Aerobic and anaerobic swimming speeds of spermatozoa investigated by twin beam laser velocimetry

Wilson, Mariangela C.; Harvey, J. K.; Shannon, Patrick

doi:10.1016/s0006-3495(87)83373-8

Cited by 16 publications

(7 citation statements)

References 7 publications

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“…Increased O 2 uptake was described for rams during testicular insulation, but there was no apparent difference in lactate concentrations, and therefore, no indication that sperm switched from aerobic to anaerobic metabolism (Waites and Setchell 1964). It is noteworthy that anaerobic metabolism dramatically reduced sperm motility in both bulls and rams (Wilson et al 1987). In human mountain climbers, chronic hypoxia reduced sperm motility and concentration and increased the number of immature sperm in the ejaculate (Verratti et al 2008).…”

Section: Discussionmentioning

confidence: 95%

Hyperthermia and not hypoxia may reduce sperm motility and morphology following testicular hyperthermia

Kastelic¹,

Wilde²,

Rizzoto³

et al. 2017

Vet. Med.

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ABSTRACT:The mammalian testis typically operates on the brink of hypoxia; the long-standing dogma is that increased testicular temperature increases metabolism, but blood flow is unaffected and the resulting hypoxia reduces sperm motility and morphology. In rats and mice, oxygen (O 2 ) content of inspired air affected O 2 content of testes, enabling the latter to range from approximately 50 to more than 200% of physiologic concentrations. A ram model was used to test the hypotheses that hypoxia would disrupt sperm motility and morphology and that hyperoxia would prevent hyperthermia-induced reductions in sperm motility and morphology. Eighteen Canadian Arcott rams (approximately 10 months old) were used in a 2 × 3 factorial, with factors being scrotal insulation (insulated or not insulated) and O 2 concentrations in inspired air (14, 21 or 85%). Six rams, three with and three without scrotal insulation, were placed in each of three enclosed areas for 30 h to expose them to their respective oxygen concentrations, with scrotal insulation removed at the end of the exposure. Semen was collected by electro-ejaculation twice before insulation, bi-weekly for four weeks starting one week after exposures, and then once weekly for two weeks. There were effects of insulation, time and an insulation × time interaction for motile sperm and sperm that had normal morphology or head or midpiece defects (P < 0.01 for each). Sperm motility and morphology exhibited alterations between approximately two and five weeks after insulation, with mean progressively motile and morphologically normal sperm decreasing from approximately 55 to 35% and from 80 to 30%, respectively, and with head and midpiece defects increasing from approximately 3 to 50% and from 10 to 20% (P < 0.05 for each). The hypotheses that hypoxia would disrupt sperm quality and production, whereas hyperoxia would prevent hyperthermia-induced reductions in sperm quality and production, were not supported. This is apparently the first report that heat-stress induced damage to sperm was due to increased temperature per se and not testicular hypoxia, calling into question a long-standing paradigm.

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Section: Discussionmentioning

confidence: 95%

Hyperthermia and not hypoxia may reduce sperm motility and morphology following testicular hyperthermia

Kastelic¹,

Wilde²,

Rizzoto³

et al. 2017

Vet. Med.

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“…The parameters determined by LDV are the number and proportion of motile spermatozoa, the velocity distribution and the three-dimensional instantaneous ('characteristic') velocity (Dubois et al, 1975;Naylor et al, 1982;Craig et al, 1982;Woolford and Harvey, 1982;Rigler and Thyberg, 1984;Pusch, 1985;Earnshaw et al, 1985). Variations of this technique which have been used in the evaluation of sperm motility are Photon Correlation Spectroscopy, which is a fully digital technique (Frost and Cummins, 1981) and twin-beam laser velocimetry (Wilson and Harvey, 1983;Wilson et al, 1987). The advantage of the latter method is that the translational and rotational velocity values are not combined.…”

Section: Laser Doppler Velocimetry (Ldv)mentioning

confidence: 99%

A critical review of the physiological importance and analysis of sperm movement in mammals

Mortimer

1997

Human Reproduction Update

292

201

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The identification of human sperm hyperactivated motility has potential importance in sperm function tests, as well as in quality control assays and in reproductive toxicology investigations. However, relatively little is known about this phenomenon and the variety of definitions used for hyperactivation has led to a great deal of confusion as to its occurrence and physiological relevance. This presentation is a critical review of a number of aspects of hyperactivated motility, including its identification and potential role(s) in mammalian fertilization. The initial sections of the review consider the mechanisms involved in the development and maintenance of mammalian sperm motility, and the structural and functional changes in spermatozoa which occur during transport through the female reproductive tract. The methods available for the quantification of aspects of sperm movement are also discussed, with an historical overview of sperm movement analysis.

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“…We explored the validity of this relationship by monitoring motility over 120 min in three independent, thawed undiluted samples (see caption of Fig 8 for details). These parameters changed with time, especially as the cells gradually depleted the suspending medium of oxygen [45, 46]. The three samples monitored over time therefore gave a range of these parameters: , A 0 = 2-4 μm and f 0 = 5-20 Hz.…”

Section: Resultsmentioning

confidence: 99%

High-throughput characterisation of bull semen motility using differential dynamic microscopy

et al. 2019

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We report a high-throughput technique for characterising the motility of spermatozoa using differential dynamic microscopy. A movie with large field of view (∼10mm 2 ) records thousands of cells (e.g. ≈ 5000 cells even at a low cell density of 20 × 10 6 cells/ml) at once and yields averaged measurements of the mean ( ) and standard deviation ( σ ) of the swimming speed, head oscillation amplitude ( A 0 ) and frequency ( f 0 ), and the fraction of motile spermatozoa ( α ). Interestingly, we found that the measurement of α is facilitated because the swimming spermatozoa enhance the motion of the non-swimming population. We demonstrate the ease and rapidity of our method by performing on-farm characterisation of bull spermatozoa motility, and validate the technique by comparing laboratory measurements with tracking. Our results confirm the long-standing theoretical prediction that for swimming spermatozoa.

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Aerobic and anaerobic swimming speeds of spermatozoa investigated by twin beam laser velocimetry

Cited by 16 publications

References 7 publications

Hyperthermia and not hypoxia may reduce sperm motility and morphology following testicular hyperthermia

Hyperthermia and not hypoxia may reduce sperm motility and morphology following testicular hyperthermia

A critical review of the physiological importance and analysis of sperm movement in mammals

High-throughput characterisation of bull semen motility using differential dynamic microscopy

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