Aeroallergens: a comparative study of two monitoring methods

Plaza, Maria Pilar; Alcázar, Pilar; Velasco-Jiménez, M. J.; Galán, Carmen

doi:10.1007/s10453-017-9475-5

Cited by 10 publications

(6 citation statements)

References 41 publications

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“…Comparable results have been observed when comparing different samplers in the same place and years, i.e. Cyclone collector and Chemvol® high-volume cascade impactor in Córdoba (Plaza et al 2017).…”

Section: Introductionmentioning

confidence: 69%

“…In the last two decades, the Aerobiology focus the research on both, airborne pollen behaviour and aeroallergens (i.e. Moreno-Grau et al, 2006;De Linares et al, 2010;Jato et al, 2010;Galán et al, 2013;Buters et al 2012Buters et al , 2015Alcázar et al 2015;Plaza et al 2016aPlaza et al , 2017. Knowledge on the dynamic of these particles is contributing to major information on airborne biological pollution.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Parietaria major allergens vs pollen in the air we breathe

Linares

Alcázar

Valle

et al. 2019

Environmental Research

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Section: Introductionmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Parietaria major allergens vs pollen in the air we breathe

Linares

Alcázar

Valle

et al. 2019

Environmental Research

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“…In the last decade, there has been a growing interest in the measurement of airborne pollen allergen concentrations, to compare it to the atmospheric levels of their respective pollen grains. These studies found out that the concentration of pollen allergens transported by smaller carriers is independent from the airborne pollen concentration, and that there is often a mismatch between a pollen type's peak and its allergens' peaks [9,[28][29][30][31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

“…To accurately sample and analyse airborne allergens, it is necessary to employ volumetric air samplers with a lower diameter cut-off than those employed for pollen sampling, using different sampling substrates and with higher air volume intakes [8,32]. In fact, while pollen grains belong to the aerosol fraction with a diameter greater than 10 µm (PM 10 ), the other pollen allergens carriers have smaller, more variable sizes, and they can even be found in the finest particles fraction (PM 2.5 and PM 1 ).…”

Section: Introductionmentioning

confidence: 99%

Airborne Pollen, Allergens, and Proteins: A Comparative Study of Three Sampling Methods

et al. 2022

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Nowadays, there is a wide range of different methods available for the monitoring of pollen and allergens, but their relative efficiency is sometimes unclear, as conventional pollen monitoring does not thoroughly describe pollen allergenicity. This study aims to evaluate airborne pollen, allergen, and protein levels, associating them with meteorological and chemical parameters. The sampling was performed in Bologna (Italy) during the grass flowering period, with three different devices: a Cyclone sampler (CS), a Dicothomous sampler (DS), and a Berner impactor (BI). Total proteins were extracted from aerosol samples, and grass allergens Phl p 1 and Phl p 5 were quantified by ELISA. Airborne Poaceae pollen concentrations were also evaluated, using a Hirst-type trap. Proteins and allergens collected by CS resulted about ten times higher than those collected by the other two instruments, possibly due to their different cut-offs, while DS and BI results appeared consistent only for the total proteins collected in the fine fraction (1.3 vs. 1.6 μg/m3). Airborne proteins correlated neither with Poaceae pollen nor with its aeroallergens, while aeroallergens correlated with pollen only in the coarse particulate, indicating the presence of pollen-independent aeroallergens in the fine particulate, promoted by high wind speed.

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“…For these reasons, the dissection of the presence of the diverse grass pollen taxa in the atmosphere of a certain area would be crucial in modern and personalized medical approaches. Airborne allergen monitoring would also be an option (Chapman, ), but it requires additional and specific equipment (Plaza, Alcázar, Velasco‐Jiménez, & Galán, ).…”

Section: Introductionmentioning

confidence: 99%

New biomolecular tools for aerobiological monitoring: Identification of major allergenic Poaceae species through fast real‐time PCR

Ghitarrini

Pierboni

Rondini

et al. 2018

Ecology and Evolution

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Grasses (Poaceae) are very common plants, which are widespread in all environments and urban areas. Despite their economical importance, they can represent a problem to humans due to their abundant production of allergenic pollen. Detailed information about the pollen season for these species is needed in order to plan adequate therapies and to warn allergic people about the risks they take in certain areas at certain moments. Moreover, precise identification of the causative species and their allergens is necessary when the patient is treated with allergen‐specific immunotherapy. The intrafamily morphological similarity of grass pollen grains makes it impossible to distinguish which particular species is present in the atmosphere at a given moment. This study aimed at developing new biomolecular tools to analyze aerobiological samples and identifying major allergenic Poaceae taxa at subfamily or species level, exploiting fast real‐time PCR. Protocols were tested for DNA extraction from pollen sampled with volumetric and gravimetric methods. A fragment of the matK plastidial gene was amplified and sequenced in Poaceae species known to have high allergological impact. Species‐ and subfamily‐specific primer–probe systems were designed and tested in fast real‐time PCRs to evaluate the presence of these taxa in aerobiological pollen samples. Species‐specific systems were obtained for four of five studied species. A primer–probe set was also proposed for the detection of Pooideae (a grass subfamily that includes also major cereal grains) in aerobiological samples, as this subfamily includes species carrying both grass allergens from groups 1 and 5. These, among the 11 groups in which grass pollen allergens are classified, are considered responsible for the most frequent and severe symptoms.

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Aeroallergens: a comparative study of two monitoring methods

Cited by 10 publications

References 41 publications

Parietaria major allergens vs pollen in the air we breathe

Parietaria major allergens vs pollen in the air we breathe

Airborne Pollen, Allergens, and Proteins: A Comparative Study of Three Sampling Methods

New biomolecular tools for aerobiological monitoring: Identification of major allergenic Poaceae species through fast real‐time PCR

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