Aeromonas veronii, a potential pathogen of enteritis in snakehead fish Ophiocephalus argus

Abstract

Enteritis is known as a major disease in snakehead fish Ophiocephalus argus aquaculture and has resulted in large economic losses. Yet only scarce information is available on Aeromonas veronii as a causal agent for enteritis in O. argus. In this study, a virulent strain, temporarily named HY2, was isolated from diseased snakehead fish suffering from enteritis, and was identified as A. veronii through molecular and phenotypic methods. In addition, the HY2 isolate showed an LD50 value of 2.8×10 5 CFU mL-1 , and was highly sensitive to aminoglycosides, macrolides, polypeptides, quinolones, sulfonamides and tetracyclines antibiotics. To the best of our knowledge, this is the first report of A. veronii as a potential pathogen of enteritis in snakehead fish.

Bacillus cereus, a potential pathogen of snakehead fish Ophiocephalus argus

“…The potential pathogens were examined according to Wang et al (2020). First, the organs (liver, kidney, intestine, muscle, and gill) were dissected from diseased snakehead fish in the laboratory (Gu et al, 2019), compressed manually between two glass slides to prepare thin sections of the organs, and then subjected to a careful observation for potential parasites using light microscopy (Yang, 2018).…”

“…In particular, by implementing modern farming techniques, snakehead fish aquaculture in China has become a rapidly growing industry with a total production of over 548 kilotons in 2021 (Ministry of Agriculture and Rural Affairs of China, 2022). However, under intensive culture conditions, this industry has been badly contaminated with bacterial pathogens (Gu et al, 2019;Wang et al, 2020). The association of Aeromonas schubertii, A. veronii, Citrobacter freundii, and Nocardia seriolae with massive mortalities in snakehead fish has been well documented (Wang et al, 2007;Liu et al, 2012;Gu et al, 2019;Wang et al, 2020).…”

“…However, under intensive culture conditions, this industry has been badly contaminated with bacterial pathogens (Gu et al, 2019;Wang et al, 2020). The association of Aeromonas schubertii, A. veronii, Citrobacter freundii, and Nocardia seriolae with massive mortalities in snakehead fish has been well documented (Wang et al, 2007;Liu et al, 2012;Gu et al, 2019;Wang et al, 2020). Thus, bacterial pathogens in snakehead fish should be concerned.…”

Identification of Vibrio ponticus as a bacterial pathogen of coral trout Plectropomus leopardus

“…The potential pathogens were examined according to the previously described method (Wang et al, 2020). Firstly, the organs (liver, kidney, intestine, muscle, and gill) were dissected from the diseased coral trout in the laboratory, compressed manually between two glass slides to prepare the thin sections of organs, and then subjected to a observation for potential parasites using microscopy (Yang, 2018).…”