Aerobic degradation of a hydrocarbon mixture in natural uncontaminated potting soil by indigenous microorganisms at 20 °C and 6 °C

Eriksson, Mikael; Dalhammar, Gunnel; Borg‐Karlson, Anna‐Karin

doi:10.1007/s002530051429

Cited by 46 publications

(27 citation statements)

References 14 publications

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“…This might be due to the maintenance of suitable soil conditions, including moisture level, nutrients, and aeration. Thus, the high rate of bioremediation observed during the study was in fact due to the survival of the selected bacterial consortium under field conditions (3,11).…”

Section: Discussionmentioning

confidence: 99%

“…Many of the constituents of oily sludge are carcinogenic and potent immunotoxicants (24). Among the many techniques employed to decontaminate the affected sites, in situ bioremediation using indigenous microorganisms is by far the most widely used (3,9,11,12,21,30). This approach to reclaiming contaminated land reduces the threat to groundwater and enhances the rate of biodegradation.…”

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confidence: 99%

“…However, oily sludge is a complex mixture of alkane, aromatic, NSO (nitrogen-, sulfur-, and oxygen-containing compounds), and asphaltene fractions, and a single bacterial species has only limited capacity to degrade all the fractions of hydrocarbons present (4,5,9,20). Indigenous bacteria in the soil can degrade a wide range of target constituents of the oily sludge, but their population and efficiency are affected when any toxic contaminant is present at high concentrations (3,11,22). The reintroduction after enrichment of indigenous microorganisms isolated from a contaminated site helps to overcome this problem, as the microorganisms can degrade the constituents and have a higher tolerance to toxicity (14,17,21).…”

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confidence: 99%

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Evaluation of Inoculum Addition To Stimulate In Situ Bioremediation of Oily-Sludge-Contaminated Soil

Mishra

Jyot

Kuhad

et al. 2001

Appl Environ Microbiol

353

197

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A full-scale study evaluating an inoculum addition to stimulate in situ bioremediation of oily-sludgecontaminated soil was conducted at an oil refinery where the indigenous population of hydrocarbon-degrading bacteria in the soil was very low (10 3 to 10 4 CFU/g of soil). A feasibility study was conducted prior to the full-scale bioremediation study. In this feasibility study, out of six treatments, the application of a bacterial consortium and nutrients resulted in maximum biodegradation of total petroleum hydrocarbon (TPH) in 120 days. Therefore, this treatment was selected for the full-scale study. In the full-scale study, plots A and B were treated with a bacterial consortium and nutrients, which resulted in 92.0 and 89.7% removal of TPH, respectively, in 1 year, compared to 14.0% removal of TPH in the control plot C. In plot A, the alkane fraction of TPH was reduced by 94.2%, the aromatic fraction of TPH was reduced by 91.9%, and NSO (nitrogen-, sulfur-, and oxygen-containing compound) and asphaltene fractions of TPH were reduced by 85.2% in 1 year. Similarly, in plot B the degradation of alkane, aromatic, and NSO plus asphaltene fractions of TPH was 95.1, 94.8, and 63.5%, respectively, in 345 days. However, in plot C, removal of alkane (17.3%), aromatic (12.9%), and NSO plus asphaltene (5.8%) fractions was much less. The population of introduced Acinetobacter baumannii strains in plots A and B was stable even after 1 year. Physical and chemical properties of the soil at the bioremediation site improved significantly in 1 year.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Evaluation of Inoculum Addition To Stimulate In Situ Bioremediation of Oily-Sludge-Contaminated Soil

Mishra

Jyot

Kuhad

et al. 2001

Appl Environ Microbiol

353

197

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“…Lack of control over the parameters affecting microbial activity (temperature, pH, moisture, aeration, mixing, and circulation) prolongs treatment time (62,177,267,269,366,389,405,406,407,432). Maximum contaminant degradation occurs in the tilled surface, typically amounting to 10 to 20 cm of depth, although deeper aeration and mixing with ploughing and rotovating equipment has also been effectively implemented.…”

Section: Treatment Of Contaminated Soils and Sludgesmentioning

confidence: 99%

Recent Advances in Petroleum Microbiology

Hamme¹,

Singh

Ward

2003

Microbiol Mol Biol Rev

1,193

640

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Recent advances in molecular biology have extended our understanding of the metabolic processes related to microbial transformation of petroleum hydrocarbons. The physiological responses of microorganisms to the presence of hydrocarbons, including cell surface alterations and adaptive mechanisms for uptake and efflux of these substrates, have been characterized. New molecular techniques have enhanced our ability to investigate the dynamics of microbial communities in petroleum-impacted ecosystems. By establishing conditions which maximize rates and extents of microbial growth, hydrocarbon access, and transformation, highly accelerated and bioreactor-based petroleum waste degradation processes have been implemented. Biofilters capable of removing and biodegrading volatile petroleum contaminants in air streams with short substrate-microbe contact times (<60 s) are being used effectively. Microbes are being injected into partially spent petroleum reservoirs to enhance oil recovery. However, these microbial processes have not exhibited consistent and effective performance, primarily because of our inability to control conditions in the subsurface environment. Microbes may be exploited to break stable oilfield emulsions to produce pipeline quality oil. There is interest in replacing physical oil desulfurization processes with biodesulfurization methods through promotion of selective sulfur removal without degradation of associated carbon moieties. However, since microbes require an environment containing some water, a two-phase oil-water system must be established to optimize contact between the microbes and the hydrocarbon, and such an emulsion is not easily created with viscous crude oil. This challenge may be circumvented by application of the technology to more refined gasoline and diesel substrates, where aqueous-hydrocarbon emulsions are more easily generated. Molecular approaches are being used to broaden the substrate specificity and increase the rates and extents of desulfurization. Bacterial processes are being commercialized for removal of H2S and sulfoxides from petrochemical waste streams. Microbes also have potential for use in removal of nitrogen from crude oil leading to reduced nitric oxide emissions provided that technical problems similar to those experienced in biodesulfurization can be solved. Enzymes are being exploited to produce added-value products from petroleum substrates, and bacterial biosensors are being used to analyze petroleum-contaminated environments

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“…Microbial remediation of a hydrocarbon-contaminated site is accomplished with the help of a diverse group of microorganisms, particularly the indigenous bacteria present in the soil. These microorganisms can degrade a wide range of target constituents present in oily sludge (3,19). Pseudomonas spp., a group of gram-negative motile rods, have a remarkable ability to degrade a wide range of organic pollutants, including polycyclic aromatic hydrocarbons, halogenated derivatives, and recalcitrant organic residues (12).…”

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confidence: 99%

Evaluation of Genetic Diversity among Pseudomonas citronellolis Strains Isolated from Oily Sludge-Contaminated Sites

Bhattacharya

Sarma

Krishnan

et al. 2003

Appl Environ Microbiol

116

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The diversity among a set of bacterial strains that have the capacity to degrade total petroleum hydrocarbons (TPH) in soil contaminated with oily sludge (hazardous hydrocarbon waste from oil refineries) was determined. TPH is composed of alkane, aromatics, nitrogen-, sulfur-, and oxygen-containing compound, and asphaltene fractions of crude oil. The 150 bacterial isolates which could degrade TPH were isolated from soil samples obtained from diverse geoclimatic regions of India. All the isolates were biochemically characterized and identified with a Biolog microbial identification system and by 16S rDNA sequencing. Pseudomonas citronellolis predominated among the 150 isolates obtained from six different geographically diverse samplings. Of the isolates, 29 strains of P. citronellolis were selected for evaluating their genetic diversity. This was performed by molecular typing with repetitive sequence (Rep)-based PCR with primer sets ERIC (enterobacterial repetitive intergenic consensus), REP (repetitive extragenic palindromes), and BOXAIR and PCR-based ribotyping. Strain-specific and unique genotypic fingerprints were distinguished by these molecular typing strategies. The 29 strains of P. citronellolis were separated into 12 distinguishable genotypic groups by Rep-PCR and into seven genomic patterns by PCR-based ribotyping. The genetic diversity of the strains was related to the different geoclimatic isolation sites, type of oily sludge, and age of contamination of the sites. These results indicate that a combination of Rep-PCR fingerprinting and PCR-based ribotyping can be used as a highresolution genomic fingerprinting method for elucidating intraspecies diversity among strains of P. citronellolis.

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Aerobic degradation of a hydrocarbon mixture in natural uncontaminated potting soil by indigenous microorganisms at 20 °C and 6 °C

Cited by 46 publications

References 14 publications

Evaluation of Inoculum Addition To Stimulate In Situ Bioremediation of Oily-Sludge-Contaminated Soil

Evaluation of Inoculum Addition To Stimulate In Situ Bioremediation of Oily-Sludge-Contaminated Soil

Recent Advances in Petroleum Microbiology

Evaluation of Genetic Diversity among Pseudomonas citronellolis Strains Isolated from Oily Sludge-Contaminated Sites

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