Aerial flow of barley yellow dwarf viruses and of their vectors in western France

Quillec, F. Leclercq‐Le; Tanguy, Sylvie; Dedryver, C. A.

doi:10.1111/j.1744-7348.1995.tb05004.x

Cited by 26 publications

(12 citation statements)

References 42 publications

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“…Detection of virus antigen in leaves of test plants was done by TAS-ELISA as described for BYDV (Torrance et al 1986;Leclercq-Le Quillec et al 1995). Anti PAV-like purified IgG was produced by INRA, (former Laboratoire de Virologie, Versailles, France) and anti-PAV monoclonal antibody Mac91 was purchased from Neogen Europe ® Ltd (Ayr, Scotland, UK).…”

Section: Virus Detectionmentioning

confidence: 99%

Reduced BYDV–PAV transmission by the grain aphid in a Triticum monococcum line

Tanguy

Dedryver

2008

Eur J Plant Pathol

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The luteovirus Barley yellow dwarf virus-PAV (BYDV-PAV) and its vector, the aphid Sitobion avenae are two major sources of yield losses in cereal crops. We report in this paper the effects of a Triticum monococcum line (TM44), resistant by antibiosis to S. avenae, on the different steps of transmission of one BYDV-PAV isolate by the aphids. First, it was shown that TM44 is strongly resistant to BYDV-PAV transmission, but exclusively when S. avenae is the vector. Second, that TM44 is resistant (1) to BYDV-PAV acquisition by S. avenae and (2) to its inoculation, whatever the respective duration of these two periods. Third, that both resistances have partially additive effects. In the discussion, several lines of evidence are given to support the hypothesis that resistance of TM44 to PAV transmission is due to the same disturbances to S. avenae feeding behaviour that are involved in its antibiosis against this aphid species. Reasons for caution in releasing resistant material in the field are presented.

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Section: Virus Detectionmentioning

confidence: 99%

Reduced BYDV–PAV transmission by the grain aphid in a Triticum monococcum line

Tanguy

Dedryver

2008

Eur J Plant Pathol

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“…The English green aphid, Sitobion avenae (Fabricius), considered being a one of the most important pests of wheat in Asia and Europe [30][31][32]. It could cause heavy economic losses to the wheat, both as a phloem feeder and serving as a vector of plant viruses [33][34][35]. It was reported that the wheat (Triticum aestivum, AABBDD) genes Tafps1 and Tafps2 played important roles in induced responses to aphid infestation and in sesquiterpene synthesis [26].…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: De novo assembly and comparative transcriptome analysis revealed the genes that were potentially involved in defensive terpenoids emission in the wheat against the wheat aphid Sitobion avenae (Fabricius)

Zhao

Liu

Zhan

et al. 2019

Preprint

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Background Terpenoid volatiles play an important role directly or indirectly in the plant defense mechanisms against herbivores, including the gramineous crops such as transgenic rice and corn. The conventional varieties of wheat are important gramineous cereal crops that lack aphid-resistant genes. Therefore, it is necessary to seek the aphid-resistant genes by screening for potential terpenoid synthase genes in the wheat germplasm resources. Results The result showed that aphid-damaged Octoploid Tirtitrigia emitted a higher amount of S-linalool, ent-kaurene, (+)-delta-cadinene, (3S,6E)-nerolidol as compared to the intact plant. In addition, (E)-β-caryophyllene, β‐Myrcene, (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) were new volatile terpenoids emitted by the damaged plant. Further olfactory responses tests showed that S-linalool significantly repelled Sitobion avenae (Fabricius). After de novo assembly and quantitative assessment, a total of 182348 (74.8%) unigenes were annotated by alignment with the public protein databases. Of these unigenes, 2389 differentially expressed genes were identified between the intact and damaged ears of Octoploid Trititrigia. The expression profile of 10 randomly selected TPSs was confirmed with RT-qPCR. Candidate genes involved in terpenes biosynthesis were identified by showing the significant transcript changes between the intact and damaged plant ears of Octoploid Trititrigia. The transcript abundances of terpenes biosynthetic pathway-related genes were also positively correlated with the production of volatile terpenoids in the ears. The unigenes of S-linalool synthase gene was mapped to the cloned cDNA WT008_M07 (AK333728) and WT013_P07 (AK335856) of the Chinese spring wheat cultivar. The predicted protein complete ORF sequence (TaLIS1/2) when compared with the S-linalool synthase gene of other species, contained an aspartate-rich region DDxxD motif. Its function was characterized as coordinating the divalent metal ions involved in substrate binding. Furthermore, the phylogenetic tree results indicated that the TaLIS1 and TuNES1 are highly homologous. Conclusions This assembled transcriptome of S. avenae-damaged Octoploid Trititrigia and the intact ears could provide more molecular resources for the future functional characterization analysis of genomics in volatile terpenoids involved in direct or indirect defenses. Our study describes the metabolic regulation mechanism of the volatile terpenoids in the gramineous crops, which provides support for both breeding and genetic modification of the wheat varieties resistant to wheat aphid.

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“…The English green aphid, Sitobion avenae (Fabricius), is considered one of the most important pests of wheat in Asia and Europe [30][31][32]. It can cause heavy economic damage to wheat, both as a phloem feeder and as a vector of plant viruses [33][34][35]. It was reported that the wheat (T. aestivum, AABBDD) gene Tafps1 and Tafps2 played important roles in induced responses to aphid infestation and in sesquiterpene synthesis [26].…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: De novo assembly and comparative transcriptome analysis reveal genes potentially involved in changes of terpenoids in wheat germplasm resource, that defend against the wheat aphid Sitobion avenae (Fabricius)

Zhao¹,

Liu²,

Zhan³

et al. 2019

Preprint

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Background Terpenoid volatiles play an important role in direct and indirect plant defense responses against herbivores, including in gramineous crops such as transgenic rice and corn. The conventional varieties of wheat, an important gramineous cereal crop, lack aphid-resistant genes. It is therefore, necessary to seek aphid-resistant genes by screening for potential terpenoid synthase genes in wheat germplasm resources. Results The result showed that aphid-damaged Octoploid Tirtitrigia emitted a higher amount of S-linalool, ent-kaurene, (+)-delta-cadinene, (3S,6E)- nerolidol compared to intact plant. In addition, (E)-β-caryophyllene, β‐Myrcene, (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) were new volatile terpenoids emitted by the damaged plant. Further olfactory responses tests showed that S-linalool significantly repelled Sitobion avenae (Fabricius). Using the Illumina sequencing platform, approximately 203.09 million high quality paired-end reads were obtained. After de novo assembly and quantitative assessment, a total of 182,348(74.8%) unigenes were annotated by alignment with public protein databases. Of these unigenes, 2,389 differentially expressed genes were identified between intact and damaged ears of Octoploid Trititrigia. The expression profile of 10 randomly selected TPSs was confirmed with RT-qPCR. Candidate genes involved in terpenes biosynthesis were identified showing significant transcript changes between intact and damaged plant ears of Octoploid Trititrigia. Also transcript abundances of terpenes biosynthetic pathway-related genes were positively correlated with the production of volatile terpenoids in ears. The unigenes of S-linalool synthase gene was mapped to the cloned cDNA WT008_M07 (AK333728) and WT013_P07 (AK335856) of the Chinese spring wheat cultivar. The predicted protein complete ORF sequence (TaLIS1/2) when compared with the S-linalool synthase gene of other species, contained an aspartate-rich region DDxxD motif. Its function was characterized as coordinating the divalent metal ions involved in substrate binding. Furthermore, phylogenetic tree results showed that the TaLIS1 and TuNES1 are highly homologous. Conclusions This assembled transcriptome of S. avenae-damaged Octoploid Trititrigia and intact ears can provide more molecular resources for future functional characterization analysis of genomics in volatile terpenoids involved in direct or indirect defenses. Our study describes the metabolic regulation mechanism of volatile terpenoids in gramineous crops, which provides support for both breeding and genetic modification of wheat varieties resistant to wheat aphids.

show abstract

Aerial flow of barley yellow dwarf viruses and of their vectors in western France

Cited by 26 publications

References 42 publications

Reduced BYDV–PAV transmission by the grain aphid in a Triticum monococcum line

Reduced BYDV–PAV transmission by the grain aphid in a Triticum monococcum line

WITHDRAWN: De novo assembly and comparative transcriptome analysis revealed the genes that were potentially involved in defensive terpenoids emission in the wheat against the wheat aphid Sitobion avenae (Fabricius)

WITHDRAWN: De novo assembly and comparative transcriptome analysis reveal genes potentially involved in changes of terpenoids in wheat germplasm resource, that defend against the wheat aphid Sitobion avenae (Fabricius)

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