Aerobic to anaerobic transition in Biomphalaria glabrata (Say, 1818) infected with different miracidial doses of Echinostoma paraensei (Lie and Basch, 1967) by high-performance liquid chromatography

Tunholi, Victor Menezes; Tunholi-Alves, Vinícius Menezes; Lustrino, Danilo; Castro, Rosane Nora; Sant’Ana, Luiza D‘Oliveira; Garcia, Juberlan Silva; Maldonado, Arnaldo; Santos, Marcos Antônio José dos; Rodrigues, Maria de Lurdes de Azevedo; Pinheiro, Jairo

doi:10.1016/j.exppara.2013.01.012

Cited by 28 publications

(6 citation statements)

References 27 publications

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“…The immortalization of Bge cells could also have occurred as a result of similar changes. Furthermore, it has been shown that infection of B. glabrata by various trematodes including S. mansoni [45–47], E. caproni [48], and E. paraensei [49] and the nematode Angiostrongylus cantonensis [50] greatly modifies the snail’s glucose metabolism and storage pathways. B. glabrata glycogen reserves decrease after A. cantonensi s and S. mansoni infection, and a significant reduction in the concentration of free glucose in the hemolymph is also a consequence of larval infections.…”

Section: Discussionmentioning

confidence: 99%

Sequence and structural variation in the genome of the Biomphalaria glabrata embryonic (Bge) cell line

et al. 2018

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BackgroundThe aquatic pulmonate snail Biomphalaria glabrata is a significant vector and laboratory host for the parasitic flatworm Schistosoma mansoni, an etiological agent for the neglected tropical disease schistosomiasis. Much is known regarding the host-parasite interactions of these two organisms, and the B. glabrata embryonic (Bge) cell line has been an invaluable resource in these studies. The B. glabrata BB02 genome sequence was recently released, but nothing is known of the sequence variation between this reference and the Bge cell genome, which has likely accumulated substantial genetic variation in the ~50 years since its isolation.ResultsHere, we report the genome sequence of our laboratory subculture of the Bge cell line (designated Bge3), which we mapped to the B. glabrata BB02 reference genome. Single nucleotide variants (SNVs) were predicted and focus was given to those SNVs that are most likely to affect the structure or expression of protein-coding genes. Furthermore, we have highlighted and validated high-impact SNVs in genes that have often been studied using Bge cells as an in vitro model, and other genes that may have contributed to the immortalization of this cell line. We also resolved representative karyotypes for the Bge3 subculture, which revealed a mixed population exhibiting substantial aneuploidy, in line with previous reports from other Bge subcultures.ConclusionsThe Bge3 genome differs from the B. glabrata BB02 reference genome in both sequence and structure, and these are likely to have significant biological effects. The availability of the Bge3 genome sequence, and an awareness of genomic differences with B. glabrata, will inform the design of experiments to understand gene function in this unique in vitro snail cell model. Additionally, this resource will aid in the development of new technologies and molecular approaches that promise to reveal more about this schistosomiasis-transmitting snail vector.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-3059-2) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Sequence and structural variation in the genome of the Biomphalaria glabrata embryonic (Bge) cell line

et al. 2018

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“…The glycolytic pathway is one of the major pathways by which animals obtain ATP through the catabolism of carbohydrates, and HK, PK and LDH are the key enzymes involved (Hochachka, Freed, Somero, & Prosser, ; Tunholi et al., ). After feeding with the crude In‐Poly extract in the present study, the activities of HK, PK, PFK and LDH in the hepatopancreas were higher than those in the Blank group.…”

Section: Discussionmentioning

confidence: 99%

Effects of crude extracellular protein and crude intracellular polysaccharides ofVibrio alginolyticuson the growth, energy metabolism regulation and WSSV resistance ofLitopenaeus vannamei

Shan

Liu

et al. 2018

Aquacult Nutr

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This study aimed to investigate the effects of adding crude extracts of the extracellular protein (Ex‐Pro) and intracellular polysaccharides (In‐Poly) of Vibrio alginolyticus to diets, at a dosage of 10 g/kg, on the growth, energy metabolism and WSSV (White Spot Syndrome Virus) resistance of Litopenaeus vannamei (initial weight, 0.88 ± 0.04 g/shrimp). Growth and survival rate were not significantly affected by the crude extracts (p > 0.05). Shrimp fed Ex‐Pro crude extracts showed higher succinate dehydrogenase (SDH) activity in the hepatopancreas and muscle but lower lactate dehydrogenase (LDH) activity in the muscle (p < 0.05). The activities of hexokinase (HK), pyruvate kinase (PK), LDH and SDH in the hepatopancreas and the activity of SDH in the muscle were significantly increased by feeding In‐Poly crude extracts (p < 0.05). In contrast, the content of fatty acid synthase (FAS) in the muscle was significantly reduced by the crude extracts (p < 0.05). The contents of glucose and triglyceride and the activity of the electron transport system in the hepatopancreas were significantly increased by the crude extracts (p < 0.05), and the WSSV resistance of the shrimp was increased. These results indicated that the Ex‐Pro and In‐Poly crude extracts of V. alginolyticus could affect energy metabolism, and there was a correlation between WSSV resistance and energy metabolism in L. vannamei.

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“…For this reason, the described increase in Ca 2+ in B. glabrata hemolymph when exposed to 20 mg/L CaCO 3 for fifteen days could be explained by the mobilization of this ion from the shell to the hemolymph to work as a buffering agent (Becker, 1980). The Ca 2+ is also important in the glycolytic and TCA metabolic pathways of pulmonate aquatic snails, as it acts as a cofactor in the activation of many enzymes (Tunholi et al, 2013;Sminia et al, 1980). Our findings indicate a reduction in the concentration of glucose in the hemolymph of snails exposed to 20 and 40 mg of CaCO 3 .…”

Section: Discussionmentioning

confidence: 99%

“…In aerobic conditions, it is possible to observe high concentrations of pyruvate, oxaloacetate, citrate, succinate, fumarate and malate in the snail hemolymph, which indicate the activation of the TCA cycle. In an alternative metabolic pathway, it is possible to find increased concentrations of ketone bodies, such as acetoacetate and beta-hydroxybutyrate, which are products of the lipid metabolism, while the increase in lactate, oxalate and propionate indicate anaerobic glycolytic processes (Bezerra et al, 1999;Tunholi et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Changes in energetic metabolism of Biomphalaria glabrata (Mollusca, Planorbidae) in response to exogenous calcium

et al. 2016

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Calcium is considered an essential element for the metabolism of aquatic snail Biomphalaria glabrata (Say, 1818), intermediate host of Schistosoma mansoni Sambon, 1907 in Brazil, and represents a limiting factor to its distribution and adaptation to the environment. This study investigated the effect of different concentrations of exogenous CaCO 3 on the energetic metabolism of B. glabrata for better understanding the physiological interference of chemical elements dissolved in the environment with the physiology of this species. Sixty-day-old snails were distributed into six groups, five exposed to different concentrations of CaCO 3 (20, 40, 60, 80 and 100 mg/L) and a control group. The exposure to CaCO 3 was assessed over time, with analysis of 15 snails of each group in the following intervals: 1, 14, 21 or 30 days for hemolymph extraction. Concentrations of calcium and glucose in the hemolymph were determined by commercial kits, and organic acids were extracted using an ion exchange column and analyzed by high-performance liquid chromatography. Concentration of calcium in the hemolymph showed no significant difference (p>0.05) from the control group and between the concentrations tested. Concentration of glucose decreased (p<0.05) in the treatments of exposure to 20 and 40 mg/L and increased when exposed to 80 and 100 mg/L CaCO 3 compared to control and to other concentrations tested over 30 days. The organic acids pyruvate, oxaloacetate, citrate, succinate, fumarate, beta-hydroxybutyrate and lactate presented increased concentrations, while propionate and acetoacetate, decreased concentrations, when exposed to CaCO 3 compared to control. Considering the influence of different periods of exposure to CaCO 3 , on the 14 th day, there were stronger alterations in the metabolism of B. glabrata. In conclusion, exposure to CaCO 3 reduced the concentration of glucose, which is metabolized into pyruvate, the final product of glycolysis, and also influenced the energetic metabolism pathways, indicating an aerobic or partially anaerobic functioning.Keywords: snails, Krebs cycle, CaCO 3 exposure, energetic metabolism. (20, 40, 60, 80 e 100mg/L) e um controle. A exposição ao CaCO 3 foi avaliada em função do tempo, sendo retirados 15 moluscos de cada grupo nos seguintes intervalos: 1, 14, 21 ou 30 dias para extração da hemolinfa. As concentrações de cálcio e glicose na hemolinfa foram determinadas usando-se kits comercial e os ácidos orgânicos foram extraídos por meio da coluna de troca iônica e analisados através cromatografia líquida Alterações no metabolismo energético deBraz.

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Aerobic to anaerobic transition in Biomphalaria glabrata (Say, 1818) infected with different miracidial doses of Echinostoma paraensei (Lie and Basch, 1967) by high-performance liquid chromatography

Cited by 28 publications

References 27 publications

Sequence and structural variation in the genome of the Biomphalaria glabrata embryonic (Bge) cell line

Sequence and structural variation in the genome of the Biomphalaria glabrata embryonic (Bge) cell line

Effects of crude extracellular protein and crude intracellular polysaccharides ofVibrio alginolyticuson the growth, energy metabolism regulation and WSSV resistance ofLitopenaeus vannamei

Changes in energetic metabolism of Biomphalaria glabrata (Mollusca, Planorbidae) in response to exogenous calcium

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