Aerofloral investigation and allergenic potentials of two dominant airborne pollen types at selected sites in South-western Nigeria

Adekanmbi, Olusola Helen; Alebiosu, Olugbenga Shadrak; Adeiga, A. A.

doi:10.1007/s10453-018-9533-7

Cited by 4 publications

(21 citation statements)

References 31 publications

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“…Polliniferous specimens were crushed using laboratory acidi ed sand sprinkled on the pollen grains, with the aid of a mortar and pestle, while 2 ml of buffer solution (phosphate buffered saline at pH 7.4) was added to ensure homogeneity of the specimens in the centrifuge tubes. Another 8 ml of buffer was used to rinse the remaining homogenate and was further introduced into the same set of centrifuge tubes (Adekanmbi et al 2018b;Alebiosu and Adekanmbi, 2022). Sample centrifugation was done at 5000 r.p.m for 10 minutes.…”

Section: Crude Pollen Protein Extraction and Assaymentioning

confidence: 99%

“…Consequently, the Mus musculus mice were used, while their nomenclature was authenticated by Dr. Nnamdi Amaeze of the Department of Zoology, University of Lagos, Lagos, Nigeria. Female Mus musculus PWK/PhJ (6 weeks old), were sourced and maintained under laboratory conditions in the Animal House, College of Medicine, University of Lagos, Idi-Araba, Lagos, Nigeria, following the procedure of Conejero et al (2007), Adekanmbi et al (2018b), Alebiosu and Adekanmbi (2022).…”

Section: Identi Cation and Sensitization Of Experimental Micementioning

confidence: 99%

“…The mice were subjected to all experimental protocols, according to international standards of animal welfare; research ethical approval (Protocol number IRB/15/302) was collected from the Institutional Review Board of the Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria (Adekanmbi et al 2018b;Alebiosu and Adekanmbi, 2022).…”

Section: Identi Cation and Sensitization Of Experimental Micementioning

confidence: 99%

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Aerobiological investigation, meteorological impacts on pollen distribution and allergenicity of major pollen types in the atmosphere of Southeastern Nigeria

Alebiosu

Adekanmbi

2022

Preprint

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Airborne palynomorphs are regarded as important organic granules causing human allergic reactions such as pollinosis and asthma. In the present study, monthly depositional amount of airborne pollen and fern spores, impact of the surrounding plants on pollen distribution and allergenicity of common airborne pollen types, were investigated at selected sampling stations in Ebonyi and Anambra States, Southeastern Nigeria. Bioaerosols were collected with Tauber-like pollen samplers and subjected to conventional palynological treatment processes, microscopy and photomicrography. Enumeration of plants within the immediate vegetation showed that local plants produced some atmospheric pollen types at the study locations. Among other meteorological parameters, only relative humidity was found to yield statistically significant and positive correlation with monthly total airborne pollen concentrations in Anambra State. A positive but not significant correlation was also found between total airborne pollen counts and relative humidity in Ebonyi State. Nephrolepis sp., Pteris sp. and a trilete fern produced spores, while diatoms were also recovered. Some common pollen types including those of Syzygium guineense (Willd.) DC. and Senna siamea (Lam.) Irwin et Barneby in Ebonyi State; Mariscus alternifolius Vahl. and Zea mays L. in Anambra State, were collected in order to quantify and extract their crude protein contents for Mus musculus sensitization to achieve serology (ELISA) and haematology (differential and total white blood cell counts). Statistical significance was tested and observed in the correlation between amounts of haematological and serological parameters yielded by each test group; variations between amounts of these parameters yielded by each test group and those of the control, as well as at differing periods of sensitization. In the Senna siamea test group of Mus musculus, histopathological morbid characteristics were observed, including areas of necrosis (presence of dead cells) in the bronchi of a mortality; areas of necrosis and congestion in the bronchi and lungs of another mortality, respectively, after second sensitization. The study confirmed all the selected pollen types as potential sources of allergy at the study areas and revealed a multiplex of interplay among triggers of pollen allergy at different intervals of administration in mice, establishing a model of immunological reactions to varied pollen antigens in humans.

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Section: Crude Pollen Protein Extraction and Assaymentioning

confidence: 99%

Section: Identi Cation and Sensitization Of Experimental Micementioning

confidence: 99%

Section: Identi Cation and Sensitization Of Experimental Micementioning

confidence: 99%

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Aerobiological investigation, meteorological impacts on pollen distribution and allergenicity of major pollen types in the atmosphere of Southeastern Nigeria

Alebiosu

Adekanmbi

2022

Preprint

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“…The first goal is significant because pollinosis has an "annual periodicity, with symptoms usually occurring at the same time of the year, during pollination." 55 Pollen data collected from different localities from a few months to 1 year in Nigeria, 12,13,20,21,24,[26][27][28][49][50][51][52]56 suggest that some pollen and/or spore types, based on their abundance or otherwise, could be responsible for allergenic reactions among patients of hay fever. For instance, it has been reported that Poaceae grains have a high possibility of stimulating pollinosis reaction in patients, 26 but neither this has been tested nor there are any proofs of such relationships in Nigeria.…”

Section: Aeropalynology Research and Health Practitionersmentioning

confidence: 99%

“…36,37 More so, setbacks in allergenicity studies of potential pollen allergens in Nigeria include (i) inadequate understanding of phenological shifts among plant species 28 during collection of pollen samples; (ii) difficulty in accessing human subjects; and (iii) its accompanying complexities of ethical issues. Adekanmbi et al 27 cited the work of Conejero et al, 38 and stated that an important limitation in their study of allergenicity was the subcutaneous route of Mus musculus sensitization. This phenomenon varied from that of human sensitization and accompanied by probably differential immune responses and disease manifestation.…”

Section: Introductionmentioning

confidence: 99%

review of aeropalynology research in Nigeria

Ajikah

Alebiosu

Orijemie

et al. 2021

aei

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Background: Aeropalynology is a branch of palynology that studies the content of atmospheric pollen grains and spores. The amount, concentration, and distribution of these aerospora are influenced by the seasonal flowering of parent plants and variations in climatic conditions as well as local and regional variabilities. Atmospheric pollen grains and spores are diverse and have been identified as major biological particles that trigger immune cells to release inflammatory chemical mediators, inducing respiratory-linked and allergic conditions, such as polli-nosis, among susceptible individuals. Objective: The burden of these allergic conditions on patients, families, healthcare systems, and governments has risen globally, thereby affecting developing countries, including Nigeria, wherein the financial and infrastructural institutions are not effective enough to mitigate these challenges. Avoidance of allergenic aerospora is an effective mode of addressing pollinosis with its associated conditions. However, there is a need to ascertain the atmospheric quantity, diversity, and pattern of occurrence of allergenic pollen/spores. Results: In this paper, we reviewed published articles on aeropalynology in Nigeria with atten-tion to the design and duration of the study and the used equipment. We further investigated whether identification and quantification of allergy-causing palynomorphs was part of published articles’ foci. Conclusion: The availability of such data/information is crucial for reducing epidemiological uncertainties, enhancing the diagnosis of allergic conditions, and securing a robust set of mitigation strategies and/or effective treatment of these conditions in Nigeria.

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Relevance of aerobiological studies in Nigeria: a two-year aerospora record of Lagos

et al. 2021

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Aerofloral investigation and allergenic potentials of two dominant airborne pollen types at selected sites in South-western Nigeria

Cited by 4 publications

References 31 publications

Aerobiological investigation, meteorological impacts on pollen distribution and allergenicity of major pollen types in the atmosphere of Southeastern Nigeria

Aerobiological investigation, meteorological impacts on pollen distribution and allergenicity of major pollen types in the atmosphere of Southeastern Nigeria

review of aeropalynology research in Nigeria

Relevance of aerobiological studies in Nigeria: a two-year aerospora record of Lagos

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