Aerobic Denitrification Microbial Community and Function in Zero-Discharge Recirculating Aquaculture System Using a Single Biofloc-Based Suspended Growth Reactor: Influence of the Carbon-to-Nitrogen Ratio

Deng, Min; Dai, Zhili; Yeerken, Senbati; Li, Lü; Song, Kang; He, Xinyi

doi:10.3389/fmicb.2020.01760

Cited by 40 publications

(22 citation statements)

References 49 publications

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“…NapA gene coding for periplasmic nitrate reductase is responsible for aerobic denitrification and which could be active at DO of 3-5 mg L −1 , pH 7-8 and temperature 25-37°C in aerobic reactors (Ji et al 2015). Recently, around 75% of N was found to be removed as gas by aerobic denitrifiers such as Flavobacterium, Dechloromonas, Rhodobacter and Zoogloea using napA, nirK and nosZ genes in zero-discharge biofloc-based recirculating aquaculture systems (Deng et al 2020). On analysing the bacterial community diversity of nitrifying-denitrifying biofilters in marine RAS bioreactors, ammonia and nitrite oxidizers were found to be more abundant in nitrification biofilter and autotrophic and heterotrophic denitrifiers in denitrifying biofilter and were more likely to be originated from the water source provided (Brailo et al 2019).…”

Section: Anaerobic Nitrificationmentioning

confidence: 99%

Nitrification and denitrification in recirculating aquaculture systems: the processes and players

Preena

Kumar

Singh

2021

Reviews in Aquaculture

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One of the major challenges in any sustainable aquaculture production systems is the accumulation of nitrogenous waste such as ammonia and its biological nitrification products viz nitrite and nitrate. Considering the bio-security issues, amelioration of these wastes without water exchange can be accomplished only by way of establishing in situ nitrification and denitrification through biofilters/ bioreactors activated with nitrifying/denitrifying bioaugmentors. In such systems, coexistence of aerobic denitrifiers, anaerobic ammonia oxidizers (Anammox) and complete ammonia oxidizers (Comammox) together with the autotrophic nitrifiers enhance the coupled nitrification-denitrification. This promotes total nitrogen removal without external carbon supplements or additional anerobic compartment in the system. Various recirculating aquaculture systems (RAS) comprise diverse nitrifying community in biofilters/bioreactors thereby imparting distinctive nitrogen conversion in the system. Meanwhile, the structure and population dynamics of the nitrifying/denitrifying consortia are influenced by the environmental factors forming the decisive factors of the success of the processes. Accordingly, understanding the complexity of nitrifying/denitrifying community composition turns out to be a requirement to facilitate its improvised performance. In this context, the review addresses different biological nitrogen removal systems, significance of nitrification-denitrification in RAS, genetic diversity of the key players in RAS, methods of analysis of their community structure, current application and prospects of the processes in RAS.

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Section: Anaerobic Nitrificationmentioning

confidence: 99%

Nitrification and denitrification in recirculating aquaculture systems: the processes and players

Preena

Kumar

Singh

2021

Reviews in Aquaculture

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“…2015; Deng et al . 2020). Previously, it was believed that the denitrifying enzymes are sensitive to oxygen and that, the denitrification occurred only under anaerobic conditions in presence of nitrate as the final electron acceptor while the role of electron acceptor in the co‐respiration mode was performed by oxygen and nitrate in the oxic group of micro‐organisms (Huang and Tseng 2001).…”

Section: Snd Pathway: a New Strategy Adopted By Microbial Communities For Nitrogen Removalmentioning

confidence: 99%

“…2013; Deng et al . 2020). Such a denitrification under aerobic conditions is termed as ‘microzones denitrification’, which can occur at very low DO concentrations simultaneously as terminal electron acceptors.…”

Section: Mechanisms Of Snd Pathwaymentioning

confidence: 99%

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Role of heterotrophic nitrifiers and aerobic denitrifiers in simultaneous nitrification and denitrification process: a nonconventional nitrogen removal pathway in wastewater treatment

Gupta

Poddar

Nakhate

et al. 2021

Letters Applied Microbiology

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Bacterial species capable of performing both nitrification and denitrification in a single vessel under similar conditions have gained significance in the wastewater treatment scenario considering their unique character of performing the above reactions under heterotrophic and aerobic conditions respectively. Such a novel strategy often referred to as simultaneous nitrification and denitrification (SND) has a tremendous potential in dealing with various wastewaters having low C : N content, considering that the process needs very little or no external carbon source and oxygen supply thus adding to its cost‐effective and environmentally friendly nature. Though like other micro‐organisms, heterotrophic nitrifiers and aerobic denitrifiers convert inorganic or organic nitrogen‐containing substances into harmless dinitrogen gas in the wastewater, their ecophysiological role in the global nitrogen cycle is still not fully understood. Attempts to highlight the role played by the heterotrophic nitrifiers and aerobic denitrifiers in dealing with nitrogen pollution under various environmental operating conditions will help in developing a mechanistic understanding of the SND process to address the issues faced by the traditional methods of aerobic autotrophic nitrification–anaerobic heterotrophic denitrification.

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“…Previous researches have attempted to control suspended solid levels in the conventional BFT systems within a desirable range, but the difficulty of operation, high level of inorganic nitrogen concentration, and unstable water parameters (e.g., pH and alkalinity) remain major challenges 9,15,18,19 . Recently, a modified BFT system, which segregates the culture tank from the solid settling tank, was proposed for suspended solid control 20,21 . Although the settling tank was capable of maintaining acceptable levels of inorganic nitrogen compounds and suspended solids, it was more susceptible to the resuspension of settled solids when the BFT systems operated at high water flow rate and with intensive aeration to ensure good liquid mixing and sufficient oxygen in the culture tank.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of modified biofloc system with filtration unit in controlling suspended solids and inorganic nitrogen concentrations in a recirculating aquaculture system

Kunwong

Satanwat

Powtongsook

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND Biofloc technology systems (BFT) often encounter difficulties in controlling suspended solid levels in tanks. The oxygen consumption rates and ammonia removal rates of bioflocs as the BFT system shifts from assimilation towards nitrification have rarely been reported. Thus, the performance of the BFT system with a filtration unit was evaluated. RESULTS Preliminary work found that the optimal screen pore size and recirculating flow rate were 100 μm and 3000 L day−1, respectively. The BFT system with the filtration unit could maintain suspended solids in the fish tank below 50 mg‐SS L−1 while retaining significantly higher suspended solids in the biofloc reactor (509 ± 44.6 mg‐SS L−1). The daily starch addition at a C:N mass ratio of 10:1 in phase I (day 1–38) induced the biofloc formation that kept TAN and nitrite levels below 1.0 mg‐N L−1. The fraction of inorganic nitrogen compounds increased from 2.5% in phase I to 27.6% in phase III (day 56–70). TAN removal rates increased from 3.0 mg‐N g‐SS−1 day−1 during phase I to 10.34 ± 0.392 mg‐N g‐SS−1 day−1 after complete nitrification occurred. Relatively constant oxygen consumption rates of the bioflocs (149.1 ± 9.53 mg‐ O2 g‐SS−1 day−1) were observed for the entire experiment. CONCLUSION The BFT system with a filtration unit was effective in controlling suspended solid and inorganic nitrogen concentrations. Nitrogen treatment pathway of BFT system was assimilation during startup period but gradually shifted to nitrification. Oxygen consumption rates of the bioflocs are relatively constant regardless of the pathways responsible for nitrogen treatment. © 2021 Society of Chemical Industry (SCI).

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Aerobic Denitrification Microbial Community and Function in Zero-Discharge Recirculating Aquaculture System Using a Single Biofloc-Based Suspended Growth Reactor: Influence of the Carbon-to-Nitrogen Ratio

Cited by 40 publications

References 49 publications

Nitrification and denitrification in recirculating aquaculture systems: the processes and players

Nitrification and denitrification in recirculating aquaculture systems: the processes and players

Role of heterotrophic nitrifiers and aerobic denitrifiers in simultaneous nitrification and denitrification process: a nonconventional nitrogen removal pathway in wastewater treatment

Evaluation of modified biofloc system with filtration unit in controlling suspended solids and inorganic nitrogen concentrations in a recirculating aquaculture system

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