Aerobactin Synthesis Genes iucA and iucC Contribute to the Pathogenicity of Avian Pathogenic Escherichia coli O2 Strain E058

Ling, Jielu; Pan, Hongyu; Gao, Qingqing; Xiong, Liping; Zhou, Yefei; Zhang, Debao; Gao, Song; Liu, Xiufan

doi:10.1371/journal.pone.0057794

Cited by 46 publications

(40 citation statements)

References 43 publications

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“…Tivendale et al (2004) demonstrated the ability of the three most virulent APEC strains to amplify the iucA gene in comparison with less virulent APEC strains. Other studies have also suggested that iucA plays an important role in the pathogenicity of APEC (Ling et al, 2013;Xiong et al, 2012).…”

Section: Discussionmentioning

confidence: 92%

“…Aerobactin is synthesised by the iucABCD encoded gene (iron uptake chelate) and is taken up by the IutA-encoded receptor protein (Bindereif and Neilands, 1985;Ling et al, 2013). When compared to AFEC, aerobactin biosynthetic genes are more frequently detected in APEC and their incidence correlates with highly pathogenic strains (Dozois et al, 1992;Lafont et al, 1987;Linggood et al, 1987).…”

Section: Aerobactinmentioning

confidence: 99%

“…Originally, the iutA gene was found to be encoded on the pColV-K30 plasmid (Warner et al, 1981;Williams and Warner, 1980) and later it was detected in the chromosome (Crosa, 1989). A study in China investigated the contributions of the iutA gene to APEC pathogenicity mechanisms by deleting the iutA gene (Ling et al, 2013). This study reported a significant decrease in APEC pathogenicity of the mutant strains, w hich indicates that the iutA gene plays an important role in pathogenicity (Ling et al, 2013).…”

Section: Aerobactinmentioning

confidence: 99%

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Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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Avian pathogenic Escherichia coli (APEC) is the causative agent of avian colibacillosis, a localised or systemic infection resulting in clinical diseases such as colisepticemia, chronic respiratory disease and swollen-head syndrome. Globally, avian colibacillosis is the leading cause of morbidity and mortality in poultry, and it has been associated with massive economic losses and welfare problems.This organism is of public health significance as APEC is communicable to humans. The diagnosis of avian colibacillosis relies on clinical signs, typical pathological lesions and culture of E. coli from affected tissue(s). Antimicrobial therapy is often used both for treatment and control. Previous overseas studies have characterised APEC and identified virulence genes (VGs) that can be used as molecular markers for the identification of APEC. Little is known about APEC in broiler chickens in Australia.The aim of this thesis was to gain a better understanding of the epidemiology of APEC in Australian broiler flocks and how factors including presence of VGs and phylogenetic group can improve the identification of this pathotype in Australia. Firstly, three faecal DNA extraction methods were evaluated. Faeces were collected from healthy chickens and chickens with colibacillosis from commercial broiler farms in South East Queensland (SEQ). The extracted DNA was screened by a pentaplex-PCR for five APEC-associated VGs (iroN, iutA, iss, hlyF and ompT). DNA extracted from E. coli isolates cultured from the cloaca and organs of the birds were screened using the same PCR.Repeated bead beating plus column elution was the preferred DNA extraction method, as it yielded good PCR quality and adequate quantity DNA. However, identifying APEC by direct detection of the five VGs from the faecal material was not feasible as all of these genes were also detected in all of the birds. However, the VGs were more commonly detected in E. coli isolates cultured from birds with colibacillosis.A cross-sectional study was performed to estimate APEC farm-level prevalence in healthy broiler chickens in SEQ and to identify potential risk factors associated with the carriage of APEC. At the farm-level, all of the 40 farms sampled were positive for APEC, that is at least one bird per farm carried APEC, while the within-farm prevalence was 63% (95% Confidence Interval: 55.8, 70.2).Higher APEC within-farm bird-level prevalence was significantly associated with the usage of well water as a source of drinking water, failure to disinfect the water line after each flock, farm visitors not showering before entering the shed, distances greater than 20 metres between the car park and the poultry shed and the presence of wild birds within 50 metres of the shed. Chlorinating the drinking water combined with automatic water filtration reduced within-farm bird-level APEC prevalence. Page | 3Therefore, based on the results concluded from the multivariable model, improving biosecurity and water treatments might reduce APEC prevalence, decrease the risk of co...

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Section: Discussionmentioning

confidence: 92%

Section: Aerobactinmentioning

confidence: 99%

Section: Aerobactinmentioning

confidence: 99%

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Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Awawdeh¹,

Turni²,

Henning³

et al.

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“…The salmochelin iron uptake system, encoded on the chromosome of Salmonella species and of several uropathogenic E. coli (UPEC) (95-98), has been recently associated with the ColV plasmids of APEC strains (90,92,99,100) as well as with non-ColV transmissible plasmids of UPEC and Klebsiella strains (101,102).…”

Section: Salmochelin Iron Uptake Systemmentioning

confidence: 99%

“…Among avian E. coli isolates, only the most virulent harbor the aerobactin genes, while these same genes are absent from nonvirulent and nonlethal isolates (88,89). Furthermore, aerobactin genes were expressed during infection, and defined deletion mutants in biosynthetic and transport genes resulted in decreased virulence in a chicken infection model (90)(91)(92). In whole-plasmid transcriptional studies, an additional gene, shiF, was upregulated during growth in human serum and urine and in a neonatal rat sepsis model (93,94).…”

mentioning

confidence: 99%

Plasmid-Encoded Iron Uptake Systems

Lorenzo

Stork

2014

Microbiol Spectr

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Plasmids confer genetic information that benefits the bacterial cells containing them. In pathogenic bacteria, plasmids often harbor virulence determinants that enhance the pathogenicity of the bacterium. The ability to acquire iron in environments where it is limited, for instance the eukaryotic host, is a critical factor for bacterial growth. To acquire iron, bacteria have evolved specific iron uptake mechanisms. These systems are often chromosomally encoded, while those that are plasmid-encoded are rare. Two main plasmid types, ColV and pJM1, have been shown to harbor determinants that increase virulence by providing the cell with essential iron for growth. It is clear that these two plasmid groups evolved independently from each other since they do not share similarities either in the plasmid backbones or in the iron uptake systems they harbor. The siderophores aerobactin and salmochelin that are found on ColV plasmids fall in the hydroxamate and catechol group, respectively, whereas both functional groups are present in the anguibactin siderophore, the only iron uptake system found on pJM1-type plasmids. Besides siderophore-mediated iron uptake, ColV plasmids carry additional genes involved in iron metabolism. These systems include ABC transporters, hemolysins, and a hemoglobin protease. ColV-and pJM1-like plasmids have been shown to confer virulence to their bacterial host, and this trait can be completely ascribed to their encoded iron uptake systems.

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Gut microbiota enhance energy accumulation of black-necked crane to cope with impending migration

Zhao

Tang

et al. 2023

Appl Microbiol Biotechnol

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Aerobactin Synthesis Genes iucA and iucC Contribute to the Pathogenicity of Avian Pathogenic Escherichia coli O2 Strain E058

Cited by 46 publications

References 43 publications

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Studies on avian pathogenic Escherichia coli in commercial broiler chicken in South East Queensland

Plasmid-Encoded Iron Uptake Systems

Gut microbiota enhance energy accumulation of black-necked crane to cope with impending migration

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