AEG-1 is associated with tumor progression in nonmuscle-invasive bladder cancer

Yang, Guoliang; Zhang, Lianhua; Sun, Lin; Li, Linfeng; Liu, Mengyao; Chen, Haige; Cao, Ming; Liu, Dongming; Huang, Yiran; Jiang, Bo

doi:10.1007/s12032-014-0986-4

Cited by 17 publications

(17 citation statements)

References 23 publications

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“…MTDH, also known as astrocyte elevated gene-1, is first discovered in human foetal astrocytes in 2002 (41). MTDH is a multifunctional oncogene and overexpressed in multiple types of human cancer, including glioma (42), breast cancer (43), gastric cancer (44), colorectal cancer (45), cervical cancer (46) and bladder cancer (47). MTDH is correlated with tumorigenesis and tumour development by regulating cell groWTh, cell cycle, apoptosis, metastasis, epithelial-to-mesenchymal transition, chemoresistance and angiogenesis (48)(49)(50)(51).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-584 inhibits cell proliferation and invasion in non-small cell lung cancer by directly targeting MTDH

Zhang

Wang

2017

Exp Ther Med

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Abstract. Lung cancer is the third most frequent human malignant tumour and the leading cause of cancer-associated mortality worldwide. Emerging lines of evidence have demonstrated that microRNAs (miRNAs) are upregulated or downregulated in non-small cell lung cancer (NSCLC), and this phenomenon is involved in the regulation of various processes during tumorigenesis and progression, including tumour groWTh, apoptosis, cell invasion, and tumour metastasis. Therefore, understanding the molecular mechanism that associates abnormally expressed miRNAs with NSCLC formation and development may lead to the identification of novel diagnostic, and therapeutic targets for patients with NSCLC. miRNA-584 (miR-584) functions as a tumour suppressor in several types of cancer. However, the expression pattern, detailed biological function and underlying molecular mechanism of miR-584 in NSCLC remain unclear. Therefore, the present study detected the expression of miR-584 in NSCLC, investigated its role in NSCLC cells and determined its underlying molecular mechanism. In the current study, it was demonstrated that miR-584 was downregulated in NSCLC tissues and cell lines. Low miR-584 expression was correlated with tumour size, tumour node metastasis stage and distant metastasis. Overexpression of miR-584 inhibited cell proliferation and invasion in NSCLC. Additionally, metadherin was identified as a direct target gene of miR-584 in NSCLC as confirmed by a series of experiments. Moreover, upregulation of miR-584 was involved in the regulation of the phosphatase and tensin homolog/Akt serine/threonine kinase signalling pathway in NSCLC. Thus, miR-584 may serve as a tumor-suppressor, and the results of the present study provide a reference for future research into the potential mechanisms underlying NSCLC progression.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-584 inhibits cell proliferation and invasion in non-small cell lung cancer by directly targeting MTDH

Zhang

Wang

2017

Exp Ther Med

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“…5a and Supplementary Tables S4 and S5). Interestingly, Metadherin (MTDH) (also referred to as AEG-1 and “Protein LYRIC”), which has previously been shown to be up-regulated in BC and associated with BC progression 43, 44 , was significantly associated with LINC00958. Using Western blotting, we confirmed the physical association between MTDH and LINC00958 in the pull-down samples.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies have identified the MTDH protein in the nucleus as well as the cytoplasm depending on the examined cell types 54 . However, in BC the up-regulated MTDH protein is primarily found in the cytoplasm 44 . Nevertheless further analyses are required to elucidate the mechanisms of action and functional role of the MTDH-LINC00958 association (as well as the other associations identified in the RIP analysis).…”

Section: Discussionmentioning

confidence: 98%

Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer

Seitz

Christensen

et al. 2017

Sci Rep

107

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Aberrant expression of long non-coding RNAs (lncRNAs) has been regarded as a critical component in bladder cancer (BC) and lncRNAs have been associated with BC development and progression although their overall expression and functional significance is still unclear. The aim of our study was to identify novel lncRNAs with a functional role in BC carcinogenesis. RNA-sequencing was used to identify aberrantly expressed lncRNAs in 8 normal and 72 BC samples. We identified 89 lncRNAs that were significantly dys-regulated in BC. Five lncRNAs; LINC00958, LINC01296, LINC00355, LNC-CMC1-1 and LNC-ALX1-2 were selected for further analyses. Silencing of LINC00958 or LINC01296 in vitro reduced both cell viability and migration. Knock-down of LINC00958 also affected invasion and resistance to anoikis. These cellular effects could be linked to direct/indirect regulation of protein coding mRNAs involved in cell death/survival, proliferation and cellular movement. Finally, we showed that LINC00958 binds proteins involved in regulation and initiation of translation and in post-transcriptional modification of RNA, including Metadherin, which has previously been associated with BC. Our analyses identified novel lncRNAs in BC that likely act as oncogenic drivers contributing to an aggressive cancerous phenotype likely through interaction with proteins involved in initiation of translation and/or post-transcriptional modification of RNA.

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“…10 Moreover, the expression level of AEG-1 has been found to correlate with the clinical stage, differentiation degree, tumour grade, tumour metastasis, and survival time of patients. 3,[5][6][7][11][12][13][14][15] As a multifunctional gene, AEG-1 plays important roles in tumour growth, metastasis, angiogenesis, and drug resistance, thus contributing to tumorigenesis. [16][17][18][19][20] The expression level of AEG-1 is aberrantly high in melanoma.…”

mentioning

confidence: 99%

Silencing of astrocyte elevated gene‐1 inhibits proliferation and migration of melanoma cells and induces apoptosis

Zhang

Peng

Wang

et al. 2017

Clin Exp Pharma Physio

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Melanoma is an aggressive skin malignancy with a high mortality. Astrocyte elevated gene-1 (AEG-1), a downstream target of Ras and c-Myc, has been implicated in the development of multiple tumours, but its role in melanoma remains unclear. In the present study, the role of AEG-1 in melanoma was explored through AEG-1 silencing. Our results showed that silencing AEG-1 inhibited the proliferation of melanoma cells, induced cell cycle arrest, and reduced levels of cyclin A, cyclin B, cyclin D1, cyclin E, and cyclin-dependent kinase 2. AEG-1silencing also induced apoptosis in melanoma cells and altered the levels of cleaved caspase-3, B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein. Moreover, silencing AEG-1 suppressed the migration and invasion of melanoma cells, reduced the expressions and activities of matrix metallopeptidase (MMP)-2 and MMP-9, and inhibited the activation of the Wnt/β-catenin signalling pathway in melanoma cells. Furthermore, in vivo experiments revealed that AEG-1 silencing inhibited the growth of melanoma xenografts in nude mice. In summary, our study demonstrates an oncogenic role of AEG-1 in melanoma and suggests that AEG-1 may serve as a potential therapeutic target in the treatment of melanoma.

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AEG-1 is associated with tumor progression in nonmuscle-invasive bladder cancer

Cited by 17 publications

References 23 publications

MicroRNA-584 inhibits cell proliferation and invasion in non-small cell lung cancer by directly targeting MTDH

MicroRNA-584 inhibits cell proliferation and invasion in non-small cell lung cancer by directly targeting MTDH

Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer

Silencing of astrocyte elevated gene‐1 inhibits proliferation and migration of melanoma cells and induces apoptosis

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