Aerobic cervical bacteriology and antibiotic sensitivity patterns in patients with advanced cervical cancer before and after radiotherapy at a national referral hospital in Uganda

Mubangizi, Louis; Namusoke, Fatuma; Mutyaba, Twaha

doi:10.1016/j.ijgo.2014.01.013

Cited by 7 publications

(13 citation statements)

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“…The yield of anaerobic bacteria was very poor, even though specific measures like the use of enrichment media for transportation of cervicovaginal samples were employed in this study. Several studies from high income settings have strongly associated them with PROM [ 4 ], so it would be interesting to study the whole picture in a better resourced laboratory before drawing conclusions [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…Each participant was requested to give written informed consent. An interviewer administered questionnaire was used to collect data on patient demographics and clinical features [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

“…Specimen was transported in 3 separate containers: 1 mL inoculated into a selective Todd-Hewitt enrichment media to culture for GBS in a glass bottle; 1 mL inoculated into thioglycolate enrichment media to culture for facultative anaerobes in a glass bottle; 3 mL in a sterile plastic container. The aerobic culture was performed on blood, chocolate, and MacConkey agar incubated at 35–37° for 24–48 hours [ 12 ]. Colonial morphology, gram staining, and biochemical analysis were used to identify the isolates.…”

Section: Methodsmentioning

confidence: 99%

“…The Kirby-Bauer disk diffusion and dilution methods were used to test for sensitivity of the isolates against nitroimidazoles, penicillins, cephalosporins, sulfonamides, aminoglycosides, quinolones/fluoroquinolones, macrolides, and tetracyclines, as specified in the manual of clinical microbiology (Jorgensen, Pfaller et al 2015). Ten percent of the samples were taken to 2 laboratories (Medical School Laboratory and MBN diagnostic laboratories) for quality control purposes [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

“…Data was entered into Epidata version 3.1 and then exported into Stata version 12 for analysis. The proportion of positive amniotic fluid cultures was computed; statistical significance was tested via the chi ( χ 2 ) test [ 12 ]. The paired t -test was applied to measure the significance of the factors associated with positive cultures ( P = 0.05).…”

Section: Methodsmentioning

confidence: 99%

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Cervicovaginal Bacteriology and Antibiotic Sensitivity Patterns among Women with Premature Rupture of Membranes in Mulago Hospital, Kampala, Uganda: A Cross-Sectional Study

Musaba

Kagawa

Kiggundu

et al. 2017

Infectious Diseases in Obstetrics and Gynecology

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Background. A 2013 Cochrane review concluded that the choice of antibiotics for prophylaxis in PROM is not clear. In Uganda, a combination of oral erythromycin and amoxicillin is the 1st line for prophylaxis against ascending infection. Our aim was to establish the current cervicovaginal bacteriology and antibiotic sensitivity patterns. Methods. Liquor was collected aseptically from the endocervical canal and pool in the posterior fornix of the vagina using a pipette. Aerobic cultures were performed on blood, chocolate, and MacConkey agar and incubated at 35–37°C for 24–48 hrs. Enrichment media were utilized to culture for GBS and facultative anaerobes. Isolates were identified using colonial morphology, gram staining, and biochemical analysis. Sensitivity testing was performed via Kirby-Bauer disk diffusion and dilution method. Pearson's chi-squared (χ2) test and the paired t-test were applied, at a P value of 0.05. Results. Thirty percent of the cultures were positive and over 90% were aerobic microorganisms. Resistance to erythromycin, ampicillin, cotrimoxazole, and ceftriaxone was 44%, 95%, 96%, and 24%, respectively. Rupture of membranes (>12 hrs), late preterm, and term PROM were associated with more positive cultures. Conclusion. The spectrum of bacteria associated with PROM has not changed, but resistance to erythromycin and ampicillin has increased.

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Section: Discussionmentioning

confidence: 99%

“…Each participant was requested to give written informed consent. An interviewer administered questionnaire was used to collect data on patient demographics and clinical features [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Cervicovaginal Bacteriology and Antibiotic Sensitivity Patterns among Women with Premature Rupture of Membranes in Mulago Hospital, Kampala, Uganda: A Cross-Sectional Study

Musaba

Kagawa

Kiggundu

et al. 2017

Infectious Diseases in Obstetrics and Gynecology

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Recycling the Interspecific Relations with Epithelial Cells: Bacteria and Cancer Metabolic Symbiosis

Nunes

Serpa

2020

Advances in Experimental Medicine and Biology

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Cervical cancer prevention and treatment research in Africa: a systematic review from a public health perspective

et al. 2016

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BackgroundWomen living in Africa experience the highest burden of cervical cancer. Research and investment to improve vaccination, screening, and treatment efforts are critically needed. We systematically reviewed and characterized recent research within a broader public health framework to organize and assess the range of cervical cancer research in Africa.MethodsWe searched online databases and the Internet for published articles and cervical cancer reports in African countries. Inclusion criteria included publication between 2004 and 2014, cervical cancer-related content pertinent to one of the four public health categories (primary, secondary, tertiary prevention or quality of life), and conducted in or specifically relevant to countries or regions within the African continent. The study design, geographic region/country, focus of research, and key findings were documented for each eligible article and summarized to illustrate the weight and research coverage in each area. Publications with more than one focus (e.g. secondary and tertiary prevention) were categorized by the primary emphasis of the paper. Research specific to HIV-infected women or focused on feasibility issues was delineated within each of the four public health categories.ResultsA total of 380 research articles/reports were included. The majority (54.6 %) of cervical cancer research in Africa focused on secondary prevention (i.e., screening). The number of publication focusing on primary prevention (23.4 %), particularly HPV vaccination, increased significantly in the past decade. Research regarding the treatment of precancerous lesions and invasive cervical cancer is emerging (17.6 %), but infrastructure and feasibility challenges in many countries have impeded efforts to provide and evaluate treatment. Studies assessing aspects of quality of life among women living with cervical cancer are severely limited (4.1 %). Across all categories, 11.3 % of publications focused on cervical cancer among HIV-infected women, while 17.1 % focused on aspects of feasibility for cervical cancer control efforts.ConclusionsCervical cancer research in African countries has increased steadily over the past decade, but more is needed. Tertiary prevention (i.e. treatment of disease with effective medicine) and quality of life of cervical cancer survivors are two severely under-researched areas. Similarly, there are several countries in Africa with little to no research ever conducted on cervical cancer.

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Aerobic cervical bacteriology and antibiotic sensitivity patterns in patients with advanced cervical cancer before and after radiotherapy at a national referral hospital in Uganda

Abstract: Four weeks of EBRT did not sterilize the cervix but resulted in an increase in normal flora. Radiotherapy appeared to reduce resistance to commonly used antibiotics. Sensitivity to chloramphenicol was higher than for the more commonly used antibiotics.

Cited by 7 publications

References 10 publications

Cervicovaginal Bacteriology and Antibiotic Sensitivity Patterns among Women with Premature Rupture of Membranes in Mulago Hospital, Kampala, Uganda: A Cross-Sectional Study

Cervicovaginal Bacteriology and Antibiotic Sensitivity Patterns among Women with Premature Rupture of Membranes in Mulago Hospital, Kampala, Uganda: A Cross-Sectional Study

Recycling the Interspecific Relations with Epithelial Cells: Bacteria and Cancer Metabolic Symbiosis

Cervical cancer prevention and treatment research in Africa: a systematic review from a public health perspective

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