Green
and dark tea extract (GTE/DTE) ameliorate chemical-induced
colitis in mice; however, the role of gut microbiota in the anticolitis
effects of green and dark tea in mice remains unclear. This study
aims to explore the role of modulations in gut microbes mediated by
green and dark tea in colitis mice by fecal microbiota transplantation
(FMT). Our results indicated that GTE and DTE (5 mg/kg bodyweight/day
for 4 weeks) exhibited prebiotic effects on the donor mice. Moreover,
the FMT treatments (transferring the microbiota daily from the 1 g/kg
bodyweight fecal sample to each recipient) indicated that, compared
with the fecal microbiota from the normal diet-treated donor mice,
the fecal microbiota from the GTE- and DTE-treated donor mice significantly
ameliorate colitis-related symptoms (e.g., loss of bodyweight, colonic
inflammation, loss of barrier integrity, and gut microbiota dysbiosis)
and downregulated the TLR4/MyD88/NF-κB pathway. Collectively,
GTE and DTE ameliorate chemical-induced colitis by modulating gut
microbiota.