A total of 80 Escherichia coli strains were examined for expression of P‐fimbriae, mannose‐sensitive haemagglutination (MSHA) and mannose‐resistant haemagglutination (MRHA) of human group A erythrocytes and guinea pig erthrocytes, cell surface hydrophobicity and resistance to serum bactericidal activity. Isolates were obtained from urine of children and adults, either with acute pyelonephritis (n = 15 and n = 12) or lower urinary tract infection (UTI) (n = 30 and n = 23, respectively). Results obtained showed that, in E. coli strains isolated both from children and adults with lower UTI, significant differences were not found concerning the incidence of P‐fimbriae, cell surface hydrophobicity and serum resistance. In pyelonephritogenic E. coli isolated from children and adults, the incidence of P‐fimbriae and cell surface hydrophobicity was associated more frequently with the former (87% vs. 42% and 100% vs. 67%, P < 0.05), while serum resistance was associated with the latter (47% vs. 67%, P < 0.05).