Aerobic L-tartrate Utilization by Bacillus Isolates

Abstract

Citation: Disha Patel and Aditi Buch, Aerobic L-tartrate Utilization by Bacillus Isolates, J Pure Appl Microbiol., 2019; 13(4):2045-2054. https://doi.org/10.22207/JPAM.13.4.16 Abstract Microbial utilization of uncommon C 4 dicarboxylate L-tartrate is largely anaerobic, with aerobic L-tartrate utilization known for few bacterial species including Rhodopseudomonas sphaeroides and Pseudomonas putida. Aerobic L-tartrate-utilizing microbes could be industrially relevant owing to the efficient nature of the bioprocess and catalytic versatility of tartrate dehydrogenase (TDH) responsible for aerobic catabolism of L-tartrate. Present work involves isolation and characterization of Bacillus strains capable of aerobic L-tartrate utilization and its correlation with occurrence of TDH activity. Two out of 37 isolates, IC1-G and IC1-Y were identified as Bacillus megaterium spp. showing efficient aerobic growth, utilizing ~3.7 and 2.8 mM L-tartrate respectively at the end of 48 h. Several organic acids possibly including oxalic, succinic and citric acids were secreted as by-products of L-tartrate metabolism. Utilization of L-tartrate directly correlated with induction of TDH activity by ~3.2 and 5.2 folds in IC1-G and IC1-Y respectively, when grown in presence of L-tartrate as compared to when grown on citrate. Overall, this study contributes Bacillus as only the third genus capable of aerobic, TDH mediated L-tartrate utilization. These Bacillus isolates thus offer potential targets to develop an industrially relevant bioprocess and biocatalyst.

Synthesis, Characterization and Anticancer Activity of 5-Substituted 4,5,6,7-Tetrahydro- N-(tetrahydro-2H-pyran-4-yl)thieno[3,2-c]pyridine-2-carboxamide Derivatives

“…4,5,6,7-Tetrahydrothieno-pyridine (THTP) represents a widely used lead structure with multitude of interesting applications in numerous pharmacological fields. Thus, various biological activities such as antimicrobial activity [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15], antileishmanial activity [16], antiarrhythmic activity [17], antiinflammatory activity [18][19][20][21][22], antihyperlipidemic activity [23], antidepressant activity [24], anticancer activity [25,26], antiplatelet activity [27,28], antidiabetic [29][30][31] and antituberculosis activity [32,33] have been reported and explored till date. Fusion of other nuclei like benzene, indole, oxadiazole, triazole rings to THTP nucleus enhances the pharmacological activities than its parent nucleus.…”