientific developments have been significant contributions to the scientific community (e.g. Hill et al, 1995;Cheng et al, 1999;Reyes et al, 1999;Seaver et al, 1999;Kopczynski et al, 2005;Cabredo et al, 2007;Campbell et al, 2007;Manninen et al, 2008;Pan et al, 2009;Sivaprakasam et al, 2009).…”
A B S T R A C T Atmospheric aerosol particles of biological origin are a very diverse group of biological materials and structures, including microorganisms, dispersal units, fragments and excretions of biological organisms. In recent years, the impact of biological aerosol particles on atmospheric processes has been studied with increasing intensity, and a wealth of new information and insights has been gained. This review outlines the current knowledge on major categories of primary biological aerosol particles (PBAP): bacteria and archaea, fungal spores and fragments, pollen, viruses, algae and cyanobacteria, biological crusts and lichens and others like plant or animal fragments and detritus. We give an overview of sampling methods and physical, chemical and biological techniques for PBAP analysis (cultivation, microscopy, DNA/RNA analysis, chemical tracers, optical and mass spectrometry, etc.). Moreover, we address and summarise the current understanding and open questions concerning the influence of PBAP on the atmosphere and climate, i.e. their optical properties and their ability to act as ice nuclei (IN) or cloud condensation nuclei (CCN). We suggest that the following research activities should be pursued in future studies of atmospheric biological aerosol particles: (1) develop efficient and reliable analytical techniques for the identification and quantification of PBAP; (2) apply advanced and standardised techniques to determine the abundance and diversity of PBAP and their seasonal variation at regional and global scales (atmospheric biogeography); (3) determine the emission rates, optical properties, IN and CCN activity of PBAP in field measurements and laboratory experiments; (4) use field and laboratory data to constrain numerical models of atmospheric transport, transformation and climate effects of PBAP.
“…However, these technical and scientific developments have been significant contributions to the scientific community (e.g. Hill et al, 1995;Cheng et al, 1999;Reyes et al, 1999;Seaver et al, 1999;Kopczynski et al, 2005;Cabredo et al, 2007;Campbell et al, 2007;Manninen et al, 2008;Pan et al, 2009;Sivaprakasam et al, 2009).…”
A B S T R A C T Atmospheric aerosol particles of biological origin are a very diverse group of biological materials and structures, including microorganisms, dispersal units, fragments and excretions of biological organisms. In recent years, the impact of biological aerosol particles on atmospheric processes has been studied with increasing intensity, and a wealth of new information and insights has been gained. This review outlines the current knowledge on major categories of primary biological aerosol particles (PBAP): bacteria and archaea, fungal spores and fragments, pollen, viruses, algae and cyanobacteria, biological crusts and lichens and others like plant or animal fragments and detritus. We give an overview of sampling methods and physical, chemical and biological techniques for PBAP analysis (cultivation, microscopy, DNA/RNA analysis, chemical tracers, optical and mass spectrometry, etc.). Moreover, we address and summarise the current understanding and open questions concerning the influence of PBAP on the atmosphere and climate, i.e. their optical properties and their ability to act as ice nuclei (IN) or cloud condensation nuclei (CCN). We suggest that the following research activities should be pursued in future studies of atmospheric biological aerosol particles: (1) develop efficient and reliable analytical techniques for the identification and quantification of PBAP; (2) apply advanced and standardised techniques to determine the abundance and diversity of PBAP and their seasonal variation at regional and global scales (atmospheric biogeography); (3) determine the emission rates, optical properties, IN and CCN activity of PBAP in field measurements and laboratory experiments; (4) use field and laboratory data to constrain numerical models of atmospheric transport, transformation and climate effects of PBAP.
“…These can be divided into three groups. The first group includes trials and studies to design and test an instrument capable of differentiating between biological and non biological aerosols such as a Fluorescence Spectrum Analyser and an Ultraviolet Aerodynamic Particle Sizer (UVAPS) (Brosseau et al, 2000;Chen et al, 1996;Hariston et al, 1997;Hill et al, 1995;Ho et al, 1999;Kaye et al, 2000;Nachman et al, 1996;Pan et al, 2003;Pinnick et al, 1998;Pinnick et al, 1995). The second group of studies aimed at designing and testing an instrument with the capability to characterise particle composition in order to discriminate between the bioaerosols themselves (Cheng et al, 1999;Pan et al, 1999;Seaver et al, 1999;Sivaprakasam et al, 2004;Weichert et al, 2002).…”
This work focused on two main outcomes. The first was the assessment of the response of the Ultraviolet Aerodynamic Particle Sizer Spectrometer (UVAPS) for two different fungal spore species.The UVAPS response was investigated as a function of fungal age and the frequency of air current that their colonies exposure to. This outcome was achieved through the measurement of fungal spore fluorescent percentage and fluorescent intensity throughout a period of culturing time (three weeks), and the study of their fluorescent percentage as a function of exposure to air currents. The second objective was to investigate the change of fungal spore size during this period, which may be of use as a co-factor in this differentiation. Fungal spores were released by blowing the surface of the culture colonies with continuous filtered flow air. The UVAPS was used to detect and measure autofluorescing biomolecules such as riboflavin and nicotinamide adenine dinucleotide phosphate (NAD(P)H) present in the released fungal spores.The study demonstrated an increase in aerodynamic diameter for fungal spores under investigation (Aspergillus niger and Penicillium species) over a period of time. The fluorescent percentage of spores was found to decrease for both fungal genera as they aged. It was also found that the fluorescent percentage for tested fungi decreased with frequency of air exposure. The results showed that, while the UVAPS could discriminate between Aspergillus and Penicillium species under well-controlled laboratory conditions, it is unlikely to be able to do so in the field.
“…The emission between 1600 and 2700 cm À 1 is remarkably high compared with most spectra of biological cells, proteins, nucleic acids, lipids, etc., when they are excited at wavelengths that generate less fluorescence. Light at 488 nm is known to excite relatively strong fluorescence in bacteria and tree leaves [8]. Sporopollenin in pollens and fungal spores is reported to fluoresce in the 400-650 nm range with high fluorescence intensity when excited at 300-550 nm [56,57].…”
Section: Comparison Of Ptrs Spectra Of Three Pollens and One Type Of mentioning
confidence: 99%
“…Some of these instruments measure single particle elastic scattering, and/or single particle fluorescence, and/or laser-or spark-induced breakdown spectroscopy (LIBS/SIBS) and some are commercially available [3]. Laser induced fluorescence (LIF), especially dual-wavelength UV-LIF has been demonstrated for near-real time detection and partial classification of bioaerosols particles [4][5][6][7][8][9]. The technique was shown to be capable of differentiating pollens from various plant species [9,10].…”
a b s t r a c tPhotophoretic trapping-Raman spectroscopy (PTRS) is a new technique for measuring Raman spectra of particles that are held in air using photophoretic forces. It was initially demonstrated with Raman spectra of strongly-absorbing carbon nanoparticles (Pan et al.[44] (Opt Express 2012)). In the present paper we report the first demonstration of the use of PTRS to measure Raman spectra of absorbing and weakly-absorbing bioaerosol particles (pollens and spores). Raman spectra of three pollens and one smut spore in a size range of 6.2-41.8 mm illuminated at 488 nm are shown. Quality spectra were obtained in the Raman shift range of 1600-3400 cm À 1 in this exploratory study. Distinguishable Raman scattering signals with one or a few clear Raman peaks for all four aerosol particles were observed within the wavenumber region 2940-3030 cm À 1 . Peaks in this region are consistent with previous reports of Raman peaks in the 1600-3400 cm À 1 range for pollens and spores excited at 514 nm measured by a conventional Raman spectrometer. Noise in the spectra, the fluorescence background, and the weak Raman signals in most of the 1600-3400 cm À 1 region make some of the spectral features barely discernable or not discernable for these bioaerosols except the strong signal within 2940-3030 cm À 1 . Up to five bands are identified in the three pollens and only two bands appear in the fungal spore, but this may be because the fungal spore is so much smaller than any of the pollens. The fungal spore signal relative to the air-nitrogen Raman band is approximately 10 times smaller than that ratio for the pollens. The five bands are tentatively assigned to the CH 2 symmetric stretch at 2948 cm À 1 , CH 2 Fermi resonance stretch at 2970 cm À 1 , CH 3 symmetric stretch at 2990 cm À 1 , CH 3 out-of-plane end asymmetric stretch at 3010 cm À 1 , and unsaturated ¼ CH stretch at 3028 cm À 1 . The two dominant bands of the up-to-five Raman bands in the 2940-3030 cm À 1 region have a consistent band spacing of 25 cm À 1 in all four aerosols. Finally we discuss improvements to the PTRS that should provide a system which can trap a higher fraction of particle types and obtain Raman spectra over a larger range (e.g., 200-3600 cm À 1 ) than those achieved here.Published by Elsevier Ltd.
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