Aerosol characterization and lung deposition of synthesized TiO2 nanoparticles for murine inhalation studies

Koivisto, Antti Joonas; Mäkinen, Marko; Rossi, Elina M.; Lindberg, Hanna; Miettinen, Merja; Falck, Ghita C.-M.; Norppa, Hannu; Alenius, Harri; Korpi, Anne; Riikonen, Joakim; Vanhala, Esa; Vippola, Minnamari; Pasanen, Pertti; Lehto, Vesa‐Pekka; Savolainen, Kai; Jokiniemi, Jorma; Hämeri, Kaarle

doi:10.1007/s11051-010-0186-x

Cited by 9 publications

(8 citation statements)

References 44 publications

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“…The deposition mass of NPs is correlated with the exposure concentration. Koivisto et al [10] found that the lung deposition mass increased though inhalation from 0.2 to 84.0 μg as the aerosol mass concentration increased from 0.8 to 28.5 mg m –3 , showing a linear correlation with aerosol mass concentration. However, few NPs were deposited in the organs.…”

Section: Introductionmentioning

confidence: 99%

Low doses of TiO₂-polyethylene glycol nanoparticles stimulate proliferation of hepatocyte cells

Sun

Kanehira

Taniguchi

2016

Science and Technology of Advanced Materials

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This paper describes the effect of low concentrations of 100 nm polyethylene glycol-modified TiO2 nanoparticles (TiO2-PEG NPs) on HepG2 hepatocellular carcinoma cells. Proliferation of HepG2 cells increased significantly when the cells were exposed to low doses (<100 μg ml–1) of TiO2-PEG NPs. These results were further confirmed by cell counting experiments and cell cycle assays. Cellular uptake assays were performed to determine why HepG2 cells proliferate with low-dose exposure to TiO2-PEG NPs. The results showed that exposure to lower doses of NPs led to less cellular uptake, which in turn decreased cytotoxicity. We therefore hypothesized that TiO2-PEG NPs could affect the activity of hepatocyte growth factor receptors (HGFRs), which bind to hepatocyte growth factor and stimulate cell proliferation. The localization of HGFRs on the surface of the cell membrane was detected via immunofluorescence staining and confocal microscopy. The results showed that HGFRs aggregate after exposure to TiO2-PEG NPs. In conclusion, our results indicate that TiO2-PEG NPs have the potential to promote proliferation of HepG2 cells through HGFR aggregation and suggest that NPs not only exhibit cytotoxicity but also affect cellular responses.

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Section: Introductionmentioning

confidence: 99%

Low doses of TiO₂-polyethylene glycol nanoparticles stimulate proliferation of hepatocyte cells

Sun

Kanehira

Taniguchi

2016

Science and Technology of Advanced Materials

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“…Start-up or induction period was already reported, e.g., by Kirkbir and Komiyama (1987) or Komiyama et al (1984) and mentioned also by Koivisto et al (2011). This gradual increase of NP production was attributed to the catalytic effect of TiO 2 deposit on the reactor wall (surface reaction).…”

Section: Particle Productionmentioning

confidence: 64%

“…On the other hand, an increasing GMD from 19 to 37 nm with increasing T R from 400 C to 600 C was reported by Okuyama et al (1986), but material of the reactor was not specified. Formation of NPs, consisting of crystallites in a size range from 10 to 60 nm, in a stainless steel reactor at 500 C was reported by Koivisto et al (2011). Very small NPs, with mean sizes ranging between 6 and 16 nm in the temperature range from 500 C to 1200 C, were synthesized by Nakaso et al (2003) in a quartz reactor.…”

Section: Morphologymentioning

confidence: 82%

“…They are commonly used as pigments and cosmetics, catalyst (Rekoske and Barteau 1997) or catalyst supports (Bankmann et al 1992), inorganic membranes (Gu and Oyama 2009), photocatalysts for waste water treatments (Ollis et al 1991), gas sensors (Ferroni et al 1996), dye-sensitized solar cells (Gr€ atzel 2003), and the surface coatings of paper (M€ akel€ a et al 2011). Recently, titania NPs were used for exposure experiments (Koivisto et al 2011). The National Institute for Occupational Safety and Health (NIOSH) recommends an airborne exposure limit of 2.4 mg/m 3 for respirable pigment-TiO 2 and 0.3 mg/m 3 for nano-TiO 2 as time weighted average concentrations for up to a 10 h per day during a 40-h work week over a whole life time (Koivisto et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Study of TiO₂ nanoparticle generation for follow-up inhalation experiments with laboratory animals

Moravec

Schwarz

Vodička

et al. 2016

Aerosol Science and Technology

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A method of long-lasting TiO 2 nanoparticle generation was tested for use in follow-up studies of the health impacts of nanoparticles on laboratory animals during several weeks' long exposure experiments. Nanoparticles were synthesized in an externally heated tube reactor by pyrolysis and oxidation of titanium tetraisopropoxide. Particle production was studied under varying reactor temperature, reactor flow rate, and precursor vapor pressure. A total of 264 h of particle generation were performed in four experimental campaigns using one batch of precursor without an observable decrease of particle production. As a result, particle production with number concentrations high above 1.0 £ 10 7 #/cm 3 and with primary particle sizes well below 50 nm could be achieved in most of the investigated experimental conditions. Maximum of particle mass concentration reached the value 9500 mg/m 3 , which corresponds with emission rate 29 mg/min. The dependence of nanoparticle production and characteristics on experimental conditions was evaluated and the most suitable parameters for exposure experiments were specified. A comparison of the results with data from the literature shows that the material of the reactor plays a significant role in the process of particle formation.

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“…A potential risk during product fabrication and handling is inhalation of the nanoparticles and hence a number of in vivo studies to investigate nanoparticle toxicity for this exposure route have been performed. These studies have been implemented using different types of particle administration such as inhalation (Tran et al 2000; Bermudez et al 2004; Grassian et al 2007; Donaldson et al 2008; Yang et al 2008; Koivisto et al 2011) and instillation/aspiration (Miyabara et al 1998; Tsurudome et al 1999; Jacobsen et al 2009). As a complement, in vitro studies of the nanoparticle interactions with lung cells have been carried out (Tippe et al 2002; Blank et al 2006; Pal et al 2006; Gaschen et al 2010; Ji et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Gas-borne particles with tunable and highly controlled characteristics for nanotoxicology studies

et al. 2012

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For nanotoxicology investigations of air-borne particles to provide relevant results it is ever so important that the particle exposure of, for example cells, closely resembles the “real” exposure situation, that the dosimetry is well defined, and that the characteristics of the deposited nanoparticles are known in detail. By synthesizing the particles in the gas-phase and directly depositing them on lung cells the particle deposition conditions in the lung is closely mimicked. In this work we present a setup for generation of gas-borne nanoparticles of a variety of different materials with highly controlled and tunable particle characteristics, and demonstrate the method by generation of gold particles. Particle size, number concentration and mass of individual particles of the population are measured on-line by means of differential mobility analyzers (DMA) and an aerosol particle mass analyzer (APM), whereas primary particle size and internal structure are investigated by transmission electron microscopy. A method for estimating the surface area dose from the DMA-APM measurements is applied and we further demonstrate that for the setup used, a deposition time of around 1 h is needed for deposition onto cells in an air–liquid interface chamber, using electrostatic deposition, to reach a toxicological relevant surface area dose.

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Aerosol characterization and lung deposition of synthesized TiO2 nanoparticles for murine inhalation studies

Cited by 9 publications

References 44 publications

Low doses of TiO₂-polyethylene glycol nanoparticles stimulate proliferation of hepatocyte cells

Low doses of TiO₂-polyethylene glycol nanoparticles stimulate proliferation of hepatocyte cells

Study of TiO₂ nanoparticle generation for follow-up inhalation experiments with laboratory animals

Gas-borne particles with tunable and highly controlled characteristics for nanotoxicology studies

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Aerosol characterization and lung deposition of synthesized TiO2 nanoparticles for murine inhalation studies

Cited by 9 publications

References 44 publications

Low doses of TiO2-polyethylene glycol nanoparticles stimulate proliferation of hepatocyte cells

Low doses of TiO2-polyethylene glycol nanoparticles stimulate proliferation of hepatocyte cells

Study of TiO2 nanoparticle generation for follow-up inhalation experiments with laboratory animals

Gas-borne particles with tunable and highly controlled characteristics for nanotoxicology studies

Contact Info

Product

Resources

About

Low doses of TiO₂-polyethylene glycol nanoparticles stimulate proliferation of hepatocyte cells

Low doses of TiO₂-polyethylene glycol nanoparticles stimulate proliferation of hepatocyte cells

Study of TiO₂ nanoparticle generation for follow-up inhalation experiments with laboratory animals