Aerobic Metabolism of Trichloroethylene by a Bacterial Isolate

Nelson, Michael J.; Montgomery, Stacy O.; O'Neill, Ellen J.; Pritchard, P. H.

doi:10.1128/aem.52.2.383-384.1986

Cited by 172 publications

(74 citation statements)

References 6 publications

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“…Strain 31A was isolated from a metal-contaminated industrial site and is resistant to higher levels of Ni (20 mM) and Co (20 mM), but lower levels of Zn (10 mM) and Cd (1 mM) [39], compared to strain CH34. Burkholderia cepacia G4, the parent strain of PR1, was isolated from a holding pond at an industrial waste-processing facility contaminated with organochlorine compounds [1].…”

Section: Differences In Ni-tolerance Mechanismsmentioning

confidence: 99%

“…Trichloroethylene (TCE), a common contaminant at waste sites, is found at 62% (1,021 sites) of the U. S. Environmental Protection Agency's (U.S. EPA) 1,636 National Priority List sites (www.atsdr.cdc.gov/supportdocs/ appendix-a.pdf). Burkholderia cepacia G4 (G4) was the first aerobic microorganism isolated in pure culture capable of degrading TCE [1]. Degradation of TCE is a cometabolic event, requiring the presence of growth substrates such as toluene or phenol to induce the production of enzymes necessary for degradation.…”

Section: Introductionmentioning

confidence: 99%

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Effect of pH on the toxicity of nickel and other divalent metals to Burkholderia cepacia PR1₃₀₁

Nostrand

Sowder

Bertsch

et al. 2005

Enviro Toxic and Chemistry

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Nickel (Ni) is a common cocontaminant at many waste sites where the soils and sediments often are acidic, thereby influencing metal availability. Growth of Burkholderia cepacia PR1(301) was not affected at 3.41 mM Ni at pH 5, but was inhibited by 73.2% at pH 6 and inhibited completely at pH 7 compared to growth without Ni. This pH effect was not observed in the Ni-resistant strains, Ralstonia metallidurans CH34 and 31A. Predicted Ni speciation did not explain the observed toxicity trends. Sorption of Ni to PR1 increased with increasing pH (1.49, 1.12, and 3.88 mg Ni/g dry weight at pH 5, 6, and 7, respectively), but was low at all three pH values, and most likely does not explain the observed pH effect. Growth inhibition of PRI with increasing pH also was observed for other divalent cations, with growth observed at 4.24 mM Co, 2.22 mM Cd, and 3.82 mM Zn at pH 5 and 6, but totally inhibited at pH 7. These studies suggest that, at circumneutral pH, PRI would be considered sensitive to Ni and other divalent cations, in spite of the ability to grow in higher concentrations at lower pH values.

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Section: Differences In Ni-tolerance Mechanismsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of pH on the toxicity of nickel and other divalent metals to Burkholderia cepacia PR1₃₀₁

Nostrand

Sowder

Bertsch

et al. 2005

Enviro Toxic and Chemistry

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show abstract

“…Aerobic degradation of TCE has been demonstrated by many different oxygenases. Toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4, for example, has been shown to oxidize TCE to primarily CO 2 and Cl 2 (Nelson et al, 1986). In addition, TOM can degrade other reductivedehalogenation products of TCE such as cis-DCE and trans-DCE (Canada et al, 2002;Shim et al, 2000), making TOM a promising candidate for bioremediation of TCE.…”

Section: Introductionmentioning

confidence: 99%

Engineering TCE-degrading rhizobacteria for heavy metal accumulation and enhanced TCE degradation

Lee

Wood

Chen

2006

Biotechnol. Bioeng.

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Many superfund sites are currently co-contaminated with organic pollutants such as trichloroethene (TCE) and heavy metals. A promising strategy to address these mixed-waste situations is the use of TCE-degrading rhizobacteria that will survive and thrive in soil heavily polluted with heavy metals. In this work, a gene coding for the metal-binding peptide, EC20, was introduced into rhizobacteria engineered for TCE degradation, resulting in strains with both metal accumulation and TCE degradation capabilities. EC20 was displayed onto the cell surface of Pseudomonas strain Pb2-1 and Rhizobium strain 10320D using an ice-nucleation protein (INP) anchor. Expression of EC20 was confirmed by Western blot analysis and cells with EC20 expression showed sixfold higher cadmium accumulation than non-engineered strains in the presence of 16 microM CdCl(2). As expected, the TCE degradation rate was reduced in the presence of cadmium for cells without EC20 expression. However, expression of EC20 (higher cadmium accumulation) completely restored the level of TCE degradation. These results demonstrated that EC20 expression enhanced not only cadmium accumulation but also reduced the toxic effect of cadmium on TCE degradation. We expect that similar improvements will be observed when these engineered rhizobacteria are inoculated onto plant roots.

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“…Pseudomonas strains used for phage testing were P. fluorescens Pf0-1 (Deflaun et al, 1994) and ATCC27663; Pseudomonas putida F1 (Gibson et al, 1968), BG1 (Whited et al, 1986), F39/D (Gibson et al, 1970) and ATCC12633; Pseudomonas oleovorans ATCC29347; Pseudomonas stutzeri C250 (Resnick, 1997); and Pseudomonas sp. strain 9816-11 (Torok et al, 1995), 9816-4 (Serdar & Gibson, 1989) and CA10W10 (Sato et al, 1997); Pseudomonas cepacia (Nelson et al, 1986). Cells were grown in animal product-free soy hydrolysate medium [an Luria-Bertani (LB) broth substitute] (Teknova, Hollister, CA), adding 250 mg mL À1 uracil if needed, and incubated at 30 1C (P. fluorescens) or 37 1C (E. coli).…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Characterization of the SOS response of Pseudomonas fluorescens strain DC206 using whole-genome transcript analysis

Jin

Retallack

Stelman

et al. 2007

FEMS Microbiology Letters

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DNA microarray technology was used to survey changes in gene expression in Pseudomonas fluorescens after mitomycin C treatment. As expected, genes associated with the SOS response were upregulated, such as those encoding the recombination protein RecA, DNA repair protein RecN, excinuclease ABC subunit A UvrA, and the LexA repressor protein. Interestingly, expression of 33 clustered bacteriophage-like genes was upregulated, suggesting that mitomycin C (MMC) may induce a prophage resident in the P. fluorescens genome. However, no phage particles were detected in P. fluorescens strain DC206 that had been treated with MMC using transmission electron microscopy. The same preparation failed to produce phage plaques on lawns of any of 10 different Pseudomonas strains tested, indicating that the 33 bacteriophage-like gene cluster represents a defective prophage.

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Aerobic Metabolism of Trichloroethylene by a Bacterial Isolate

Cited by 172 publications

References 6 publications

Effect of pH on the toxicity of nickel and other divalent metals to Burkholderia cepacia PR1₃₀₁

Effect of pH on the toxicity of nickel and other divalent metals to Burkholderia cepacia PR1₃₀₁

Engineering TCE-degrading rhizobacteria for heavy metal accumulation and enhanced TCE degradation

Characterization of the SOS response of Pseudomonas fluorescens strain DC206 using whole-genome transcript analysis

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Aerobic Metabolism of Trichloroethylene by a Bacterial Isolate

Cited by 172 publications

References 6 publications

Effect of pH on the toxicity of nickel and other divalent metals to Burkholderia cepacia PR1301

Effect of pH on the toxicity of nickel and other divalent metals to Burkholderia cepacia PR1301

Engineering TCE-degrading rhizobacteria for heavy metal accumulation and enhanced TCE degradation

Characterization of the SOS response of Pseudomonas fluorescens strain DC206 using whole-genome transcript analysis

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Effect of pH on the toxicity of nickel and other divalent metals to Burkholderia cepacia PR1₃₀₁

Effect of pH on the toxicity of nickel and other divalent metals to Burkholderia cepacia PR1₃₀₁