Aerobic biodegradation of N‐nitrosodimethylamine (NDMA) by axenic bacterial strains

Sharp, Jonathan O.; Wood, Thomas K.; Alvarez‐Cohen, Lisa

doi:10.1002/bit.20405

Cited by 104 publications

(107 citation statements)

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“…ENVPC5 (McClay et al, 1995) are able to degrade NDMA (Table 1; Streger et al, 2003;Sharp et al, 2005). The involvement of a T4MO enzyme in NDMA degradation was confirmed using a P. putida strain transformed with a vector expressing T4MO (Figure 1; Streger et al, 2003;.…”

Section: Pathway Of Ndma Degradation By Pseudomonas Mendocina Kr1mentioning

confidence: 95%

“…Growth and assay conditions To evaluate degradation intermediates, ENV425 was grown in Tryptic Soy Broth (TSB; BD, Franklin Lakes, NJ), basal salts medium (BSM-H) (Hareland et al, 1975) with propane (~ 25% of headspace gas) or basal salts medium (BSM-S; Sharp et al, 2005) with glucose (20 mM) and NDMA (270 μM). The cells were incubated at 30°C under aerobic conditions and agitated on a rotary shaker at 175 rpm.…”

Section: Materials and Methods: Ndma Transformation By R Ruber Env425mentioning

confidence: 99%

“…The bacterium was initially cultured in a nephelo culture flask (Bellco Biotechnology) with basal salts medium (BSM-S; Sharp et al, 2005). Neat toluene (100 µL per 100 mL culture) was placed in the sidearm tube of the flask.…”

Section: Assay Conditionsmentioning

confidence: 99%

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Bioremediation Approaches for Treating Low Concentrations of N-Nitrosodimethylamine in Groundwater

Hatzinger¹,

Hawari²,

Fournier³

2008

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The public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing the burden, to the Department of Defense, Executive Services and Communications Directorate (0704-0188). Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number.

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Section: Pathway Of Ndma Degradation By Pseudomonas Mendocina Kr1mentioning

confidence: 95%

Section: Materials and Methods: Ndma Transformation By R Ruber Env425mentioning

confidence: 99%

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Bioremediation Approaches for Treating Low Concentrations of N-Nitrosodimethylamine in Groundwater

Hatzinger¹,

Hawari²,

Fournier³

2008

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“…Because NDMA can be mineralized in oxic systems by a monooxygenase enzyme pathway (Sharp et al 2005(Sharp et al , 2007, methane-, propane-, and toluene-monooxygenase pathways were investigated in oxic, anaerobic, and reduced sediment to investigate the relative contribution of this biotic mineralization (generally in oxic systems by the monooxygenase pathways) and potential abiotic contributions. In these experiments, quantities of methane or propane gas (or aqueous toluene) were added to the respective oxic, anaerobic, or reduced sediment system (contains headspace) at the same time the 14 C-labeled NDMA was added (i.e., no preconditioning time).…”

Section: Carbon Additions In Oxic and Reduced Systemsmentioning

confidence: 99%

Abiotic and Biotic Mechanisms Controlling In Situ Remediation of NDMA

Szecsody¹,

McKinley²,

Breshears³

et al. 2009

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Public reporting burden for the collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden, to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to a penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. REPORT DATE MAY 20092. REPORT iii Executive SummaryThis project was initiated to investigate whether in situ coupled abiotic/biotic degradation of N-nitrosodimethylamine (NDMA, an emerging contaminant) could be used as a permeable reactive barrier for remediation at the Aerojet, California site, where groundwater contains up to 36-ppb NDMA. The sediment mainly used in experiments is from the Aerojet groundwater aquifer (260-ft depth), with additional sediments from other groundwater aquifers (i.e., not shallow soils, Ft. Lewis, Washington, 60-ft depth, Puchack, New Jersey, 273-ft depth). Different in situ remediation processes were compared in this study: a) biostimulation (oxic, anaerobic, iron-reducing, sulfate-reducing), b) abiotic iron-reducing environment (by dithionite reduction of sediment or zero valent iron addition), c) coupled abiotic/biotic remediation (iron-reducing environment), and d) sequential iron-reducing, then oxic, biostimulation. The overall goal was to understand and optimize the combined effects of abiotic and biotic processes to degrade NDMA to nontoxic products. Iron-reducing conditions were created by chemical reduction of a sediment using sodium dithionite, and in a few cases, a natural reduced aquifer sediment or sediment with zero valent iron addition.When subsurface sediments are chemically or naturally reduced, abiotic surface phase(s) rapidly degraded NDMA (8-hour half-life for high reduction, slower for low reduction) to nontoxic dimethylamine (DMA). Experiments showed up to 80% conversion of NDMA to DMA, with further degradation to nitrate (up to 40% molar conversion), formate (trace concentration) and carbon dioxide (up to 82% molar conversion). Methylamine and formaldehyde (likely intermediates) were not detected. Experiments with 100 and 10 ng/L NDMA starting concentration had a rapid degradation half-life (4.7 hours), and NDMA was degraded to <3 ng/L (detection limit). Although degradation to DMA is sufficient for remediation (DMA is not toxic at <5 mg/L), because NDMA mass was degraded further to more toxic intermediates, NDMA mineralization (i.e., to CO 2 ) was considered the lowest risk product, and was the main focus of this study. NDMA degradation was most rap...

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“…Gunnsion et al [11] reported on the aerobic and anaerobic biodegradation of NDMA in from a contaminated groundwater site. Sharp et al [12], Kaplan and Kaplan [13] and Fournier et al [14] have reported on biodegradation pathways of NDMA using pure bacterial strains. All these studies indicate that NDMA biodegradation is feasible with higher concentrations exhibiting inhibition to bacterial cultures.…”

Section: Introductionmentioning

confidence: 99%

Fate of Nitrosodimethylamine (NDMA)

Jahan¹,

Smith²,

Scrivani³

et al. 2008

WIT Transactions on Ecology and the Environment

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Nitrosodimethylamine (NDMA) is a known animal carcinogen and is noted as a probable human carcinogen by the USEPA with a lifetime risk of contracting cancer of 10 -6 at 0.7 ng/L. This contaminant is of rising concern since its production is linked to disinfection processes at water and wastewater treatment plants. The formation of NDMA is also associated with the production of rocket fuel. Therefore, a need to develop an effective treatment process to remove NDMA from water is needed. Batch experiments were conducted to determine the biodegradability and photodegradation of NDMA at varying concentrations. Biodegradation experiments were conducted with a mixed bacterial culture obtained from a wastewater treatment plant. Oxygen uptake and cell growth was monitored with time. Results indicated that NDMA was biodegradable with some inhibition at higher concentrations. Batch photodegradation experiments were carried out in the presence and absence of titanium dioxide (TiO 2 ) a common catalyst used to accelerate photodegradation. The removal of NDMA using photodegradation appears to be a feasible method. Initial removal rates are high. Lower pH and the addition of titanium dioxide accelerated the removal of NDMA. During the degradation of NDMA, by-products such as dimethylamine (DMA), formaldehyde, and nitrate were produced and remained at nearly constant concentrations.

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Aerobic biodegradation of N‐nitrosodimethylamine (NDMA) by axenic bacterial strains

Cited by 104 publications

References 58 publications

Bioremediation Approaches for Treating Low Concentrations of N-Nitrosodimethylamine in Groundwater

Bioremediation Approaches for Treating Low Concentrations of N-Nitrosodimethylamine in Groundwater

Abiotic and Biotic Mechanisms Controlling In Situ Remediation of NDMA

Fate of Nitrosodimethylamine (NDMA)

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