Aerobic biodegradation of microcystin-LR by an indigenous bacterial mixed culture isolated in Taiwan

Tsao, Sha; Wei, Da-Jiun; Chang, Yin‐Cheng; Lee, Jiunn‐Fwu

doi:10.1016/j.ibiod.2017.04.011

Cited by 21 publications

(18 citation statements)

References 30 publications

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“…However, the range of half-lives observed in all consortia including the present study are longer than those of MC-degrading bacterial isolates reported previously ( Figure S18). In comparison with previous study designs that were focused on MC degrading microbial consortia [23,34,61], this study is significantly different in which we have isolated the MC-degrading consortia by sub-culturing in minimal media enriched with MC. We have demonstrated the stability and unique individual characteristics of each isolated consortia for MC-degradation.…”

Section: Discussionmentioning

confidence: 96%

“…Other (fewer) studies have demonstrated that the addition of an alternative organic carbon source facilitates MC degradation rates, where the organic carbon was postulated to stimulate the growth and energy availability for production of the necessary enzymes required for MC-degradation by these bacterial populations [31,32] In addition, several studies have reported a net neutral effect with the addition of alternative organic carbon sources [7,8,26,27]. The community structure of the microbial consortia that was involved in MC biodegradation was shown to change with environmental conditions [8,27,33,34]. For example, Mou and co-workers [33] reported that Methylophilales (Methylotenera genus) and Burkholderiales (Bortadella, Burkholderia, Cupriavidus, Ralstonia genera) were the most significant taxa in microcosms containing MC.…”

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confidence: 99%

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The Effect of Organic Carbon Addition on the Community Structure and Kinetics of Microcystin-Degrading Bacterial Consortia

Manheim

Cheung

Jiang

2018

Water

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Microcystin (MC), a hepatotoxin that is associated with cyanobacterial blooms in freshwater lakes, threatens the quality of drinking water resources. Biodegradation of MC using biofiltration is emerging as a cost-effective solution for drinking water treatment. This study reports isolation of five MC-degrading microbial consortia and investigation of their community structure and kinetics in the presence or absence of a readily-bioavailable organic carbon source. The results indicated that the presence of a bioavailable organic carbon source caused: (1) the proliferation of community members previously unobserved in each consortium cultured without ethanol; (2) a shift in abundance of representative taxa; (3) a fluctuation in genera affiliated with MC-biodegradation; and, (4) a unique response in simulated diversity among consortia. These changes to each microbial consortium were paralleled by a significant decline in MC degradation kinetics. Overall, this study highlights the importance of integrating environmental conditions into the design and operation of biofiltration systems for MC biodegradation.Previous studies have demonstrated the complete biodegradation of MC-LR within laboratory scale biofiltration systems following a preliminary lag phase of removal [14][15][16][17]. However, the toxin removal efficiency and extent of this lag phase can vary significantly with bacterial composition, nutrient concentration, and other environmental parameters that are associated with the source water. Efforts to evolve biofiltration from a passive process into a more standardized, controlled, and perhaps "engineered", biological treatment process for targeted removal of pollutants will require a better understanding of the physiology and genetics of MC-degrading bacteria [12][13][14][18][19][20].Significant efforts have been made to isolate and characterize the specific MC-degrading bacterial populations during algal bloom events in the source water and sediments, and from full scale biological treatment units in drinking water treatment facilities [7][8][9]17,[21][22][23][24]. However, these studies have not fully explored how these isolates function in mixed bacterial communities, nor the influences of mutualistic or antagonistic interactions on biodegradation kinetics. Several previous studies, however, have considered the effects of environmental stimuli (i.e., varying organic carbon concentrations) on MC-degrading bacterial consortia under aerobic conditions [7,8,23,[25][26][27][28][29]. Research showed that addition of organic carbon sources (i.e., glucose, acetate, or uncharacterized dissolved organic carbon (DOC)) significantly inhibits MC degradation kinetics of bacterial consortia [7,23,25,29]. Catabolite repression is postulated as a mechanism underlying this inhibition, where MC-degrading populations may prefer more energetically-favorable (easily metabolized) over more energetically-intensive substrates, such as MC [30]. Other (fewer) studies have demonstrated that the addition of an alternative organic ca...

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Section: Discussionmentioning

confidence: 96%

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confidence: 99%

The Effect of Organic Carbon Addition on the Community Structure and Kinetics of Microcystin-Degrading Bacterial Consortia

Manheim

Cheung

Jiang

2018

Water

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“…Microbial biodegradation is an environmentally-friendly and effective treatment method for detoxify MC-LR in natural waters without potential harmful by-products. Some MC-degrading pure bacterial strains have been isolated and have their MC-LR-degrading rates reported [6, 16, 17, 19]. For example, the single pure bacterial strain Sphingomonas sp.…”

Section: Discussionmentioning

confidence: 99%

“…DC7 and Microbacterium sp.DC8 degraded MC at 0.18 µg/(ml·d) while individual DC7 or DC8 can’t degrade MC-LR respectively. Tsao et al [19] discovered a mixed culture with MC-degradation rate of 0.876 µg/(ml·d), which contained Sphingomonas sp., Pseudoxanthomonas sp., Hyphomicrobium aestuarii , Sphingobium sp., Rhizobium sp., Steroidobacter sp. and Acinetobacter sp.…”

Section: Discussionmentioning

confidence: 99%

“…A few MC-degrading pure bacterial strains have been isolated, identified and had their mechanisms reported, and most of the isolated MC-degrading bacteria were limited to the family Sphingomonadaceae [16-18]. In practice, native bacterial communities (indigenous bacterial mixed culture) may be more suitable for degrading MC-LR in the environment compared to the single pure bacterial strains [6,19]. Therefore, it’s very interesting and important to obtain some native mixed bacterial communities for MC-LR removal.…”

Section: Introductionmentioning

confidence: 99%

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Aerobic removal of microcystin-LR by a novel native effective bacterial community designated as YFMCD4 isolated from Lake Taihu

Guo

Huang

Massey

et al. 2018

Preprint

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12 Microcystins (MCs) are a group of monocyclic heptapeptide hepatotoxins produced by species of 13 cyanobacteria. MC-LR is the most toxic and frequently detected MCs variant in water, which 14 poses a great threat to the natural ecosystem and public health. It's important to seek 15 environment-friendly and cost-efficient methods to remove MC-LR. To investigate the MC-16 degrading capacities of a novel indigenous bacterial community designated as YFMCD4 and the 17 influence of environmental factors including various temperatures, MC concentrations and pH on 18 the MC-degrading activities, the concentration of MC-LR was measured by high performance 19liquid chromatography. In addition, the MC-degrading mechanism containing the degradation 20 pathway and products of YFMCD4 was studied using HPLC coupled with an ultra-high 21 resolution LTQ Orbitrap Velos Pro ETD mass spectrometry equipped with electrospray 22 ionization interface. The data showed MC-LR can be removed at the maximum rate of 0.5 23 µg/(ml·h) by YFMCD4 containing Alcaligenes faecalis and Stenotrophomonas acidaminiohila. 24The MC-degrading rates of YFMCD4 were significantly affected by different temperatures, pH 25 and MC-LR concentrations. Two intermediates of a tetrapeptide and Adda appeared in the 26 degradation process. These results illustrate that the novel bacterial community YFMCD4 can 27 remove MC-LR effectively and completely, which indicates YFMCD4 possesses a significant 28 potential to be used in bioremediation of water bodies contaminated by MC-LR. 29 3 47 MC-LR is very stable and resistant to many natural factors including extreme pH, high 48 temperature and sunlight in the environment owing to the cyclic structure [3, 6, 12]. Moreover, 49 MC-LR can be accumulated in aquatic organisms and food crops representing a health hazard to 50 human and animals through food chains [13]. It is very important to reduce MC-LR 51 concentration in freshwater ecosystem. However, conventional drinking water treatments have 52 limited efficacy in removing MC-LR. Some physical and chemical methods containing 53 ozonation, chlorination, photocatalysis and electrolysis have been proposed for MC-LR 54 elimination from drinking water. However, all these methods have certain limitations in terms of 55 high operating costs, low efficacy and harmful by-products [3, 6, 14, 15]. It's desirable that 56investigators seek other environmentally-benign and cost-efficient methods and technologies to 57 remove MC-LR found in water bodies [3, 6,[14][15][16][17]. 58Several previous investigations demonstrated that microbial biodegradation may be one of 59 the most environmentally-friendly, effective and promising treatment methods for removing 60 MC-LR in natural waters, since it can detoxify MC-LR and don't generated any apparent 61 potential harmful by-products [3, 6,[14][15][16][17]. A few MC-degrading pure bacterial strains have 62 been isolated, identified and had their mechanisms reported, and most of the isolated MC-63 degrading bacteria were limited to the family S...

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Microcystin-LR Degradation Following Copper-Based Algaecide Exposures

Kinley

Iwinski-Wood²,

Geer

et al. 2018

Water Air Soil Pollut

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Aerobic biodegradation of microcystin-LR by an indigenous bacterial mixed culture isolated in Taiwan

Cited by 21 publications

References 30 publications

The Effect of Organic Carbon Addition on the Community Structure and Kinetics of Microcystin-Degrading Bacterial Consortia

The Effect of Organic Carbon Addition on the Community Structure and Kinetics of Microcystin-Degrading Bacterial Consortia

Aerobic removal of microcystin-LR by a novel native effective bacterial community designated as YFMCD4 isolated from Lake Taihu

Microcystin-LR Degradation Following Copper-Based Algaecide Exposures

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