“…The genome of A. niger contains two aegerolysin and two MACPF-like genes Proteins encoded by genes derived from the two genomes of the fungus A. niger (CBS 513.88 [Pel et al, 2007] and ATTC 1015 [Andersen et al, 2011]) were searched with BLASTP algorithm using amino acid sequences of three fungal aegerolysins, OlyA from P. ostreatus, AspHS and AspHS-like from A. fumigatus, and of two fungal proteins with MACPF domain, PlyB from P. ostreatus and SpoC1-C1C of A. nidulans. Ostreolysin A, besides its highly homologous variant pleurotolysin A, till now remains the best functionally characterised protein from aegerolysin family (Sepčić et al, 2004, Tomita et al, 2004, Ota et al, 2013, Bhat et al, 2015, Lukoyanova et al, 2015, Butala et al, 2017, Panevska et al, 2019. AspHS and AspHS-like proteins from A. fumigatus, belonging to Aspergillus genus, were chosen as other well-studied aegerolysins (Sakaguchi et al, 1975;Ebina et al, 1982;Wartenberg et al, 2011;Rementeria et al, 2005).…”