Aerobic methanotrophic communities at the Red Sea brine-seawater interface

Abdallah, Rehab Z.; Adel, Mustafa; Ouf, Amged; Sayed, Ahmed A.; Ghazy, Mohamed A.; Álam, Intikhab; Essack, Magbubah; Lafi, Feras F.; Bajic, Vladimir B.; El-Dorry, Hamza A.; Siam, Rania

doi:10.3389/fmicb.2014.00487

Cited by 30 publications

(44 citation statements)

References 73 publications

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“…Similar to findings from hypoxic Baltic Sea waters [35], this suggests that MOB at Kryos live at the very edge of their habitable ecological niche. At the interface of thalassohaline brines, aerobic methanotrophy was already evidenced directly or indirectly [66][67][68]. With this study, we demonstrate for the first time that MOB can also adapt to microoxic conditions in an athalassohaline brine.…”

Section: Methanotrophy In the Interfacesupporting

confidence: 52%

Life on the edge: active microbial communities in the Kryos MgCl2-brine basin at very low water activity

et al. 2018

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The Kryos Basin is a deep-sea hypersaline anoxic basin (DHAB) located in the Eastern Mediterranean Sea (34.98°N 22.04°E). It is filled with brine of re-dissolved Messinian evaporites and is nearly saturated with MgCl-equivalents, which makes this habitat extremely challenging for life. The strong density difference between the anoxic brine and the overlying oxic Mediterranean seawater impedes mixing, giving rise to a narrow chemocline. Here, we investigate the microbial community structure and activities across the seawater-brine interface using a combined biogeochemical, next-generation sequencing, and lipid biomarker approach. Within the interface, we detected fatty acids that were distinctly C-enriched when compared to other fatty acids. These likely originated from sulfide-oxidizing bacteria that fix carbon via the reverse tricarboxylic acid cycle. In the lower part of the interface, we also measured elevated rates of methane oxidation, probably mediated by aerobic methanotrophs under micro-oxic conditions. Sulfate reduction rates increased across the interface and were highest within the brine, providing first evidence that sulfate reducers (likely Desulfovermiculus and Desulfobacula) thrive in the Kryos Basin at a water activity of only ~0.4 A. Our results demonstrate that a highly specialized microbial community in the Kryos Basin has adapted to the poly-extreme conditions of a DHAB with nearly saturated MgCl brine, extending the known environmental range where microbial life can persist.

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Section: Methanotrophy In the Interfacesupporting

confidence: 52%

Life on the edge: active microbial communities in the Kryos MgCl2-brine basin at very low water activity

et al. 2018

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“…In this study, 25.2% of the Pseudomonadales was not affiliated with a known family. Unknown phylotypes of Phyllobacterium (Phyllobacteriaceae) dominated in the Red Sea brine-seawater interface (54%) (Abdallah et al, 2014), and the Phyllobacteriaceae represented 9.1% in this study. Phylotypes belonging to Phyllobacterium species participate in aromatic hydrocarbon mineralization and have been retrieved from marine ecosystems (von der Weid et al, 2008; Shao et al, 2010).…”

Section: Discussionmentioning

confidence: 47%

Reducing Salinity by Flooding an Extremely Alkaline and Saline Soil Changes the Bacterial Community but Its Effect on the Archaeal Community Is Limited

et al. 2017

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Regular flooding of the soil to reduce salinity will change soil characteristics, but also the microbial community structure. Soil of the former lake Texcoco with electrolytic conductivity (EC) 157.4 dS m-1 and pH 10.3 was flooded monthly in the laboratory under controlled conditions for 10 months while soil characteristics were determined and the archaeal and bacterial community structure monitored by means of 454 pyrosequencing of the 16S rRNA gene. The EC of the soil dropped from 157.8 to 1.7 dS m-1 and the clay content decreased from 430 to 270 g kg-1 after ten floodings, but the pH (10.3) did not change significantly over time. Flooding the soil had a limited effect on the archaeal community structure and only the relative abundance of Haloferax-like 16S rRNA phylotypes changed significantly. Differences in archaeal population structure were more defined by the initial physicochemical properties of the soil sample than by a reduction in salinity. Flooding, however, had a stronger effect on bacterial community structure than on the archaeal community structure. A wide range of bacterial taxa was affected significantly by changes in the soil characteristics, i.e., four phyla, nine classes, 17 orders, and 28 families. The most marked change occurred after only one flooding characterized by a sharp decrease in the relative abundance of bacterial groups belonging to the Gammaproteobacteria, e.g., Halomonadaceae (Oceanospirillales), Pseudomonadaceae, and Xanthomonadaceae and an increase in that of the [Rhodothermales] (Bacteroidetes), Nitriliruptorales (Actinobacteria), and unassigned Bacteria. It was found that flooding the soil sharply reduced the EC, but also the soil clay content. Flooding the soil had a limited effect on the archaeal community structure, but altered the bacterial community structure significantly.

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“…The Atlantis II area includes the largest brine pool Atlantis II (ATIID), Discovery Deep (DD) and Chain Deep (CD)8. Oxygen levels are low in ATIID and DD, the subjects of this study, measuring <1 ml/l below 2000 m depth, but Oxygen is fully absent at higher temperatures-in the ATIID lower brine layer3910. Sources of light are scarce and limited to bioluminescence as the deeps are well beyond the photic zone11.…”

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confidence: 86%

Viruses-to-mobile genetic elements skew in the deep Atlantis II brine pool sediments

Adel

Elbehery

Aziz

et al. 2016

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The central rift of the Red Sea has 25 brine pools with different physical and geochemical characteristics. Atlantis II (ATIID), Discovery Deeps (DD) and Chain Deep (CD) are characterized by high salinity, temperature and metal content. Several studies reported microbial communities in these brine pools, but few studies addressed the brine pool sediments. Therefore, sediment cores were collected from ATIID, DD, CD brine pools and an adjacent brine-influenced site. Sixteen different lithologic sediment sections were subjected to shotgun DNA pyrosequencing to generate 1.47 billion base pairs (1.47 × 109 bp). We generated sediment-specific reads and attempted to annotate all reads. We report the phylogenetic and biochemical uniqueness of the deepest ATIID sulfur-rich brine pool sediments. In contrary to all other sediment sections, bacteria dominate the deepest ATIID sulfur-rich brine pool sediments. This decrease in virus-to-bacteria ratio in selected sections and depth coincided with an overrepresentation of mobile genetic elements. Skewing in the composition of viruses-to-mobile genetic elements may uniquely contribute to the distinct microbial consortium in sediments in proximity to hydrothermally active vents of the Red Sea and possibly in their surroundings, through differential horizontal gene transfer.

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Aerobic methanotrophic communities at the Red Sea brine-seawater interface

Cited by 30 publications

References 73 publications

Life on the edge: active microbial communities in the Kryos MgCl2-brine basin at very low water activity

Life on the edge: active microbial communities in the Kryos MgCl2-brine basin at very low water activity

Reducing Salinity by Flooding an Extremely Alkaline and Saline Soil Changes the Bacterial Community but Its Effect on the Archaeal Community Is Limited

Viruses-to-mobile genetic elements skew in the deep Atlantis II brine pool sediments

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